Mycobacterium virus Wonder

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 33 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F1BCJ7|F1BCJ7_9CAUD Gp24 OS=Mycobacterium virus Wonder OX=927988 GN=MphageW_gp24 PE=4 SV=1
MM1 pKa = 7.77ALNDD5 pKa = 4.19DD6 pKa = 4.47AVLTAAVGYY15 pKa = 10.54VYY17 pKa = 8.9TAPVGTAAPTPAQLKK32 pKa = 8.04TLNLTDD38 pKa = 3.5TGLWTPTGWDD48 pKa = 3.87SIGHH52 pKa = 5.38TSRR55 pKa = 11.84GDD57 pKa = 3.33MPEE60 pKa = 3.91FGFDD64 pKa = 3.44GGDD67 pKa = 3.49TEE69 pKa = 6.37VRR71 pKa = 11.84GSWQKK76 pKa = 10.88KK77 pKa = 6.91KK78 pKa = 10.53LRR80 pKa = 11.84EE81 pKa = 4.11VTTEE85 pKa = 4.06DD86 pKa = 3.42PVDD89 pKa = 3.66YY90 pKa = 9.4LTLFLHH96 pKa = 6.35QFDD99 pKa = 4.04EE100 pKa = 4.7QAFEE104 pKa = 4.52LYY106 pKa = 10.53YY107 pKa = 10.76GANASTTPGVFGVSAASGDD126 pKa = 3.81PTEE129 pKa = 4.24KK130 pKa = 10.74AFLVVIVDD138 pKa = 3.19GDD140 pKa = 3.73EE141 pKa = 3.86RR142 pKa = 11.84VGFHH146 pKa = 5.6AHH148 pKa = 6.41KK149 pKa = 10.65ASVRR153 pKa = 11.84RR154 pKa = 11.84DD155 pKa = 3.46DD156 pKa = 5.93AIQLPTDD163 pKa = 4.21DD164 pKa = 5.05FAALPVRR171 pKa = 11.84ATFLQHH177 pKa = 6.55NNEE180 pKa = 4.98LLFSWINEE188 pKa = 3.79DD189 pKa = 3.5LFNVEE194 pKa = 4.52EE195 pKa = 4.25EE196 pKa = 4.47EE197 pKa = 4.32EE198 pKa = 4.15

Molecular weight: 21.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F1BCL4|F1BCL4_9CAUD Gp62 OS=Mycobacterium virus Wonder OX=927988 GN=MphageW_gp62 PE=4 SV=1
MM1 pKa = 7.21STTKK5 pKa = 10.41RR6 pKa = 11.84KK7 pKa = 9.32PSHH10 pKa = 6.64RR11 pKa = 11.84NQDD14 pKa = 3.2RR15 pKa = 11.84AHH17 pKa = 6.33KK18 pKa = 10.15RR19 pKa = 11.84KK20 pKa = 9.45PCVDD24 pKa = 3.95CVAEE28 pKa = 4.98GITTKK33 pKa = 10.52RR34 pKa = 11.84KK35 pKa = 9.77APHH38 pKa = 6.61PGPRR42 pKa = 11.84CATHH46 pKa = 6.41HH47 pKa = 5.7RR48 pKa = 11.84AKK50 pKa = 10.4RR51 pKa = 11.84AQRR54 pKa = 11.84RR55 pKa = 11.84DD56 pKa = 3.66TSWEE60 pKa = 3.62TRR62 pKa = 11.84IFATYY67 pKa = 10.21GITAEE72 pKa = 4.52EE73 pKa = 4.37YY74 pKa = 7.55WAIYY78 pKa = 8.55EE79 pKa = 4.23FQGGRR84 pKa = 11.84CYY86 pKa = 10.77GWRR89 pKa = 11.84RR90 pKa = 11.84ANGKK94 pKa = 9.51RR95 pKa = 11.84KK96 pKa = 9.61RR97 pKa = 11.84LSVDD101 pKa = 3.0HH102 pKa = 6.96DD103 pKa = 4.0HH104 pKa = 6.55EE105 pKa = 4.65TGIVRR110 pKa = 11.84GLLCTACNRR119 pKa = 11.84NVLGHH124 pKa = 6.67LRR126 pKa = 11.84DD127 pKa = 3.53EE128 pKa = 4.59RR129 pKa = 11.84EE130 pKa = 3.78AFQRR134 pKa = 11.84FIDD137 pKa = 4.12YY138 pKa = 10.52LDD140 pKa = 4.23SPPAVQVLGEE150 pKa = 4.74RR151 pKa = 11.84ITPDD155 pKa = 2.8MAAA158 pKa = 3.87

Molecular weight: 18.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

33

0

33

9994

121

1008

302.8

33.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.806 ± 0.564

0.8 ± 0.158

6.394 ± 0.337

6.554 ± 0.396

3.612 ± 0.261

8.585 ± 0.497

1.971 ± 0.227

4.733 ± 0.193

4.022 ± 0.225

8.345 ± 0.416

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.411 ± 0.272

3.372 ± 0.274

5.593 ± 0.506

3.932 ± 0.389

6.594 ± 0.436

5.123 ± 0.25

5.944 ± 0.337

7.154 ± 0.252

2.041 ± 0.216

3.012 ± 0.255
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski