Candidatus Desulfovibrio trichonymphae
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1079 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1J1DT82|A0A1J1DT82_9DELT Adenylylsulfate reductase subunit A OS=Candidatus Desulfovibrio trichonymphae OX=1725232 GN=aprA PE=4 SV=1
MM1 pKa = 7.53 GYY3 pKa = 10.17 SVTVDD8 pKa = 2.82 TDD10 pKa = 3.32 KK11 pKa = 11.47 CVGCGEE17 pKa = 4.55 CVDD20 pKa = 3.86 VCPVAVYY27 pKa = 9.36 EE28 pKa = 4.32 IKK30 pKa = 10.58 DD31 pKa = 3.91 GKK33 pKa = 10.44 SEE35 pKa = 3.88 PANSEE40 pKa = 3.77 EE41 pKa = 4.35 CLGCEE46 pKa = 4.14 SCIEE50 pKa = 4.07 VCEE53 pKa = 4.04 ASAIVVEE60 pKa = 4.75 EE61 pKa = 4.02 NN62 pKa = 3.1
Molecular weight: 6.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.932
IPC2_protein 3.846
IPC_protein 3.681
Toseland 3.528
ProMoST 3.834
Dawson 3.643
Bjellqvist 3.821
Wikipedia 3.554
Rodwell 3.528
Grimsley 3.452
Solomon 3.617
Lehninger 3.567
Nozaki 3.795
DTASelect 3.859
Thurlkill 3.579
EMBOSS 3.567
Sillero 3.795
Patrickios 0.006
IPC_peptide 3.617
IPC2_peptide 3.77
IPC2.peptide.svr19 3.758
Protein with the highest isoelectric point:
>tr|A0A1J1E1I5|A0A1J1E1I5_9DELT L-aspartate oxidase OS=Candidatus Desulfovibrio trichonymphae OX=1725232 GN=nadB PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.07 QPSKK9 pKa = 9.07 IRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.96 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MASPGGRR28 pKa = 11.84 AVLCRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.0 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 10.891
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.954
Rodwell 12.135
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.871
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.101
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1079
0
1079
366380
37
1404
339.6
37.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.089 ± 0.098
1.809 ± 0.038
5.177 ± 0.054
5.74 ± 0.07
3.931 ± 0.051
7.898 ± 0.075
2.262 ± 0.029
4.932 ± 0.061
4.119 ± 0.062
10.989 ± 0.081
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.703 ± 0.032
3.168 ± 0.041
4.886 ± 0.047
3.183 ± 0.04
6.907 ± 0.064
5.296 ± 0.041
5.003 ± 0.037
7.308 ± 0.066
1.149 ± 0.032
2.45 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here