Klebsiella phage KPP5665-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A286S2C1|A0A286S2C1_9CAUD XRE family transcriptional regulator OS=Klebsiella phage KPP5665-2 OX=2034166 GN=KPP56652_38 PE=4 SV=1
MM1 pKa = 7.9SNGQHH6 pKa = 5.61YY7 pKa = 10.2AYY9 pKa = 9.12PNPSNATPGGMTYY22 pKa = 10.25RR23 pKa = 11.84QYY25 pKa = 11.41LIAKK29 pKa = 7.75IAPVMITNFFSNDD42 pKa = 2.17AWTDD46 pKa = 3.37YY47 pKa = 11.74DD48 pKa = 4.25DD49 pKa = 4.75LARR52 pKa = 11.84TLMMAVDD59 pKa = 5.04AIIEE63 pKa = 4.31AEE65 pKa = 4.11QEE67 pKa = 3.95TAEE70 pKa = 4.09

Molecular weight: 7.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A286S1P8|A0A286S1P8_9CAUD Uncharacterized protein OS=Klebsiella phage KPP5665-2 OX=2034166 GN=KPP56652_35 PE=4 SV=1
MM1 pKa = 7.77LIGYY5 pKa = 9.56ARR7 pKa = 11.84VSTGDD12 pKa = 3.42QNLDD16 pKa = 3.43LQKK19 pKa = 10.93NALIRR24 pKa = 11.84AEE26 pKa = 4.18CEE28 pKa = 3.96LVFEE32 pKa = 5.43DD33 pKa = 4.04MASGKK38 pKa = 8.15NARR41 pKa = 11.84RR42 pKa = 11.84PGLKK46 pKa = 9.54RR47 pKa = 11.84ALRR50 pKa = 11.84RR51 pKa = 11.84LRR53 pKa = 11.84PGDD56 pKa = 3.56VLVVWKK62 pKa = 10.33LDD64 pKa = 3.43RR65 pKa = 11.84LGRR68 pKa = 11.84SVRR71 pKa = 11.84DD72 pKa = 3.94LITLVSEE79 pKa = 4.16LQARR83 pKa = 11.84GVNFRR88 pKa = 11.84SLTDD92 pKa = 4.13SIDD95 pKa = 3.23TSTPAGRR102 pKa = 11.84FFFHH106 pKa = 6.71VMSALAEE113 pKa = 4.12MEE115 pKa = 3.97RR116 pKa = 11.84EE117 pKa = 4.21LIVEE121 pKa = 4.57RR122 pKa = 11.84TRR124 pKa = 11.84AGLAAARR131 pKa = 11.84EE132 pKa = 4.15QGRR135 pKa = 11.84VGGRR139 pKa = 11.84RR140 pKa = 11.84RR141 pKa = 11.84VMTEE145 pKa = 3.59EE146 pKa = 3.72VVEE149 pKa = 4.22RR150 pKa = 11.84CRR152 pKa = 11.84RR153 pKa = 11.84MLEE156 pKa = 3.94NGATRR161 pKa = 11.84QQIADD166 pKa = 3.81VIGVNVKK173 pKa = 8.2TLYY176 pKa = 10.32KK177 pKa = 9.81YY178 pKa = 10.94LPAAVV183 pKa = 3.68

Molecular weight: 20.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

12005

37

1220

210.6

23.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.921 ± 0.377

1.058 ± 0.147

5.781 ± 0.217

5.773 ± 0.365

3.39 ± 0.167

7.597 ± 0.27

1.641 ± 0.173

5.639 ± 0.242

4.923 ± 0.424

8.621 ± 0.248

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.682 ± 0.14

4.406 ± 0.243

4.24 ± 0.271

3.99 ± 0.289

5.889 ± 0.394

6.914 ± 0.38

6.297 ± 0.302

6.414 ± 0.307

1.616 ± 0.116

3.207 ± 0.25
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski