Cellvibrio sp. PSBB023 
Average proteome isoelectric point is 6.26 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 3892 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A1U9NDD1|A0A1U9NDD1_9GAMM Uncharacterized protein OS=Cellvibrio sp. PSBB023 OX=1945512 GN=B0D95_16155 PE=4 SV=1MM1 pKa = 7.95  PSPIFVQITPSEE13 pKa = 4.1  IGSDD17 pKa = 3.82  QILTAQLQVSLQSAAGIVFGLLLNDD42 pKa = 3.36  YY43 pKa = 11.17  DD44 pKa = 5.32  GFPDD48 pKa = 3.93  PNAVNAFPKK57 pKa = 10.17  YY58 pKa = 10.53  NNPTPPEE65 pKa = 4.68  DD66 pKa = 4.64  PDD68 pKa = 3.74  SDD70 pKa = 4.14  PYY72 pKa = 10.68  KK73 pKa = 10.35  CAWDD77 pKa = 3.45  IQSVSNLNTASPLLFQAVYY96 pKa = 9.31  FPQVGEE102 pKa = 4.43  TPDD105 pKa = 3.34  QVKK108 pKa = 8.34  WFLKK112 pKa = 9.92  YY113 pKa = 9.69  SVAGIEE119 pKa = 4.28  HH120 pKa = 7.14  EE121 pKa = 4.3  EE122 pKa = 4.34  SGWAPLGTT130 pKa = 3.89  
 14.19 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.737 
IPC2_protein 3.834 
IPC_protein 3.77 
Toseland    3.567 
ProMoST     3.948 
Dawson      3.77 
Bjellqvist  3.935 
Wikipedia   3.719 
Rodwell     3.605 
Grimsley    3.49 
Solomon     3.745 
Lehninger   3.706 
Nozaki      3.897 
DTASelect   4.113 
Thurlkill   3.643 
EMBOSS      3.732 
Sillero     3.897 
Patrickios  0.769 
IPC_peptide 3.745 
IPC2_peptide  3.872 
IPC2.peptide.svr19  3.791 
 Protein with the highest isoelectric point: 
>tr|A0A1U9NCR1|A0A1U9NCR1_9GAMM Monovalent cation/H+ antiporter subunit A OS=Cellvibrio sp. PSBB023 OX=1945512 GN=B0D95_15065 PE=4 SV=1MM1 pKa = 7.45  KK2 pKa = 9.61  RR3 pKa = 11.84  TFQPSNLKK11 pKa = 9.95  RR12 pKa = 11.84  VRR14 pKa = 11.84  NHH16 pKa = 5.46  GFRR19 pKa = 11.84  ARR21 pKa = 11.84  MATKK25 pKa = 10.2  SGRR28 pKa = 11.84  AVLARR33 pKa = 11.84  RR34 pKa = 11.84  RR35 pKa = 11.84  SKK37 pKa = 10.79  GRR39 pKa = 11.84  AEE41 pKa = 4.04  LTRR44 pKa = 11.84  VV45 pKa = 3.16  
 5.25 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.422 
IPC2_protein 10.906 
IPC_protein 12.501 
Toseland    12.661 
ProMoST     13.159 
Dawson      12.661 
Bjellqvist  12.661 
Wikipedia   13.13 
Rodwell     12.31 
Grimsley    12.705 
Solomon     13.159 
Lehninger   13.056 
Nozaki      12.661 
DTASelect   12.661 
Thurlkill   12.661 
EMBOSS      13.159 
Sillero     12.661 
Patrickios  12.047 
IPC_peptide 13.159 
IPC2_peptide  12.149 
IPC2.peptide.svr19  9.101 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        3892 
0
3892 
1362328
30
3990
350.0
38.64
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        9.901 ± 0.048
0.924 ± 0.015
5.567 ± 0.045
5.644 ± 0.041
3.952 ± 0.025
7.048 ± 0.045
2.189 ± 0.022
5.969 ± 0.038
4.46 ± 0.037
10.502 ± 0.054
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.275 ± 0.018
4.302 ± 0.039
4.284 ± 0.028
4.547 ± 0.033
5.147 ± 0.037
6.499 ± 0.044
5.559 ± 0.045
6.822 ± 0.038
1.377 ± 0.016
3.029 ± 0.025
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here