Nitrosomonas sp. Nm58

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Nitrosomonadaceae; Nitrosomonas; unclassified Nitrosomonas

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H3Q2D5|A0A1H3Q2D5_9PROT KfrB domain-containing protein OS=Nitrosomonas sp. Nm58 OX=200126 GN=SAMN05421754_105213 PE=4 SV=1
MM1 pKa = 7.65SNLLFNVTGSVDD13 pKa = 4.05DD14 pKa = 4.82DD15 pKa = 4.5LLIGSIGDD23 pKa = 4.71DD24 pKa = 3.06ILTGNLGNDD33 pKa = 3.57TLIGGVGNDD42 pKa = 3.13VAVYY46 pKa = 10.38FDD48 pKa = 4.21DD49 pKa = 3.53QANYY53 pKa = 9.95EE54 pKa = 4.05FSLNFSGQTTVRR66 pKa = 11.84DD67 pKa = 3.57LTTADD72 pKa = 3.63GDD74 pKa = 3.79EE75 pKa = 4.57GTDD78 pKa = 3.29VLLDD82 pKa = 3.37IEE84 pKa = 4.58IVRR87 pKa = 11.84FSDD90 pKa = 3.5GDD92 pKa = 3.73VKK94 pKa = 11.03LSYY97 pKa = 10.47QNSGEE102 pKa = 4.02FHH104 pKa = 6.85INTYY108 pKa = 6.57TTGDD112 pKa = 3.23QFAPEE117 pKa = 3.86ITALTNGGFVVTWTSEE133 pKa = 4.16GQDD136 pKa = 2.75GGAYY140 pKa = 9.79VIYY143 pKa = 9.5GQRR146 pKa = 11.84YY147 pKa = 7.54DD148 pKa = 3.8ANGVAQGNEE157 pKa = 3.94FLVNTTTTSSYY168 pKa = 10.53LPSVTTLTSGGFVVTWSSEE187 pKa = 3.9APGSKK192 pKa = 9.59FHH194 pKa = 7.8IYY196 pKa = 9.38GQRR199 pKa = 11.84FDD201 pKa = 5.15ANGITNGSEE210 pKa = 3.9FLVSTNTTHH219 pKa = 6.03SQSSSSITALTNGGFVVVWISDD241 pKa = 3.61AQDD244 pKa = 3.29GSDD247 pKa = 3.31YY248 pKa = 11.13GIYY251 pKa = 10.08GQRR254 pKa = 11.84FDD256 pKa = 5.34ANGAAQDD263 pKa = 4.12SEE265 pKa = 4.3FHH267 pKa = 6.78INTTTTTTYY276 pKa = 10.32SQSSPLITALTNGGFVVTWISEE298 pKa = 4.25GQDD301 pKa = 3.18SKK303 pKa = 11.66FDD305 pKa = 3.59IYY307 pKa = 10.26GQRR310 pKa = 11.84YY311 pKa = 7.59DD312 pKa = 3.86ANGIAQAGEE321 pKa = 3.88FRR323 pKa = 11.84VNTTTTTTSSLDD335 pKa = 3.42HH336 pKa = 6.92SIVALTNGGFVATWSSGSSSSDD358 pKa = 2.68GFGIYY363 pKa = 8.82GQRR366 pKa = 11.84FDD368 pKa = 4.25VNGVAQGNEE377 pKa = 3.94FLVNTTTTRR386 pKa = 11.84SEE388 pKa = 4.07SSPSITALTNGSFVITWSSVDD409 pKa = 3.36QDD411 pKa = 4.16GKK413 pKa = 10.84NDD415 pKa = 3.08IYY417 pKa = 11.0GQRR420 pKa = 11.84FDD422 pKa = 4.59ANGVAQGSEE431 pKa = 4.27FLVNTYY437 pKa = 8.77TFGNQSTSSTTALTNGGFVVTWVSTDD463 pKa = 3.39QEE465 pKa = 4.49SGDD468 pKa = 3.41VSDD471 pKa = 5.63RR472 pKa = 11.84FFSPGGIYY480 pKa = 9.23GQRR483 pKa = 11.84YY484 pKa = 9.16DD485 pKa = 4.12PEE487 pKa = 4.5GNKK490 pKa = 9.98IGDD493 pKa = 3.62LTLTGDD499 pKa = 3.61ANNNNLHH506 pKa = 5.98VADD509 pKa = 4.06TTIASIRR516 pKa = 11.84LLGMEE521 pKa = 4.71GNDD524 pKa = 3.06RR525 pKa = 11.84LQGSVGNDD533 pKa = 3.15ALDD536 pKa = 3.82GGIGADD542 pKa = 3.65TLIGGLGNDD551 pKa = 3.78TYY553 pKa = 11.31FVDD556 pKa = 3.68NVGDD560 pKa = 3.66IVTEE564 pKa = 4.09SLNAGTDD571 pKa = 3.77TVNSSIDD578 pKa = 3.37YY579 pKa = 7.41TLPTNLEE586 pKa = 4.17NLNMTGTLAINGTGNDD602 pKa = 3.97LDD604 pKa = 4.74NIIIANSADD613 pKa = 3.39NQLNGGAGNDD623 pKa = 3.85TLKK626 pKa = 10.85AGYY629 pKa = 9.92GRR631 pKa = 11.84DD632 pKa = 3.49VLTGGVGDD640 pKa = 5.36DD641 pKa = 3.34IFNFFAVGDD650 pKa = 4.05FVIHH654 pKa = 6.97DD655 pKa = 4.35FVNSSDD661 pKa = 4.18LLVFDD666 pKa = 5.04SKK668 pKa = 11.38ATGLYY673 pKa = 10.24DD674 pKa = 3.54INNLLSVISSIEE686 pKa = 3.96DD687 pKa = 3.26SSEE690 pKa = 3.52GAIVHH695 pKa = 6.84FIDD698 pKa = 4.84NIASITLIGLHH709 pKa = 6.63SDD711 pKa = 3.82DD712 pKa = 5.57LSTSMVGFAA721 pKa = 5.08

Molecular weight: 75.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H3LBM7|A0A1H3LBM7_9PROT Fatty acid desaturase OS=Nitrosomonas sp. Nm58 OX=200126 GN=SAMN05421754_101523 PE=4 SV=1
MM1 pKa = 7.76SADD4 pKa = 4.79QPLAWYY10 pKa = 6.82PVPVTASINIRR21 pKa = 11.84WWLLHH26 pKa = 6.69RR27 pKa = 11.84DD28 pKa = 3.35SLTRR32 pKa = 11.84LIQQRR37 pKa = 11.84CSKK40 pKa = 10.57FSVDD44 pKa = 4.94PLFQSLAPACTDD56 pKa = 3.23EE57 pKa = 4.53LVMMNLRR64 pKa = 11.84RR65 pKa = 11.84GEE67 pKa = 3.92LALVRR72 pKa = 11.84EE73 pKa = 4.58VYY75 pKa = 9.93LYY77 pKa = 11.07CGDD80 pKa = 3.63IPVVFAHH87 pKa = 5.82SVVARR92 pKa = 11.84KK93 pKa = 9.05HH94 pKa = 5.76LQSAWRR100 pKa = 11.84GLSRR104 pKa = 11.84LGNRR108 pKa = 11.84SLGTMLFTNPMIKK121 pKa = 9.75RR122 pKa = 11.84AEE124 pKa = 3.98FGYY127 pKa = 10.15KK128 pKa = 9.15QLNIHH133 pKa = 6.39HH134 pKa = 7.2PLFKK138 pKa = 10.27RR139 pKa = 11.84ACQRR143 pKa = 11.84LQTPPTSLWARR154 pKa = 11.84RR155 pKa = 11.84SLFTLQGQPILVTEE169 pKa = 4.27VFLPAILRR177 pKa = 11.84LKK179 pKa = 10.2II180 pKa = 3.73

Molecular weight: 20.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3100

0

3100

926182

25

3980

298.8

33.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.099 ± 0.048

1.011 ± 0.016

5.135 ± 0.032

6.024 ± 0.042

4.051 ± 0.034

6.956 ± 0.044

2.525 ± 0.022

6.823 ± 0.042

4.699 ± 0.041

10.757 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.484 ± 0.024

3.841 ± 0.03

4.432 ± 0.03

4.222 ± 0.031

5.807 ± 0.037

6.126 ± 0.037

5.258 ± 0.033

6.493 ± 0.033

1.351 ± 0.02

2.905 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski