Bacillus phage CampHawk

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Spounavirinae; Okubovirus; Bacillus virus Camphawk

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 201 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5PWS0|U5PWS0_9CAUD RNA polymerase sigma factor OS=Bacillus phage CampHawk OX=1406783 GN=CampHawk_124 PE=4 SV=1
MM1 pKa = 7.83SDD3 pKa = 3.62NSNKK7 pKa = 10.01TITISVEE14 pKa = 3.94EE15 pKa = 4.26YY16 pKa = 10.62DD17 pKa = 4.67NSIKK21 pKa = 10.19TITIPLEE28 pKa = 4.35EE29 pKa = 4.34YY30 pKa = 9.76QHH32 pKa = 6.85LIDD35 pKa = 4.63RR36 pKa = 11.84VSFLDD41 pKa = 3.62CLEE44 pKa = 4.31SAGVDD49 pKa = 2.85NWGGYY54 pKa = 10.12GEE56 pKa = 4.15AQEE59 pKa = 4.88MYY61 pKa = 10.69NEE63 pKa = 4.43DD64 pKa = 3.73SEE66 pKa = 5.12EE67 pKa = 4.14EE68 pKa = 4.15TGGYY72 pKa = 9.38

Molecular weight: 8.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5PWQ2|U5PWQ2_9CAUD Endolysin OS=Bacillus phage CampHawk OX=1406783 GN=CampHawk_104 PE=4 SV=1
MM1 pKa = 7.74SDD3 pKa = 3.51IIIPFFTSAVTAFIVAYY20 pKa = 10.73LLDD23 pKa = 3.67RR24 pKa = 11.84WSKK27 pKa = 9.97RR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 9.2

Molecular weight: 3.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

201

0

201

39241

18

1129

195.2

22.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.397 ± 0.167

0.981 ± 0.072

6.71 ± 0.134

8.076 ± 0.254

3.514 ± 0.126

6.728 ± 0.195

1.855 ± 0.093

6.371 ± 0.15

8.129 ± 0.29

8.117 ± 0.174

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.722 ± 0.104

4.949 ± 0.167

3.354 ± 0.118

3.318 ± 0.121

4.577 ± 0.118

6.473 ± 0.202

6.001 ± 0.254

7.273 ± 0.174

1.114 ± 0.067

4.342 ± 0.132
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski