Point-Douro narna-like virus
Average proteome isoelectric point is 9.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Z2RSZ2|A0A1Z2RSZ2_9VIRU RdRp OS=Point-Douro narna-like virus OX=2010279 PE=4 SV=1
MM1 pKa = 7.63 PLVARR6 pKa = 11.84 PTSMRR11 pKa = 11.84 VATRR15 pKa = 11.84 SEE17 pKa = 3.73 VSRR20 pKa = 11.84 RR21 pKa = 11.84 VNLAAEE27 pKa = 4.13 TWKK30 pKa = 10.84 AICAAVATRR39 pKa = 11.84 KK40 pKa = 8.85 WPKK43 pKa = 10.22 FNLDD47 pKa = 3.18 FQTDD51 pKa = 3.16 RR52 pKa = 11.84 RR53 pKa = 11.84 RR54 pKa = 11.84 FCTFQALARR63 pKa = 11.84 HH64 pKa = 6.16 LLLSALARR72 pKa = 11.84 GPVEE76 pKa = 3.65 TIKK79 pKa = 10.61 RR80 pKa = 11.84 VKK82 pKa = 10.03 SWTAQIRR89 pKa = 11.84 EE90 pKa = 4.23 DD91 pKa = 3.68 VFLHH95 pKa = 6.17 GRR97 pKa = 11.84 CPPSAFRR104 pKa = 11.84 GLVGALGWTDD114 pKa = 3.78 ANSHH118 pKa = 6.05 LQLSYY123 pKa = 10.46 IGRR126 pKa = 11.84 ALPYY130 pKa = 10.29 GDD132 pKa = 4.04 ATICEE137 pKa = 4.48 HH138 pKa = 6.53 ALKK141 pKa = 10.66 SHH143 pKa = 6.7 ADD145 pKa = 3.47 VLGSLGVTPPSLLGAARR162 pKa = 11.84 GWAKK166 pKa = 10.22 EE167 pKa = 3.61 WAIKK171 pKa = 10.41 NRR173 pKa = 11.84 LSLDD177 pKa = 4.5 LIRR180 pKa = 11.84 EE181 pKa = 4.07 PPIQEE186 pKa = 4.21 SSCLEE191 pKa = 3.64 YY192 pKa = 10.69 SRR194 pKa = 11.84 RR195 pKa = 11.84 EE196 pKa = 3.74 GGYY199 pKa = 9.14 ARR201 pKa = 11.84 AIKK204 pKa = 10.05 EE205 pKa = 3.78 HH206 pKa = 5.53 TARR209 pKa = 11.84 CLTLPPSSVRR219 pKa = 11.84 HH220 pKa = 4.84 SMKK223 pKa = 10.33 EE224 pKa = 3.68 GTRR227 pKa = 11.84 ASNPGRR233 pKa = 11.84 VLKK236 pKa = 10.77 DD237 pKa = 3.05 LAFADD242 pKa = 4.43 LSNTARR248 pKa = 11.84 VVAVPEE254 pKa = 4.4 RR255 pKa = 11.84 GWKK258 pKa = 9.77 ARR260 pKa = 11.84 IVTAHH265 pKa = 6.52 AAARR269 pKa = 11.84 VAFLHH274 pKa = 5.63 SLRR277 pKa = 11.84 HH278 pKa = 5.23 GLFAALRR285 pKa = 11.84 NDD287 pKa = 3.01 RR288 pKa = 11.84 RR289 pKa = 11.84 TKK291 pKa = 10.35 LVAEE295 pKa = 4.53 GLHH298 pKa = 5.97 RR299 pKa = 11.84 EE300 pKa = 4.0 AVEE303 pKa = 3.92 ALFATGVDD311 pKa = 3.79 PRR313 pKa = 11.84 CTIVSADD320 pKa = 3.98 LSAASDD326 pKa = 3.8 TLHH329 pKa = 7.27 GDD331 pKa = 4.04 LLQAIVEE338 pKa = 4.33 GLGDD342 pKa = 4.41 ALGCTSKK349 pKa = 10.96 FMDD352 pKa = 4.24 EE353 pKa = 4.11 FTNAAVGSYY362 pKa = 8.87 TLSYY366 pKa = 10.3 PDD368 pKa = 4.05 GSTVTTQRR376 pKa = 11.84 GALMGLPTTWPLLCLTHH393 pKa = 7.07 LFWCDD398 pKa = 3.08 QSLVQLHH405 pKa = 6.09 GRR407 pKa = 11.84 GGCRR411 pKa = 11.84 EE412 pKa = 3.95 KK413 pKa = 10.13 EE414 pKa = 4.42 TPGSSRR420 pKa = 11.84 EE421 pKa = 4.1 AEE423 pKa = 4.62 VICGDD428 pKa = 4.27 DD429 pKa = 4.46 LAAAWRR435 pKa = 11.84 PDD437 pKa = 2.92 RR438 pKa = 11.84 VAAYY442 pKa = 9.93 EE443 pKa = 4.34 EE444 pKa = 4.33 IAVLCGAVFSPGKK457 pKa = 9.36 HH458 pKa = 5.8 LKK460 pKa = 10.23 SKK462 pKa = 10.57 VYY464 pKa = 10.63 GIFTEE469 pKa = 5.28 DD470 pKa = 3.17 IYY472 pKa = 11.43 SVQVSCVPTRR482 pKa = 11.84 VTRR485 pKa = 11.84 EE486 pKa = 3.69 VSKK489 pKa = 9.14 TWTGPVDD496 pKa = 4.03 KK497 pKa = 11.33 VPMNLVNEE505 pKa = 4.77 LGQLLRR511 pKa = 11.84 WRR513 pKa = 11.84 AQRR516 pKa = 11.84 YY517 pKa = 8.61 KK518 pKa = 10.66 VGKK521 pKa = 8.04 TGAFQFPPRR530 pKa = 11.84 NVTLSYY536 pKa = 10.72 VSKK539 pKa = 9.72 EE540 pKa = 4.18 TGLKK544 pKa = 8.59 MNPVSVLFRR553 pKa = 11.84 EE554 pKa = 4.35 WSTAIPLRR562 pKa = 11.84 WAVRR566 pKa = 11.84 APKK569 pKa = 9.47 HH570 pKa = 5.31 APGMRR575 pKa = 11.84 GDD577 pKa = 4.2 LPPWVTVPLAAHH589 pKa = 6.87 AVASAHH595 pKa = 6.33 PGRR598 pKa = 11.84 WSKK601 pKa = 10.25 VCRR604 pKa = 11.84 VVKK607 pKa = 10.5 LCYY610 pKa = 9.67 PGMAKK615 pKa = 10.21 FFASHH620 pKa = 7.38 GIPPYY625 pKa = 10.31 LPRR628 pKa = 11.84 VLGGGGLPTPLGDD641 pKa = 2.98 RR642 pKa = 11.84 VKK644 pKa = 10.26 IGRR647 pKa = 11.84 VASRR651 pKa = 11.84 RR652 pKa = 11.84 IRR654 pKa = 11.84 KK655 pKa = 8.95 ALGGALYY662 pKa = 10.49 RR663 pKa = 11.84 STDD666 pKa = 3.12 PTTFGSIWTTALSPAYY682 pKa = 10.15 ALALADD688 pKa = 4.35 AQDD691 pKa = 4.5 LDD693 pKa = 4.66 AMHH696 pKa = 7.6 PDD698 pKa = 3.26 AFKK701 pKa = 11.01 VRR703 pKa = 11.84 QTRR706 pKa = 11.84 SLRR709 pKa = 11.84 PPFKK713 pKa = 11.06 DD714 pKa = 2.81 NGNYY718 pKa = 10.35 ADD720 pKa = 4.1 FVEE723 pKa = 4.46 MRR725 pKa = 11.84 AGKK728 pKa = 10.38 GSLWALRR735 pKa = 11.84 QGAQLVGRR743 pKa = 11.84 SFTPSPARR751 pKa = 11.84 VSNALRR757 pKa = 11.84 TKK759 pKa = 9.92 VRR761 pKa = 11.84 KK762 pKa = 9.37 ALAKK766 pKa = 10.0 RR767 pKa = 11.84 GYY769 pKa = 9.25 LRR771 pKa = 11.84 SSAPVSKK778 pKa = 10.46 LIEE781 pKa = 4.25 RR782 pKa = 11.84 ASVKK786 pKa = 10.32 PSDD789 pKa = 3.7 HH790 pKa = 4.59 MWIRR794 pKa = 11.84 EE795 pKa = 3.95 RR796 pKa = 11.84 PVLSFGRR803 pKa = 11.84 FGGRR807 pKa = 11.84 MSGKK811 pKa = 9.82 KK812 pKa = 9.45 EE813 pKa = 4.0 EE814 pKa = 4.28 PVSFQWKK821 pKa = 9.09 KK822 pKa = 10.44 RR823 pKa = 11.84 EE824 pKa = 4.14 SVKK827 pKa = 10.32 TGSS830 pKa = 3.2
Molecular weight: 91.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.289
IPC2_protein 9.414
IPC_protein 9.677
Toseland 10.379
ProMoST 9.984
Dawson 10.496
Bjellqvist 10.175
Wikipedia 10.657
Rodwell 10.833
Grimsley 10.555
Solomon 10.555
Lehninger 10.526
Nozaki 10.394
DTASelect 10.16
Thurlkill 10.379
EMBOSS 10.774
Sillero 10.423
Patrickios 10.496
IPC_peptide 10.555
IPC2_peptide 9.107
IPC2.peptide.svr19 8.475
Protein with the highest isoelectric point:
>tr|A0A1Z2RSZ2|A0A1Z2RSZ2_9VIRU RdRp OS=Point-Douro narna-like virus OX=2010279 PE=4 SV=1
MM1 pKa = 7.63 PLVARR6 pKa = 11.84 PTSMRR11 pKa = 11.84 VATRR15 pKa = 11.84 SEE17 pKa = 3.73 VSRR20 pKa = 11.84 RR21 pKa = 11.84 VNLAAEE27 pKa = 4.13 TWKK30 pKa = 10.84 AICAAVATRR39 pKa = 11.84 KK40 pKa = 8.85 WPKK43 pKa = 10.22 FNLDD47 pKa = 3.18 FQTDD51 pKa = 3.16 RR52 pKa = 11.84 RR53 pKa = 11.84 RR54 pKa = 11.84 FCTFQALARR63 pKa = 11.84 HH64 pKa = 6.16 LLLSALARR72 pKa = 11.84 GPVEE76 pKa = 3.65 TIKK79 pKa = 10.61 RR80 pKa = 11.84 VKK82 pKa = 10.03 SWTAQIRR89 pKa = 11.84 EE90 pKa = 4.23 DD91 pKa = 3.68 VFLHH95 pKa = 6.17 GRR97 pKa = 11.84 CPPSAFRR104 pKa = 11.84 GLVGALGWTDD114 pKa = 3.78 ANSHH118 pKa = 6.05 LQLSYY123 pKa = 10.46 IGRR126 pKa = 11.84 ALPYY130 pKa = 10.29 GDD132 pKa = 4.04 ATICEE137 pKa = 4.48 HH138 pKa = 6.53 ALKK141 pKa = 10.66 SHH143 pKa = 6.7 ADD145 pKa = 3.47 VLGSLGVTPPSLLGAARR162 pKa = 11.84 GWAKK166 pKa = 10.22 EE167 pKa = 3.61 WAIKK171 pKa = 10.41 NRR173 pKa = 11.84 LSLDD177 pKa = 4.5 LIRR180 pKa = 11.84 EE181 pKa = 4.07 PPIQEE186 pKa = 4.21 SSCLEE191 pKa = 3.64 YY192 pKa = 10.69 SRR194 pKa = 11.84 RR195 pKa = 11.84 EE196 pKa = 3.74 GGYY199 pKa = 9.14 ARR201 pKa = 11.84 AIKK204 pKa = 10.05 EE205 pKa = 3.78 HH206 pKa = 5.53 TARR209 pKa = 11.84 CLTLPPSSVRR219 pKa = 11.84 HH220 pKa = 4.84 SMKK223 pKa = 10.33 EE224 pKa = 3.68 GTRR227 pKa = 11.84 ASNPGRR233 pKa = 11.84 VLKK236 pKa = 10.77 DD237 pKa = 3.05 LAFADD242 pKa = 4.43 LSNTARR248 pKa = 11.84 VVAVPEE254 pKa = 4.4 RR255 pKa = 11.84 GWKK258 pKa = 9.77 ARR260 pKa = 11.84 IVTAHH265 pKa = 6.52 AAARR269 pKa = 11.84 VAFLHH274 pKa = 5.63 SLRR277 pKa = 11.84 HH278 pKa = 5.23 GLFAALRR285 pKa = 11.84 NDD287 pKa = 3.01 RR288 pKa = 11.84 RR289 pKa = 11.84 TKK291 pKa = 10.35 LVAEE295 pKa = 4.53 GLHH298 pKa = 5.97 RR299 pKa = 11.84 EE300 pKa = 4.0 AVEE303 pKa = 3.92 ALFATGVDD311 pKa = 3.79 PRR313 pKa = 11.84 CTIVSADD320 pKa = 3.98 LSAASDD326 pKa = 3.8 TLHH329 pKa = 7.27 GDD331 pKa = 4.04 LLQAIVEE338 pKa = 4.33 GLGDD342 pKa = 4.41 ALGCTSKK349 pKa = 10.96 FMDD352 pKa = 4.24 EE353 pKa = 4.11 FTNAAVGSYY362 pKa = 8.87 TLSYY366 pKa = 10.3 PDD368 pKa = 4.05 GSTVTTQRR376 pKa = 11.84 GALMGLPTTWPLLCLTHH393 pKa = 7.07 LFWCDD398 pKa = 3.08 QSLVQLHH405 pKa = 6.09 GRR407 pKa = 11.84 GGCRR411 pKa = 11.84 EE412 pKa = 3.95 KK413 pKa = 10.13 EE414 pKa = 4.42 TPGSSRR420 pKa = 11.84 EE421 pKa = 4.1 AEE423 pKa = 4.62 VICGDD428 pKa = 4.27 DD429 pKa = 4.46 LAAAWRR435 pKa = 11.84 PDD437 pKa = 2.92 RR438 pKa = 11.84 VAAYY442 pKa = 9.93 EE443 pKa = 4.34 EE444 pKa = 4.33 IAVLCGAVFSPGKK457 pKa = 9.36 HH458 pKa = 5.8 LKK460 pKa = 10.23 SKK462 pKa = 10.57 VYY464 pKa = 10.63 GIFTEE469 pKa = 5.28 DD470 pKa = 3.17 IYY472 pKa = 11.43 SVQVSCVPTRR482 pKa = 11.84 VTRR485 pKa = 11.84 EE486 pKa = 3.69 VSKK489 pKa = 9.14 TWTGPVDD496 pKa = 4.03 KK497 pKa = 11.33 VPMNLVNEE505 pKa = 4.77 LGQLLRR511 pKa = 11.84 WRR513 pKa = 11.84 AQRR516 pKa = 11.84 YY517 pKa = 8.61 KK518 pKa = 10.66 VGKK521 pKa = 8.04 TGAFQFPPRR530 pKa = 11.84 NVTLSYY536 pKa = 10.72 VSKK539 pKa = 9.72 EE540 pKa = 4.18 TGLKK544 pKa = 8.59 MNPVSVLFRR553 pKa = 11.84 EE554 pKa = 4.35 WSTAIPLRR562 pKa = 11.84 WAVRR566 pKa = 11.84 APKK569 pKa = 9.47 HH570 pKa = 5.31 APGMRR575 pKa = 11.84 GDD577 pKa = 4.2 LPPWVTVPLAAHH589 pKa = 6.87 AVASAHH595 pKa = 6.33 PGRR598 pKa = 11.84 WSKK601 pKa = 10.25 VCRR604 pKa = 11.84 VVKK607 pKa = 10.5 LCYY610 pKa = 9.67 PGMAKK615 pKa = 10.21 FFASHH620 pKa = 7.38 GIPPYY625 pKa = 10.31 LPRR628 pKa = 11.84 VLGGGGLPTPLGDD641 pKa = 2.98 RR642 pKa = 11.84 VKK644 pKa = 10.26 IGRR647 pKa = 11.84 VASRR651 pKa = 11.84 RR652 pKa = 11.84 IRR654 pKa = 11.84 KK655 pKa = 8.95 ALGGALYY662 pKa = 10.49 RR663 pKa = 11.84 STDD666 pKa = 3.12 PTTFGSIWTTALSPAYY682 pKa = 10.15 ALALADD688 pKa = 4.35 AQDD691 pKa = 4.5 LDD693 pKa = 4.66 AMHH696 pKa = 7.6 PDD698 pKa = 3.26 AFKK701 pKa = 11.01 VRR703 pKa = 11.84 QTRR706 pKa = 11.84 SLRR709 pKa = 11.84 PPFKK713 pKa = 11.06 DD714 pKa = 2.81 NGNYY718 pKa = 10.35 ADD720 pKa = 4.1 FVEE723 pKa = 4.46 MRR725 pKa = 11.84 AGKK728 pKa = 10.38 GSLWALRR735 pKa = 11.84 QGAQLVGRR743 pKa = 11.84 SFTPSPARR751 pKa = 11.84 VSNALRR757 pKa = 11.84 TKK759 pKa = 9.92 VRR761 pKa = 11.84 KK762 pKa = 9.37 ALAKK766 pKa = 10.0 RR767 pKa = 11.84 GYY769 pKa = 9.25 LRR771 pKa = 11.84 SSAPVSKK778 pKa = 10.46 LIEE781 pKa = 4.25 RR782 pKa = 11.84 ASVKK786 pKa = 10.32 PSDD789 pKa = 3.7 HH790 pKa = 4.59 MWIRR794 pKa = 11.84 EE795 pKa = 3.95 RR796 pKa = 11.84 PVLSFGRR803 pKa = 11.84 FGGRR807 pKa = 11.84 MSGKK811 pKa = 9.82 KK812 pKa = 9.45 EE813 pKa = 4.0 EE814 pKa = 4.28 PVSFQWKK821 pKa = 9.09 KK822 pKa = 10.44 RR823 pKa = 11.84 EE824 pKa = 4.14 SVKK827 pKa = 10.32 TGSS830 pKa = 3.2
Molecular weight: 91.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.289
IPC2_protein 9.414
IPC_protein 9.677
Toseland 10.379
ProMoST 9.984
Dawson 10.496
Bjellqvist 10.175
Wikipedia 10.657
Rodwell 10.833
Grimsley 10.555
Solomon 10.555
Lehninger 10.526
Nozaki 10.394
DTASelect 10.16
Thurlkill 10.379
EMBOSS 10.774
Sillero 10.423
Patrickios 10.496
IPC_peptide 10.555
IPC2_peptide 9.107
IPC2.peptide.svr19 8.475
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
830
830
830
830.0
91.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.325 ± 0.0
1.928 ± 0.0
3.976 ± 0.0
4.217 ± 0.0
3.373 ± 0.0
7.831 ± 0.0
2.53 ± 0.0
2.771 ± 0.0
5.181 ± 0.0
10.0 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.566 ± 0.0
1.807 ± 0.0
6.265 ± 0.0
2.169 ± 0.0
9.398 ± 0.0
7.47 ± 0.0
6.024 ± 0.0
7.711 ± 0.0
2.41 ± 0.0
2.048 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here