Achromobacter phage phiAxp-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K2FHU1|A0A0K2FHU1_9CAUD Uncharacterized protein OS=Achromobacter phage phiAxp-2 OX=1664246 GN=ADP64_000045 PE=4 SV=1
MM1 pKa = 7.8TDD3 pKa = 3.09QTNTPNTDD11 pKa = 2.94ANTTIDD17 pKa = 3.44LTAISRR23 pKa = 11.84VIGFLGMFVAHH34 pKa = 6.06VEE36 pKa = 4.34CAVGYY41 pKa = 10.29QYY43 pKa = 11.34GQQRR47 pKa = 11.84PATMEE52 pKa = 4.16GEE54 pKa = 4.32PDD56 pKa = 3.45HH57 pKa = 7.2LDD59 pKa = 3.16QIMADD64 pKa = 3.93KK65 pKa = 10.44EE66 pKa = 4.4PIVAFMGSGASDD78 pKa = 3.56ALFISDD84 pKa = 4.29IEE86 pKa = 4.22AARR89 pKa = 11.84AAYY92 pKa = 8.63MEE94 pKa = 4.14LMKK97 pKa = 10.88VYY99 pKa = 10.35GIEE102 pKa = 4.24DD103 pKa = 3.77AGTANISMQNAPSTDD118 pKa = 3.4TPQQ121 pKa = 4.09

Molecular weight: 12.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K2FI58|A0A0K2FI58_9CAUD Decorator protein D OS=Achromobacter phage phiAxp-2 OX=1664246 GN=ADP64_000016 PE=4 SV=1
MM1 pKa = 7.74ASPYY5 pKa = 10.15LVAVEE10 pKa = 5.39GYY12 pKa = 11.15ADD14 pKa = 4.5ALRR17 pKa = 11.84QIQDD21 pKa = 2.89LDD23 pKa = 3.91GAVSQSARR31 pKa = 11.84QAVNRR36 pKa = 11.84TIDD39 pKa = 3.15WARR42 pKa = 11.84TRR44 pKa = 11.84SAEE47 pKa = 3.97EE48 pKa = 3.58IMRR51 pKa = 11.84QVNFGKK57 pKa = 10.44EE58 pKa = 3.98YY59 pKa = 9.66LTGRR63 pKa = 11.84DD64 pKa = 2.69GRR66 pKa = 11.84LQVRR70 pKa = 11.84KK71 pKa = 9.56KK72 pKa = 10.95ARR74 pKa = 11.84GDD76 pKa = 3.43DD77 pKa = 3.87LEE79 pKa = 5.17AVLVGRR85 pKa = 11.84QRR87 pKa = 11.84ATSLARR93 pKa = 11.84FTDD96 pKa = 3.32TRR98 pKa = 11.84DD99 pKa = 3.26PGVTRR104 pKa = 11.84RR105 pKa = 11.84EE106 pKa = 3.79GGVRR110 pKa = 11.84VEE112 pKa = 4.23VKK114 pKa = 10.29PGSEE118 pKa = 3.64KK119 pKa = 10.19FMKK122 pKa = 10.25RR123 pKa = 11.84AFLMRR128 pKa = 11.84LRR130 pKa = 11.84AGSQLTDD137 pKa = 3.05TKK139 pKa = 11.32YY140 pKa = 11.25NLGLAIRR147 pKa = 11.84LRR149 pKa = 11.84PGEE152 pKa = 3.98KK153 pKa = 9.84LANKK157 pKa = 8.6TRR159 pKa = 11.84IQQINTNLFLLYY171 pKa = 10.29GPSVDD176 pKa = 3.65QVFRR180 pKa = 11.84TVAPEE185 pKa = 3.35IAPRR189 pKa = 11.84VEE191 pKa = 4.8SYY193 pKa = 11.49LEE195 pKa = 4.14TEE197 pKa = 4.62FLRR200 pKa = 11.84LLEE203 pKa = 4.13VQRR206 pKa = 11.84RR207 pKa = 11.84GG208 pKa = 3.26

Molecular weight: 23.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

19247

40

1600

223.8

24.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.958 ± 0.663

0.972 ± 0.135

6.022 ± 0.286

5.84 ± 0.259

3.45 ± 0.201

7.544 ± 0.271

1.595 ± 0.163

4.972 ± 0.145

4.432 ± 0.277

8.256 ± 0.356

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.93 ± 0.155

4.203 ± 0.231

4.941 ± 0.363

4.12 ± 0.253

6.365 ± 0.283

5.315 ± 0.259

6.178 ± 0.293

7.071 ± 0.229

1.756 ± 0.19

3.081 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski