Fimbriiglobus ruber
Average proteome isoelectric point is 6.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10212 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A225E089|A0A225E089_9BACT Peptidase_M78 domain-containing protein OS=Fimbriiglobus ruber OX=1908690 GN=FRUB_00661 PE=4 SV=1
MM1 pKa = 7.54 IRR3 pKa = 11.84 NDD5 pKa = 3.57 WRR7 pKa = 11.84 RR8 pKa = 11.84 MIQRR12 pKa = 11.84 VGRR15 pKa = 11.84 GQHH18 pKa = 5.11 KK19 pKa = 9.16 QNRR22 pKa = 11.84 SPSMTVLQLEE32 pKa = 4.55 DD33 pKa = 3.73 RR34 pKa = 11.84 SVPATFTAGSIQGQDD49 pKa = 2.62 GWTGGTAAISTNVVQTVDD67 pKa = 3.3 QSGANAFSGTGAWLVSNSTANGGYY91 pKa = 9.92 NGNFVGWPFSPGLSVTAGDD110 pKa = 4.01 PSSGAGADD118 pKa = 3.43 TFTATLYY125 pKa = 10.54 FKK127 pKa = 10.47 SASSAADD134 pKa = 3.46 GSNIEE139 pKa = 4.09 VDD141 pKa = 3.75 LGSAAGDD148 pKa = 3.45 DD149 pKa = 3.66 RR150 pKa = 11.84 TNFLALTNEE159 pKa = 4.1 DD160 pKa = 4.23 DD161 pKa = 4.84 ANGGLQIRR169 pKa = 11.84 AAEE172 pKa = 4.12 PMTDD176 pKa = 3.04 GTTNFYY182 pKa = 8.51 PTVSIATDD190 pKa = 3.18 ITRR193 pKa = 11.84 ATYY196 pKa = 10.22 HH197 pKa = 6.61 RR198 pKa = 11.84 LDD200 pKa = 3.29 VTANYY205 pKa = 11.0 ADD207 pKa = 3.86 GTDD210 pKa = 3.27 NDD212 pKa = 4.53 TFTVSLDD219 pKa = 3.45 GKK221 pKa = 10.48 VLTNPRR227 pKa = 11.84 TGGTTFGTFEE237 pKa = 4.49 AYY239 pKa = 8.47 EE240 pKa = 4.22 AAASPPASPPNYY252 pKa = 8.69 EE253 pKa = 3.74 QTSRR257 pKa = 11.84 LLFRR261 pKa = 11.84 SGTAPSGFDD270 pKa = 3.22 ALTSPGEE277 pKa = 4.08 FSDD280 pKa = 3.66 TGAQGFYY287 pKa = 10.19 FDD289 pKa = 3.62 NVSYY293 pKa = 10.97 RR294 pKa = 11.84 DD295 pKa = 3.89 YY296 pKa = 11.12 NQSDD300 pKa = 3.76 PSATLASYY308 pKa = 10.55 AATFEE313 pKa = 4.25 TQPAVTYY320 pKa = 10.52 ANPAWSSLATGDD332 pKa = 5.02 PITDD336 pKa = 4.28 ADD338 pKa = 4.47 PLTAGDD344 pKa = 3.76 QPATYY349 pKa = 9.84 GVNAFATITDD359 pKa = 3.53 AVTAVAAGGTVIANAGTYY377 pKa = 10.26 AEE379 pKa = 4.44 NVTINKK385 pKa = 9.24 PLTLEE390 pKa = 4.52 GQSSTNTVIEE400 pKa = 4.46 PGLTSSYY407 pKa = 9.75 NTDD410 pKa = 2.93 NVVTVLANNVTVEE423 pKa = 3.99 GFTIQGSITGTPPVGQSSGFTLSSGTTVYY452 pKa = 10.84 AAAGISNSADD462 pKa = 3.36 VTSTATDD469 pKa = 3.1 ISGLTVQNNVIKK481 pKa = 10.73 DD482 pKa = 3.56 FTQFGVDD489 pKa = 3.46 GDD491 pKa = 4.2 TSSGAVSTGNTIANNVIQDD510 pKa = 3.52 IPEE513 pKa = 4.31 NNPGSGFYY521 pKa = 10.62 GQGVVVADD529 pKa = 3.6 NFYY532 pKa = 11.3 AAITGNTITNVRR544 pKa = 11.84 TGIQTDD550 pKa = 3.37 NNYY553 pKa = 9.63 TSAGAFAPSISNNIVSATVKK573 pKa = 10.64 GIYY576 pKa = 10.46 LNLQYY581 pKa = 11.07 SAASPFAITGNTITQADD598 pKa = 4.31 GTVSPAYY605 pKa = 10.28 NVGLLVQSIYY615 pKa = 11.42 NGVTTNIQGNNVSGFLYY632 pKa = 10.2 GVEE635 pKa = 4.11 FAGNSATTPVEE646 pKa = 4.19 VQGGTLSGNTYY657 pKa = 10.28 GVWATNNDD665 pKa = 3.2 YY666 pKa = 10.72 FYY668 pKa = 10.24 PANFNTTAALSGVAITDD685 pKa = 3.67 SKK687 pKa = 9.86 TAAIWVDD694 pKa = 3.41 STSQSMAGTSDD705 pKa = 3.2 TTDD708 pKa = 3.24 TVSLAVGGGTTVTGGPTGLLIAGSLSNITGNALGTVSFAGVSGDD752 pKa = 5.36 DD753 pKa = 3.12 ITLAAGALVGQTLDD767 pKa = 3.57 ATAATYY773 pKa = 10.82 DD774 pKa = 3.97 GVVGTAATPTQGYY787 pKa = 10.22 AIADD791 pKa = 3.85 KK792 pKa = 10.37 VTDD795 pKa = 4.34 YY796 pKa = 11.23 IDD798 pKa = 3.73 NPALGFVRR806 pKa = 11.84 ITPATVYY813 pKa = 7.48 VTPKK817 pKa = 10.35 SEE819 pKa = 4.11 STTAGAVQRR828 pKa = 11.84 GVTAAANNDD837 pKa = 3.89 TVDD840 pKa = 3.63 VAAGTYY846 pKa = 10.33 AEE848 pKa = 4.49 NVTINKK854 pKa = 9.25 PLTLEE859 pKa = 4.6 GEE861 pKa = 4.41 SSANTVIEE869 pKa = 4.41 PGLTSSYY876 pKa = 9.75 NTDD879 pKa = 2.93 NVVTVLANDD888 pKa = 3.39 VTVEE892 pKa = 3.95 GFTIQGSITGTPPVGQSSGFTLSSGTTVYY921 pKa = 10.84 AAAGISNSADD931 pKa = 3.36 VTSTATDD938 pKa = 3.1 ISGLTVQNNVIKK950 pKa = 10.73 DD951 pKa = 3.56 FTQFGVDD958 pKa = 3.46 GDD960 pKa = 4.2 TSSGAVSTGNTIANNVIQDD979 pKa = 3.52 IPEE982 pKa = 4.31 NNPGSGFYY990 pKa = 10.62 GQGVVVADD998 pKa = 3.6 NFYY1001 pKa = 11.3 AAITGNTITNVRR1013 pKa = 11.84 TGIQTDD1019 pKa = 3.37 NNYY1022 pKa = 9.63 TSAGAFAPSISNNIVSATVKK1042 pKa = 10.64 GIYY1045 pKa = 10.46 LNLQYY1050 pKa = 11.07 SAASPFAITGNTITQADD1067 pKa = 4.31 GTVSPAYY1074 pKa = 10.28 NVGLLVQSIYY1084 pKa = 11.42 NGVTTNIQGNNVSGFLYY1101 pKa = 10.2 GVEE1104 pKa = 4.11 FAGNSATTPVEE1115 pKa = 4.19 VQGGTLSGNTYY1126 pKa = 10.28 GVWATNNDD1134 pKa = 3.2 YY1135 pKa = 10.72 FYY1137 pKa = 10.24 PANFNTTAALSGVAITDD1154 pKa = 3.67 SKK1156 pKa = 9.86 TAAIWVDD1163 pKa = 3.41 STSQSMAGTSDD1174 pKa = 3.25 TTDD1177 pKa = 3.14 SVSLAVGGGTSVTGGPTGLLIAGGLSNITGNALGTVSFAGVTGDD1221 pKa = 5.59 DD1222 pKa = 3.15 ITLAAGALAGQTLDD1236 pKa = 3.42 ATGVTFNGTTGSTATPAQDD1255 pKa = 3.29 YY1256 pKa = 11.03 AIADD1260 pKa = 4.37 KK1261 pKa = 10.14 ITDD1264 pKa = 4.81 FIDD1267 pKa = 4.46 DD1268 pKa = 3.79 PTLGFVQITPATVYY1282 pKa = 7.54 VTPKK1286 pKa = 10.46 SEE1288 pKa = 3.9 ATTAGAIPRR1297 pKa = 11.84 AVTVASAGDD1306 pKa = 4.1 TIDD1309 pKa = 3.61 VAAGTYY1315 pKa = 8.49 TGNIDD1320 pKa = 3.3 LSKK1323 pKa = 10.45 NVSVRR1328 pKa = 11.84 IDD1330 pKa = 3.71 GPSSLTGAISGTTGSITDD1348 pKa = 3.52 TLGAVSLGDD1357 pKa = 3.57 GSAAGVATSSTLDD1370 pKa = 3.37 VNGQTVTLLDD1380 pKa = 3.75 SDD1382 pKa = 4.58 GADD1385 pKa = 3.69 LDD1387 pKa = 4.53 GEE1389 pKa = 4.41 TDD1391 pKa = 3.66 LVGGTLVSANGINVGGTLAGPGTVGAATIQAGGNVEE1427 pKa = 4.24 PGGSGTGILNTGNIAFAAGATLTPTLKK1454 pKa = 9.9 GTTAGTTYY1462 pKa = 10.91 DD1463 pKa = 3.47 QVNTTGTVDD1472 pKa = 4.3 LGGATLNLNLAFTPAAGDD1490 pKa = 3.28 TFTIVNNDD1498 pKa = 2.7 GTDD1501 pKa = 3.64 PVVGTFAGLPEE1512 pKa = 4.24 GSVFKK1517 pKa = 11.01 VGSQFFQISYY1527 pKa = 10.53 KK1528 pKa = 10.89 GGDD1531 pKa = 3.81 GNDD1534 pKa = 3.38 VTLKK1538 pKa = 10.55 AVPSSVLISAPSAAFANDD1556 pKa = 3.26 TSTITYY1562 pKa = 7.21 TVTYY1566 pKa = 9.73 ADD1568 pKa = 4.3 PNFASSTLSSGDD1580 pKa = 3.15 VTLNTTGTAAVGSVVVSGTGATRR1603 pKa = 11.84 TVTLSNITGDD1613 pKa = 3.74 GAVGISLPAGTGTDD1627 pKa = 3.22 TSSNPEE1633 pKa = 4.01 PGAGPSATFTADD1645 pKa = 2.85 STAPTVAIGAPSAAFANGTSTVTYY1669 pKa = 9.06 TITYY1673 pKa = 8.23 TDD1675 pKa = 3.45 TNFADD1680 pKa = 3.84 STLTPGDD1687 pKa = 3.39 VTLNATGDD1695 pKa = 3.65 ANATISIDD1703 pKa = 3.43 SGTGATRR1710 pKa = 11.84 TVTLTHH1716 pKa = 5.09 VTGTGTLGISLLADD1730 pKa = 3.48 TAVDD1734 pKa = 3.55 SAGNEE1739 pKa = 3.75 APAAGPSGTFTADD1752 pKa = 2.97 TTNPTVVIGAPSAATANAGSTITYY1776 pKa = 7.58 TITYY1780 pKa = 9.67 SDD1782 pKa = 3.81 TNFDD1786 pKa = 3.74 QSTLTAADD1794 pKa = 3.39 VTLNTTGTASATVTVDD1810 pKa = 3.2 GGAGATRR1817 pKa = 11.84 TVTLTHH1823 pKa = 5.96 ITGDD1827 pKa = 3.47 GTLGISLAAGTAHH1840 pKa = 7.56 DD1841 pKa = 4.43 LAGNEE1846 pKa = 4.27 APVAGPSGTFTVDD1859 pKa = 2.87 TTAPTVAIGAPSAALANAASTVTFTVTYY1887 pKa = 10.89 ADD1889 pKa = 3.74 ANFAASTLTAADD1901 pKa = 3.43 VTLNTTGTAAGTVSVSGSGTTWTVTVTGITGDD1933 pKa = 3.53 GTLGISLAAGTASDD1947 pKa = 3.8 TAGNLAPAAGPSATFTVDD1965 pKa = 2.97 DD1966 pKa = 4.05 TAPTVSISPPSVTVANGSSTVTYY1989 pKa = 9.33 TVTYY1993 pKa = 10.78 ADD1995 pKa = 4.39 ANFADD2000 pKa = 4.28 STLTAADD2007 pKa = 3.44 VTLNATGTASATVSVDD2023 pKa = 3.01 SGSGTTRR2030 pKa = 11.84 TVTLTHH2036 pKa = 5.96 ITGDD2040 pKa = 3.47 GTLGISLAAGTAHH2053 pKa = 7.11 DD2054 pKa = 4.52 QLSDD2058 pKa = 3.73 PAPAAGPSGTFAVDD2072 pKa = 3.45 TTAPTVAIGNPSVAITNGSSTVTYY2096 pKa = 9.6 TITYY2100 pKa = 10.04 SDD2102 pKa = 4.03 ANFAASTLTAADD2114 pKa = 3.43 VTLNTTGTATATVSVDD2130 pKa = 2.9 SGTGTTRR2137 pKa = 11.84 TITLSNITGDD2147 pKa = 3.48 GTLGISLVAGTASDD2161 pKa = 3.48 AAEE2164 pKa = 4.33 NLAPAAGPSGTFAVDD2179 pKa = 3.45 TTAPTVTISSPAVTGAGAAEE2199 pKa = 4.23 VVTYY2203 pKa = 9.3 TITYY2207 pKa = 10.34 ADD2209 pKa = 4.01 ANFADD2214 pKa = 4.28 STLTAADD2221 pKa = 3.44 VTLNATGGTTASVAVSGTGTTRR2243 pKa = 11.84 TVTLTHH2249 pKa = 6.03 ITGSGTVGISLVSGTAVDD2267 pKa = 3.61 TAGNLAPAAGPSATAAAVPVDD2288 pKa = 4.07 ANAVGQFAASPDD2300 pKa = 3.59 AGGSGIVTVYY2310 pKa = 10.75 NADD2313 pKa = 3.16 GSVAYY2318 pKa = 8.44 TVNPFPGVGAQTGVRR2333 pKa = 11.84 AVMAGVTGSATPDD2346 pKa = 3.43 VIAGTGPGVRR2356 pKa = 11.84 AQVVVVSGTTHH2367 pKa = 5.92 AVVMTLNPFEE2377 pKa = 5.56 DD2378 pKa = 4.21 SFTGGVFVAAADD2390 pKa = 4.12 LNGDD2394 pKa = 3.6 GHH2396 pKa = 8.59 ADD2398 pKa = 2.99 IVVSPDD2404 pKa = 2.88 VGGGGRR2410 pKa = 11.84 ITVYY2414 pKa = 10.72 DD2415 pKa = 3.89 GATGQVIANFFGIDD2429 pKa = 3.37 DD2430 pKa = 3.83 PAFRR2434 pKa = 11.84 GGARR2438 pKa = 11.84 VSFGDD2443 pKa = 3.56 VNGDD2447 pKa = 3.63 GVPDD2451 pKa = 4.93 LIVSAGTGGGPRR2463 pKa = 11.84 VAIFDD2468 pKa = 3.69 GRR2470 pKa = 11.84 SIGLGKK2476 pKa = 8.74 TPTKK2480 pKa = 10.53 LVADD2484 pKa = 5.11 FFAFEE2489 pKa = 3.85 PSLRR2493 pKa = 11.84 NGAYY2497 pKa = 9.55 VAAGDD2502 pKa = 4.18 FNGDD2506 pKa = 3.09 GYY2508 pKa = 11.81 ADD2510 pKa = 4.2 LVAGGGPDD2518 pKa = 3.31 GAPRR2522 pKa = 11.84 VEE2524 pKa = 4.3 VLDD2527 pKa = 4.25 GASLLSSNGQAPTMVANFFAGDD2549 pKa = 3.42 PSLRR2553 pKa = 11.84 SGARR2557 pKa = 11.84 VAVKK2561 pKa = 10.56 NLDD2564 pKa = 3.58 GDD2566 pKa = 4.11 GSADD2570 pKa = 3.72 LVVGLATGTGTQVATYY2586 pKa = 10.0 LGKK2589 pKa = 10.33 DD2590 pKa = 3.41 LTASGTPTAAAEE2602 pKa = 4.1 LDD2604 pKa = 3.93 PFPGFTGGVFVGG2616 pKa = 4.17
Molecular weight: 257.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.676
IPC2_protein 3.605
IPC_protein 3.681
Toseland 3.427
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.656
Rodwell 3.49
Grimsley 3.325
Solomon 3.694
Lehninger 3.656
Nozaki 3.795
DTASelect 4.126
Thurlkill 3.49
EMBOSS 3.668
Sillero 3.808
Patrickios 1.074
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.729
Protein with the highest isoelectric point:
>tr|A0A225DK67|A0A225DK67_9BACT Zinc protease OS=Fimbriiglobus ruber OX=1908690 GN=FRUB_06709 PE=4 SV=1
MM1 pKa = 7.5 SFPRR5 pKa = 11.84 KK6 pKa = 8.37 FTGNLSVRR14 pKa = 11.84 PVRR17 pKa = 11.84 RR18 pKa = 11.84 PAPGPRR24 pKa = 11.84 WRR26 pKa = 11.84 HH27 pKa = 4.8 CWVTSWLGTGADD39 pKa = 3.47 SRR41 pKa = 11.84 RR42 pKa = 11.84 VRR44 pKa = 11.84 TRR46 pKa = 11.84 RR47 pKa = 11.84 PRR49 pKa = 11.84 GTKK52 pKa = 10.06 SPTALRR58 pKa = 11.84 GSARR62 pKa = 11.84 GNRR65 pKa = 11.84 RR66 pKa = 11.84 TARR69 pKa = 11.84 PRR71 pKa = 11.84 ASPAARR77 pKa = 11.84 FVRR80 pKa = 11.84 GGWW83 pKa = 3.16
Molecular weight: 9.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.453
IPC2_protein 10.979
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.232
Rodwell 12.296
Grimsley 12.793
Solomon 13.261
Lehninger 13.159
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.261
Sillero 12.749
Patrickios 12.018
IPC_peptide 13.261
IPC2_peptide 12.252
IPC2.peptide.svr19 9.145
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10212
0
10212
3250331
37
8355
318.3
34.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.72 ± 0.04
0.981 ± 0.009
5.925 ± 0.02
5.172 ± 0.028
3.558 ± 0.017
8.793 ± 0.043
2.015 ± 0.015
3.848 ± 0.019
3.852 ± 0.032
9.365 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.712 ± 0.013
2.801 ± 0.024
6.283 ± 0.025
3.08 ± 0.016
7.132 ± 0.046
5.17 ± 0.036
6.565 ± 0.062
8.092 ± 0.024
1.524 ± 0.012
2.412 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here