Acidovorax sp. MR-S7
Average proteome isoelectric point is 7.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4762 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A081B621|A0A081B621_9BURK Uncharacterized protein OS=Acidovorax sp. MR-S7 OX=1268622 GN=AVS7_00124 PE=4 SV=1
MM1 pKa = 7.26 SAVAEE6 pKa = 4.15 NTPTDD11 pKa = 3.66 MPAPIVFTDD20 pKa = 3.68 SAAAKK25 pKa = 10.02 VADD28 pKa = 5.45 LIAEE32 pKa = 4.31 EE33 pKa = 4.82 GNPDD37 pKa = 2.99 LKK39 pKa = 11.13 LRR41 pKa = 11.84 VFVQGGGCSGFQYY54 pKa = 10.94 GFTFDD59 pKa = 4.81 EE60 pKa = 4.64 ITNEE64 pKa = 4.77 DD65 pKa = 4.2 DD66 pKa = 3.25 TTMTKK71 pKa = 10.66 NGVSLLIDD79 pKa = 3.54 AMSYY83 pKa = 10.32 QYY85 pKa = 11.5 LVGAEE90 pKa = 3.79 IDD92 pKa = 3.91 YY93 pKa = 11.36 KK94 pKa = 11.19 EE95 pKa = 4.67 DD96 pKa = 3.23 LQGAQFVIKK105 pKa = 10.44 NPNAQTTCGCGSSFSAA121 pKa = 4.79
Molecular weight: 12.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.872
IPC_protein 3.821
Toseland 3.605
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.643
Grimsley 3.528
Solomon 3.795
Lehninger 3.745
Nozaki 3.935
DTASelect 4.151
Thurlkill 3.681
EMBOSS 3.757
Sillero 3.935
Patrickios 1.888
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.844
Protein with the highest isoelectric point:
>tr|A0A0A1VMJ3|A0A0A1VMJ3_9BURK Peptide chain release factor 2 OS=Acidovorax sp. MR-S7 OX=1268622 GN=prfB PE=3 SV=1
MM1 pKa = 6.87 STRR4 pKa = 11.84 RR5 pKa = 11.84 PLVHH9 pKa = 7.15 LLPGLALCGAVTLVSLGLEE28 pKa = 4.08 HH29 pKa = 7.98 VEE31 pKa = 3.95 AALTGRR37 pKa = 11.84 AWLEE41 pKa = 3.68 ALVLSIVIGTLLRR54 pKa = 11.84 TAWKK58 pKa = 9.16 PPASFEE64 pKa = 5.0 PGIQCAAKK72 pKa = 8.99 TLMEE76 pKa = 4.44 AAVMLMGATVSFGAILQAGTPLLAGIVLTVFATIAVSFSLGRR118 pKa = 11.84 FFGLPAKK125 pKa = 9.54 MAALVACGNSICGNSAIAAVAPAIDD150 pKa = 4.08 ADD152 pKa = 4.07 SRR154 pKa = 11.84 DD155 pKa = 3.7 VTTAIAFTAVLGIVVVLLIPVIAHH179 pKa = 5.35 VLHH182 pKa = 6.7 LNAAASGVLAGLTVYY197 pKa = 10.51 AVPQVLAAAAPMGAAAVQVGTLVKK221 pKa = 10.38 LIRR224 pKa = 11.84 VLMLGPVVATLSILYY239 pKa = 9.83 ARR241 pKa = 11.84 SRR243 pKa = 11.84 RR244 pKa = 11.84 SEE246 pKa = 3.86 PRR248 pKa = 11.84 QAAPRR253 pKa = 11.84 RR254 pKa = 11.84 SLTQYY259 pKa = 10.39 LPVFILVFLALALIRR274 pKa = 11.84 SIGLLPQALVAPASSLSGLLTIVAMAGLGLGVDD307 pKa = 4.27 LRR309 pKa = 11.84 SVTAAGPRR317 pKa = 11.84 VSIVVTLSLVVLGGFAMLVLHH338 pKa = 6.72 ALKK341 pKa = 10.58 LAA343 pKa = 4.09
Molecular weight: 34.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.545
IPC_protein 10.379
Toseland 10.555
ProMoST 10.277
Dawson 10.657
Bjellqvist 10.409
Wikipedia 10.877
Rodwell 10.804
Grimsley 10.716
Solomon 10.789
Lehninger 10.745
Nozaki 10.584
DTASelect 10.394
Thurlkill 10.555
EMBOSS 10.95
Sillero 10.599
Patrickios 10.54
IPC_peptide 10.789
IPC2_peptide 9.706
IPC2.peptide.svr19 8.533
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4762
0
4762
1533141
20
1978
322.0
34.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.81 ± 0.057
0.978 ± 0.011
5.027 ± 0.022
5.1 ± 0.034
3.276 ± 0.019
8.656 ± 0.034
2.386 ± 0.023
3.934 ± 0.024
2.878 ± 0.03
10.816 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.434 ± 0.016
2.319 ± 0.024
5.544 ± 0.026
4.326 ± 0.026
7.2 ± 0.041
4.914 ± 0.023
5.073 ± 0.034
7.608 ± 0.034
1.541 ± 0.017
2.18 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here