Streptococcus satellite phage Javan251

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZMH6|A0A4D5ZMH6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan251 OX=2558592 GN=JavanS251_0010 PE=4 SV=1
MM1 pKa = 7.54VEE3 pKa = 4.1QKK5 pKa = 10.85EE6 pKa = 4.12DD7 pKa = 3.61LATVTQSLVNTEE19 pKa = 3.94MTVLNLLRR27 pKa = 11.84EE28 pKa = 4.29LNSLGVTGFNEE39 pKa = 3.6NALPDD44 pKa = 3.68NVKK47 pKa = 10.5RR48 pKa = 11.84LVEE51 pKa = 3.98ILDD54 pKa = 3.28IDD56 pKa = 4.37GLYY59 pKa = 10.6KK60 pKa = 10.76DD61 pKa = 4.82IFTEE65 pKa = 4.25SS66 pKa = 3.04

Molecular weight: 7.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZHY7|A0A4D5ZHY7_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan251 OX=2558592 GN=JavanS251_0011 PE=4 SV=1
MM1 pKa = 8.05KK2 pKa = 10.06IRR4 pKa = 11.84EE5 pKa = 4.24YY6 pKa = 9.41KK7 pKa = 8.92TKK9 pKa = 10.59SGATAYY15 pKa = 9.95RR16 pKa = 11.84ATVYY20 pKa = 10.79LGIDD24 pKa = 3.46VLTGKK29 pKa = 7.98TVTTSISARR38 pKa = 11.84TKK40 pKa = 10.45RR41 pKa = 11.84EE42 pKa = 3.44IKK44 pKa = 10.55AKK46 pKa = 10.22EE47 pKa = 3.73KK48 pKa = 11.07AKK50 pKa = 10.57LKK52 pKa = 9.94EE53 pKa = 4.1WLEE56 pKa = 4.26SGCTKK61 pKa = 10.31KK62 pKa = 10.69KK63 pKa = 10.07KK64 pKa = 10.36NSVKK68 pKa = 9.85TFNEE72 pKa = 4.31LVEE75 pKa = 4.19SWKK78 pKa = 10.86LSVYY82 pKa = 10.37PSYY85 pKa = 11.01KK86 pKa = 9.53YY87 pKa = 8.67HH88 pKa = 6.48TKK90 pKa = 9.12VTFDD94 pKa = 3.57NQLKK98 pKa = 10.37YY99 pKa = 10.97YY100 pKa = 9.85ILPAYY105 pKa = 10.25GDD107 pKa = 3.43IKK109 pKa = 10.94LEE111 pKa = 3.79KK112 pKa = 9.03LTRR115 pKa = 11.84FSIQEE120 pKa = 4.27TVNTWADD127 pKa = 3.13NYY129 pKa = 11.05NNNKK133 pKa = 8.83GKK135 pKa = 9.66PYY137 pKa = 10.69KK138 pKa = 10.73LYY140 pKa = 10.86DD141 pKa = 3.34SLFNLIHH148 pKa = 6.74TILGYY153 pKa = 10.04GVSVGFLEE161 pKa = 4.61NNPANDD167 pKa = 3.4IIVPKK172 pKa = 10.24RR173 pKa = 11.84KK174 pKa = 9.17KK175 pKa = 10.2DD176 pKa = 3.34KK177 pKa = 10.24PKK179 pKa = 10.45RR180 pKa = 11.84KK181 pKa = 9.19HH182 pKa = 5.9FEE184 pKa = 4.09PNEE187 pKa = 3.88VKK189 pKa = 10.17TFIVYY194 pKa = 10.47LDD196 pKa = 3.78QLDD199 pKa = 4.0DD200 pKa = 3.67TDD202 pKa = 6.25FRR204 pKa = 11.84TLTNKK209 pKa = 9.45VLFSLLLASGCRR221 pKa = 11.84IGEE224 pKa = 4.0ALALEE229 pKa = 4.4WSDD232 pKa = 4.12VDD234 pKa = 4.89FDD236 pKa = 4.42NKK238 pKa = 9.98TISISKK244 pKa = 8.19TLQHH248 pKa = 6.27NLKK251 pKa = 8.82TAPTKK256 pKa = 10.07TKK258 pKa = 10.2AGTRR262 pKa = 11.84IITLDD267 pKa = 3.65NDD269 pKa = 3.9TMRR272 pKa = 11.84LLNNYY277 pKa = 8.16QKK279 pKa = 10.45HH280 pKa = 4.26QRR282 pKa = 11.84LNGIMTGIVFSSRR295 pKa = 11.84RR296 pKa = 11.84GYY298 pKa = 8.37KK299 pKa = 8.3TSSSFGHH306 pKa = 5.62YY307 pKa = 9.18LKK309 pKa = 10.59KK310 pKa = 10.86YY311 pKa = 10.27LADD314 pKa = 4.23CGLPVLGFHH323 pKa = 7.82AFRR326 pKa = 11.84HH327 pKa = 4.68THH329 pKa = 6.22ASLMMNADD337 pKa = 3.88MQPKK341 pKa = 8.73EE342 pKa = 3.69LQYY345 pKa = 11.53RR346 pKa = 11.84LGHH349 pKa = 5.8SNIATTLDD357 pKa = 3.51IYY359 pKa = 11.48SHH361 pKa = 6.77LSKK364 pKa = 10.88EE365 pKa = 4.26KK366 pKa = 9.46EE367 pKa = 4.2KK368 pKa = 11.02EE369 pKa = 3.92SATIFQNALNSLL381 pKa = 3.95

Molecular weight: 43.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14

0

14

2546

56

455

181.9

20.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.009 ± 0.384

0.511 ± 0.085

6.991 ± 0.632

7.188 ± 0.569

3.456 ± 0.397

5.185 ± 0.472

1.728 ± 0.308

6.52 ± 0.347

9.191 ± 0.726

10.33 ± 0.82

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.317 ± 0.296

6.167 ± 0.241

2.828 ± 0.435

3.692 ± 0.382

5.185 ± 0.538

5.067 ± 0.6

7.148 ± 0.438

5.185 ± 0.332

0.864 ± 0.26

4.438 ± 0.326
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski