Gammapapillomavirus 23
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D2AM71|A0A2D2AM71_9PAPI Protein E7 OS=Gammapapillomavirus 23 OX=1961680 GN=E7 PE=3 SV=1
MM1 pKa = 7.45 TSLKK5 pKa = 9.93 RR6 pKa = 11.84 RR7 pKa = 11.84 KK8 pKa = 9.55 RR9 pKa = 11.84 DD10 pKa = 3.47 TVDD13 pKa = 2.81 NLYY16 pKa = 10.32 RR17 pKa = 11.84 QCQLGADD24 pKa = 4.28 CPPDD28 pKa = 3.29 VRR30 pKa = 11.84 NKK32 pKa = 10.25 VEE34 pKa = 4.05 GTTLADD40 pKa = 3.71 KK41 pKa = 10.79 LLQAFGSIIFLGGLGIGSGSGSGAVTAGRR70 pKa = 11.84 TIPDD74 pKa = 4.56 LAPDD78 pKa = 4.12 ATPIRR83 pKa = 11.84 PTRR86 pKa = 11.84 PLRR89 pKa = 11.84 PSSGPNTTRR98 pKa = 11.84 PFSVPIDD105 pKa = 4.0 RR106 pKa = 11.84 IGVPGAGGRR115 pKa = 11.84 PVTIDD120 pKa = 3.23 ATSSSIVPLTEE131 pKa = 4.07 AVPDD135 pKa = 3.96 TVITLGDD142 pKa = 3.53 PTIGVTTDD150 pKa = 2.68 IAVDD154 pKa = 3.46 VNPIEE159 pKa = 5.26 LEE161 pKa = 4.58 PINQGPDD168 pKa = 2.88 TPAIINVTPIEE179 pKa = 4.35 PPPVRR184 pKa = 11.84 ILYY187 pKa = 10.48 SDD189 pKa = 3.47 TTTYY193 pKa = 11.18 APPEE197 pKa = 4.08 TVQIAFTDD205 pKa = 3.67 PNLNVFVDD213 pKa = 4.25 PASTGQHH220 pKa = 5.78 IGLEE224 pKa = 4.38 EE225 pKa = 4.72 IEE227 pKa = 5.14 LDD229 pKa = 4.06 TIGGPSTFEE238 pKa = 3.8 IEE240 pKa = 4.02 EE241 pKa = 4.24 AAPTTSTPLEE251 pKa = 4.14 RR252 pKa = 11.84 LGQVYY257 pKa = 10.23 NRR259 pKa = 11.84 ARR261 pKa = 11.84 QYY263 pKa = 10.24 YY264 pKa = 7.78 NRR266 pKa = 11.84 RR267 pKa = 11.84 VQQVPTRR274 pKa = 11.84 NIDD277 pKa = 3.57 FLGQPSRR284 pKa = 11.84 AVLFGFEE291 pKa = 4.34 NPAFLDD297 pKa = 3.85 DD298 pKa = 3.63 VTLEE302 pKa = 4.05 FEE304 pKa = 3.78 QDD306 pKa = 3.37 LRR308 pKa = 11.84 EE309 pKa = 4.16 VAAAPDD315 pKa = 3.44 EE316 pKa = 4.38 DD317 pKa = 4.16 FRR319 pKa = 11.84 DD320 pKa = 3.64 VRR322 pKa = 11.84 QLHH325 pKa = 6.26 RR326 pKa = 11.84 PTFSITDD333 pKa = 3.3 EE334 pKa = 4.08 GLIRR338 pKa = 11.84 LSRR341 pKa = 11.84 LGSRR345 pKa = 11.84 ATMQTRR351 pKa = 11.84 SGRR354 pKa = 11.84 VFGSDD359 pKa = 2.15 AHH361 pKa = 6.41 FYY363 pKa = 10.68 YY364 pKa = 10.54 DD365 pKa = 3.26 VSEE368 pKa = 4.21 IPEE371 pKa = 4.17 AGEE374 pKa = 4.92 IEE376 pKa = 4.37 MQDD379 pKa = 3.48 LVTPGVPYY387 pKa = 10.24 TIVDD391 pKa = 3.9 PQAEE395 pKa = 4.37 SSFVNALADD404 pKa = 3.48 VTLFTEE410 pKa = 4.41 DD411 pKa = 3.7 QLVDD415 pKa = 4.23 FYY417 pKa = 12.02 NEE419 pKa = 4.2 SFDD422 pKa = 3.73 NAQLILEE429 pKa = 4.61 GQTEE433 pKa = 4.16 AEE435 pKa = 4.0 EE436 pKa = 5.56 RR437 pKa = 11.84 YY438 pKa = 7.83 TFPIPISTTAIKK450 pKa = 10.37 PFVVDD455 pKa = 3.3 IGNGIFYY462 pKa = 10.49 SSPTQISTNNYY473 pKa = 8.06 SPKK476 pKa = 10.08 FPFIPVSPGVTINMYY491 pKa = 10.83 SDD493 pKa = 5.37 DD494 pKa = 4.42 YY495 pKa = 11.78 YY496 pKa = 11.61 LDD498 pKa = 4.28 PSLLKK503 pKa = 10.39 RR504 pKa = 11.84 KK505 pKa = 9.64 RR506 pKa = 11.84 KK507 pKa = 10.04 RR508 pKa = 11.84 SDD510 pKa = 3.14 DD511 pKa = 3.61 FF512 pKa = 6.03
Molecular weight: 56.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.502
IPC2_protein 4.393
IPC_protein 4.38
Toseland 4.19
ProMoST 4.495
Dawson 4.342
Bjellqvist 4.495
Wikipedia 4.24
Rodwell 4.202
Grimsley 4.101
Solomon 4.342
Lehninger 4.291
Nozaki 4.444
DTASelect 4.66
Thurlkill 4.202
EMBOSS 4.253
Sillero 4.495
Patrickios 3.935
IPC_peptide 4.342
IPC2_peptide 4.469
IPC2.peptide.svr19 4.444
Protein with the highest isoelectric point:
>tr|A0A2D2AM70|A0A2D2AM70_9PAPI Minor capsid protein L2 OS=Gammapapillomavirus 23 OX=1961680 GN=L2 PE=3 SV=1
MM1 pKa = 7.03 NQADD5 pKa = 4.0 LTSRR9 pKa = 11.84 SDD11 pKa = 3.46 ALQEE15 pKa = 3.93 RR16 pKa = 11.84 LMNLYY21 pKa = 10.43 EE22 pKa = 4.63 SGAKK26 pKa = 8.78 TLEE29 pKa = 3.77 AQIEE33 pKa = 4.15 HH34 pKa = 5.96 WQLVRR39 pKa = 11.84 KK40 pKa = 10.11 LNVLYY45 pKa = 10.7 YY46 pKa = 8.8 YY47 pKa = 10.76 ARR49 pKa = 11.84 KK50 pKa = 9.81 EE51 pKa = 4.21 KK52 pKa = 10.35 FSHH55 pKa = 7.04 LGLQPLPSLTVSEE68 pKa = 4.7 YY69 pKa = 10.45 KK70 pKa = 10.56 SKK72 pKa = 10.8 EE73 pKa = 3.76 AIQIVLLLRR82 pKa = 11.84 SLQNSPYY89 pKa = 10.83 AEE91 pKa = 4.3 EE92 pKa = 4.17 EE93 pKa = 4.25 WNLSDD98 pKa = 3.83 TSAEE102 pKa = 4.53 LIHH105 pKa = 6.36 TPPKK109 pKa = 9.49 NTFKK113 pKa = 10.76 KK114 pKa = 9.68 GPYY117 pKa = 9.47 RR118 pKa = 11.84 VDD120 pKa = 2.16 VWFDD124 pKa = 3.65 HH125 pKa = 6.58 NPQNSFPYY133 pKa = 9.31 TNYY136 pKa = 10.99 DD137 pKa = 3.9 FIYY140 pKa = 9.39 YY141 pKa = 9.64 QDD143 pKa = 4.78 YY144 pKa = 11.12 DD145 pKa = 4.26 EE146 pKa = 4.82 QWHH149 pKa = 5.54 KK150 pKa = 9.39 TAGLVDD156 pKa = 3.44 INGFYY161 pKa = 10.96 YY162 pKa = 10.39 EE163 pKa = 4.76 EE164 pKa = 4.72 PNGDD168 pKa = 3.02 RR169 pKa = 11.84 VYY171 pKa = 11.39 YY172 pKa = 10.1 FLFEE176 pKa = 4.43 GDD178 pKa = 3.05 AARR181 pKa = 11.84 YY182 pKa = 10.03 GEE184 pKa = 4.22 TGQWTVQFKK193 pKa = 9.07 NTTLSTSIPSSHH205 pKa = 6.75 RR206 pKa = 11.84 PQSTISPQGSISSSSDD222 pKa = 3.34 SISPTQSFNSKK233 pKa = 9.64 HH234 pKa = 5.75 SRR236 pKa = 11.84 SHH238 pKa = 5.91 EE239 pKa = 4.2 SEE241 pKa = 4.05 EE242 pKa = 4.62 GNASSSTGTPPQTPIRR258 pKa = 11.84 QRR260 pKa = 11.84 RR261 pKa = 11.84 RR262 pKa = 11.84 RR263 pKa = 11.84 RR264 pKa = 11.84 EE265 pKa = 4.02 GEE267 pKa = 3.9 PTSTTGEE274 pKa = 3.84 GSRR277 pKa = 11.84 AKK279 pKa = 10.06 RR280 pKa = 11.84 RR281 pKa = 11.84 RR282 pKa = 11.84 GGHH285 pKa = 4.73 TPVVRR290 pKa = 11.84 AGVSAAEE297 pKa = 3.99 VGSRR301 pKa = 11.84 HH302 pKa = 6.07 RR303 pKa = 11.84 GVPTSGLTRR312 pKa = 11.84 LARR315 pKa = 11.84 LEE317 pKa = 4.14 AEE319 pKa = 4.35 ARR321 pKa = 11.84 DD322 pKa = 3.73 PLILIFKK329 pKa = 9.89 GRR331 pKa = 11.84 SNQLKK336 pKa = 8.03 CWRR339 pKa = 11.84 YY340 pKa = 9.77 RR341 pKa = 11.84 LPKK344 pKa = 10.45 DD345 pKa = 3.46 SFNVATTVFRR355 pKa = 11.84 WAGEE359 pKa = 4.19 EE360 pKa = 4.02 EE361 pKa = 4.19 DD362 pKa = 5.68 DD363 pKa = 4.75 SYY365 pKa = 11.78 LSHH368 pKa = 7.28 RR369 pKa = 11.84 MLVAFNNQTQRR380 pKa = 11.84 KK381 pKa = 8.19 YY382 pKa = 10.12 FLKK385 pKa = 10.47 SVSIPRR391 pKa = 11.84 GVSFAYY397 pKa = 10.13 GQLDD401 pKa = 3.52 SLL403 pKa = 4.56
Molecular weight: 46.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.551
IPC2_protein 8.609
IPC_protein 8.609
Toseland 8.96
ProMoST 8.902
Dawson 9.326
Bjellqvist 9.107
Wikipedia 9.56
Rodwell 9.399
Grimsley 9.428
Solomon 9.385
Lehninger 9.326
Nozaki 8.99
DTASelect 9.092
Thurlkill 9.121
EMBOSS 9.399
Sillero 9.253
Patrickios 4.469
IPC_peptide 9.37
IPC2_peptide 7.775
IPC2.peptide.svr19 7.734
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2433
97
604
347.6
39.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.425 ± 0.358
2.261 ± 0.829
6.535 ± 0.528
6.494 ± 0.296
4.809 ± 0.551
5.302 ± 0.767
1.644 ± 0.302
5.179 ± 0.593
5.014 ± 0.889
9.042 ± 0.601
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.808 ± 0.348
5.302 ± 0.567
6.247 ± 1.053
4.316 ± 0.414
6.042 ± 0.671
6.987 ± 0.819
6.864 ± 0.598
5.59 ± 0.443
1.315 ± 0.34
3.822 ± 0.421
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here