Cellvibrio sp. PSBB023
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3892 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1U9NDD1|A0A1U9NDD1_9GAMM Uncharacterized protein OS=Cellvibrio sp. PSBB023 OX=1945512 GN=B0D95_16155 PE=4 SV=1
MM1 pKa = 7.95 PSPIFVQITPSEE13 pKa = 4.1 IGSDD17 pKa = 3.82 QILTAQLQVSLQSAAGIVFGLLLNDD42 pKa = 3.36 YY43 pKa = 11.17 DD44 pKa = 5.32 GFPDD48 pKa = 3.93 PNAVNAFPKK57 pKa = 10.17 YY58 pKa = 10.53 NNPTPPEE65 pKa = 4.68 DD66 pKa = 4.64 PDD68 pKa = 3.74 SDD70 pKa = 4.14 PYY72 pKa = 10.68 KK73 pKa = 10.35 CAWDD77 pKa = 3.45 IQSVSNLNTASPLLFQAVYY96 pKa = 9.31 FPQVGEE102 pKa = 4.43 TPDD105 pKa = 3.34 QVKK108 pKa = 8.34 WFLKK112 pKa = 9.92 YY113 pKa = 9.69 SVAGIEE119 pKa = 4.28 HH120 pKa = 7.14 EE121 pKa = 4.3 EE122 pKa = 4.34 SGWAPLGTT130 pKa = 3.89
Molecular weight: 14.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.834
IPC_protein 3.77
Toseland 3.567
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.719
Rodwell 3.605
Grimsley 3.49
Solomon 3.745
Lehninger 3.706
Nozaki 3.897
DTASelect 4.113
Thurlkill 3.643
EMBOSS 3.732
Sillero 3.897
Patrickios 0.769
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.791
Protein with the highest isoelectric point:
>tr|A0A1U9NCR1|A0A1U9NCR1_9GAMM Monovalent cation/H+ antiporter subunit A OS=Cellvibrio sp. PSBB023 OX=1945512 GN=B0D95_15065 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 9.95 RR12 pKa = 11.84 VRR14 pKa = 11.84 NHH16 pKa = 5.46 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.2 SGRR28 pKa = 11.84 AVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.79 GRR39 pKa = 11.84 AEE41 pKa = 4.04 LTRR44 pKa = 11.84 VV45 pKa = 3.16
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.422
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.31
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.047
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.101
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3892
0
3892
1362328
30
3990
350.0
38.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.901 ± 0.048
0.924 ± 0.015
5.567 ± 0.045
5.644 ± 0.041
3.952 ± 0.025
7.048 ± 0.045
2.189 ± 0.022
5.969 ± 0.038
4.46 ± 0.037
10.502 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.275 ± 0.018
4.302 ± 0.039
4.284 ± 0.028
4.547 ± 0.033
5.147 ± 0.037
6.499 ± 0.044
5.559 ± 0.045
6.822 ± 0.038
1.377 ± 0.016
3.029 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here