Wuhan insect virus 23

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KLS1|A0A1L3KLS1_9VIRU Uncharacterized protein OS=Wuhan insect virus 23 OX=1923727 PE=4 SV=1
MM1 pKa = 7.34NNLRR5 pKa = 11.84EE6 pKa = 4.64IEE8 pKa = 4.24DD9 pKa = 3.61QLPAQAARR17 pKa = 11.84GPIGAKK23 pKa = 9.15SKK25 pKa = 10.26KK26 pKa = 9.32RR27 pKa = 11.84QGKK30 pKa = 9.27GSSKK34 pKa = 10.12RR35 pKa = 11.84AIEE38 pKa = 4.03RR39 pKa = 11.84FRR41 pKa = 11.84EE42 pKa = 4.19RR43 pKa = 11.84IGTQEE48 pKa = 3.91LDD50 pKa = 3.41EE51 pKa = 4.56FRR53 pKa = 11.84QGILDD58 pKa = 3.43SMMFSRR64 pKa = 11.84DD65 pKa = 3.04EE66 pKa = 4.37GQLSKK71 pKa = 11.05AVNSVQLTQQEE82 pKa = 4.15RR83 pKa = 11.84AIPLTVATRR92 pKa = 11.84GVGFITAIVYY102 pKa = 10.07RR103 pKa = 11.84RR104 pKa = 11.84TCTTWDD110 pKa = 3.25LGAISVIATIYY121 pKa = 9.54QIYY124 pKa = 9.45RR125 pKa = 11.84VHH127 pKa = 6.59LWLVHH132 pKa = 5.69LKK134 pKa = 10.7VYY136 pKa = 10.31LAQQCQVEE144 pKa = 4.61PVTTPGALEE153 pKa = 4.89RR154 pKa = 11.84ITVRR158 pKa = 11.84DD159 pKa = 4.45DD160 pKa = 3.06IRR162 pKa = 11.84EE163 pKa = 3.94LLYY166 pKa = 10.43TVTRR170 pKa = 11.84VPSTLAMILDD180 pKa = 4.53NIGKK184 pKa = 9.56IKK186 pKa = 10.59INDD189 pKa = 4.17RR190 pKa = 11.84IYY192 pKa = 11.23HH193 pKa = 5.55MGYY196 pKa = 10.37AANCPEE202 pKa = 4.18NFASLVITPTTIRR215 pKa = 11.84QILVSLTQEE224 pKa = 3.99ANPDD228 pKa = 2.81IRR230 pKa = 11.84ADD232 pKa = 5.02FIAHH236 pKa = 6.05NSIPGLNVEE245 pKa = 4.97AGFAVNIDD253 pKa = 4.15SVWPADD259 pKa = 4.47AEE261 pKa = 4.13NLLAGDD267 pKa = 3.28VHH269 pKa = 7.87AYY271 pKa = 9.36KK272 pKa = 10.53SWVTRR277 pKa = 11.84VEE279 pKa = 4.01SRR281 pKa = 11.84LPRR284 pKa = 11.84HH285 pKa = 6.08SFSDD289 pKa = 3.74ITWSGQATSGGLWSTEE305 pKa = 3.79RR306 pKa = 11.84LSLCLTSQFTPGRR319 pKa = 11.84ARR321 pKa = 11.84TVRR324 pKa = 11.84RR325 pKa = 11.84RR326 pKa = 11.84DD327 pKa = 3.2AAGEE331 pKa = 3.9LVEE334 pKa = 4.99EE335 pKa = 4.52RR336 pKa = 11.84EE337 pKa = 4.09QRR339 pKa = 11.84EE340 pKa = 3.72AVYY343 pKa = 11.06NGFTLTDD350 pKa = 3.15EE351 pKa = 4.5RR352 pKa = 11.84TTFWSRR358 pKa = 11.84EE359 pKa = 4.1NTPHH363 pKa = 5.33VAIVAGIAGLVGEE376 pKa = 4.69VCHH379 pKa = 7.02LSTRR383 pKa = 11.84HH384 pKa = 6.08EE385 pKa = 3.93IHH387 pKa = 6.28SARR390 pKa = 11.84SVVHH394 pKa = 6.2SPIALMYY401 pKa = 10.69ALTDD405 pKa = 3.55APRR408 pKa = 5.15

Molecular weight: 45.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KLS1|A0A1L3KLS1_9VIRU Uncharacterized protein OS=Wuhan insect virus 23 OX=1923727 PE=4 SV=1
MM1 pKa = 7.83SYY3 pKa = 9.98IGKK6 pKa = 10.17KK7 pKa = 10.17NGFGQRR13 pKa = 11.84FGPARR18 pKa = 11.84PSPYY22 pKa = 10.41LDD24 pKa = 3.76FLAPLAGKK32 pKa = 8.46FEE34 pKa = 4.38RR35 pKa = 11.84SGLSLALVRR44 pKa = 11.84DD45 pKa = 4.64DD46 pKa = 3.77IKK48 pKa = 11.61KK49 pKa = 10.22NMSSKK54 pKa = 10.57SSEE57 pKa = 4.2PTDD60 pKa = 4.61LLYY63 pKa = 10.91QEE65 pKa = 4.71AKK67 pKa = 10.34RR68 pKa = 11.84RR69 pKa = 11.84TLRR72 pKa = 11.84AFMLDD77 pKa = 3.12KK78 pKa = 9.84PVPMIHH84 pKa = 6.69LNDD87 pKa = 3.67VFQEE91 pKa = 4.33DD92 pKa = 4.29LPIWSSSPGLPWMQCGYY109 pKa = 8.54RR110 pKa = 11.84TKK112 pKa = 11.16NDD114 pKa = 2.67IRR116 pKa = 11.84RR117 pKa = 11.84DD118 pKa = 3.13PDD120 pKa = 4.15AINRR124 pKa = 11.84IRR126 pKa = 11.84HH127 pKa = 4.43FWHH130 pKa = 7.13RR131 pKa = 11.84IKK133 pKa = 10.89CFEE136 pKa = 4.15EE137 pKa = 3.93VHH139 pKa = 7.16LPDD142 pKa = 3.6CCAYY146 pKa = 9.87VRR148 pKa = 11.84THH150 pKa = 4.95IVEE153 pKa = 4.2RR154 pKa = 11.84GEE156 pKa = 4.34TKK158 pKa = 10.29VRR160 pKa = 11.84AVWGYY165 pKa = 8.51PATVTFGEE173 pKa = 4.54AVFALPLIQAYY184 pKa = 9.49KK185 pKa = 10.17KK186 pKa = 10.12GGYY189 pKa = 8.54PIAYY193 pKa = 9.66GFEE196 pKa = 4.14TGNAGCKK203 pKa = 10.24KK204 pKa = 8.92MFHH207 pKa = 5.75GMKK210 pKa = 9.85GNHH213 pKa = 5.81YY214 pKa = 10.25LGIDD218 pKa = 3.51FTSFDD223 pKa = 3.48KK224 pKa = 10.83TLPPWLVSTAFDD236 pKa = 3.06ILAYY240 pKa = 10.78NIDD243 pKa = 3.63FLHH246 pKa = 6.25YY247 pKa = 8.82RR248 pKa = 11.84DD249 pKa = 4.15HH250 pKa = 7.25GIARR254 pKa = 11.84VKK256 pKa = 11.2SMVHH260 pKa = 5.29MFNVIKK266 pKa = 10.46DD267 pKa = 3.56YY268 pKa = 11.37AINTKK273 pKa = 9.55IRR275 pKa = 11.84MCNGEE280 pKa = 4.22RR281 pKa = 11.84YY282 pKa = 9.14IKK284 pKa = 10.58KK285 pKa = 10.23SGLASGSYY293 pKa = 7.72FTQLVGSVCNYY304 pKa = 10.88LLLTYY309 pKa = 11.35AMLQLNVEE317 pKa = 4.22INDD320 pKa = 3.43ILVFGDD326 pKa = 4.29DD327 pKa = 4.79SILAVNTAVHH337 pKa = 6.65PDD339 pKa = 3.35EE340 pKa = 4.4VQEE343 pKa = 4.16VLLPLDD349 pKa = 3.13MTVNVKK355 pKa = 10.47KK356 pKa = 10.64SGSSKK361 pKa = 10.61YY362 pKa = 10.26ISNLTFLGYY371 pKa = 10.07KK372 pKa = 9.34ICDD375 pKa = 4.62GIPTRR380 pKa = 11.84DD381 pKa = 3.39RR382 pKa = 11.84EE383 pKa = 4.28KK384 pKa = 10.81QFAAIVWPEE393 pKa = 3.99RR394 pKa = 11.84PDD396 pKa = 3.58KK397 pKa = 11.24CWDD400 pKa = 3.66DD401 pKa = 3.53VASRR405 pKa = 11.84CFGIMYY411 pKa = 10.2ANFGVDD417 pKa = 3.26PVVDD421 pKa = 4.87FWCRR425 pKa = 11.84RR426 pKa = 11.84VVNFRR431 pKa = 11.84PFDD434 pKa = 3.4LHH436 pKa = 5.18MTRR439 pKa = 11.84DD440 pKa = 3.46QQRR443 pKa = 11.84FLDD446 pKa = 4.14LLGLKK451 pKa = 7.84PTTVPTMHH459 pKa = 7.49DD460 pKa = 3.58LSQRR464 pKa = 11.84IGFLAVV470 pKa = 2.94

Molecular weight: 53.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

878

408

470

439.0

49.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.517 ± 1.173

1.822 ± 0.39

5.809 ± 0.912

4.784 ± 1.037

4.442 ± 1.139

6.492 ± 0.238

2.733 ± 0.024

6.72 ± 0.573

4.442 ± 1.299

8.656 ± 0.211

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.392 ± 0.601

3.759 ± 0.214

4.784 ± 0.563

3.417 ± 0.649

7.631 ± 0.938

5.923 ± 0.454

6.264 ± 1.19

7.289 ± 0.201

1.708 ± 0.005

3.417 ± 0.63
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski