Cronobacter phage ESSI-2
Average proteome isoelectric point is 7.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F1BUL8|F1BUL8_9CAUD Phage head completion protein OS=Cronobacter phage ESSI-2 OX=947842 PE=4 SV=1
MM1 pKa = 7.07 KK2 pKa = 9.38 TQLEE6 pKa = 4.49 SLTEE10 pKa = 4.06 FFSEE14 pKa = 4.74 NIPEE18 pKa = 4.31 RR19 pKa = 11.84 ARR21 pKa = 11.84 QGFDD25 pKa = 3.17 SVIDD29 pKa = 3.82 EE30 pKa = 4.4 MQVVPAAKK38 pKa = 10.53 DD39 pKa = 3.22 MGLDD43 pKa = 3.31 QYY45 pKa = 11.03 RR46 pKa = 11.84 QALIRR51 pKa = 11.84 YY52 pKa = 7.29 NALLSWEE59 pKa = 4.09 RR60 pKa = 11.84 FPYY63 pKa = 10.09 RR64 pKa = 11.84 LCPPQLLVSLLEE76 pKa = 4.07 VWLDD80 pKa = 3.73 EE81 pKa = 4.41 YY82 pKa = 11.38 GSEE85 pKa = 4.23 LMEE88 pKa = 4.38 HH89 pKa = 6.31 VGITDD94 pKa = 5.54 ADD96 pKa = 4.08 PQWDD100 pKa = 3.99 VTVEE104 pKa = 3.99 DD105 pKa = 4.83 AEE107 pKa = 4.48 TATVVLSVPLVEE119 pKa = 4.28 EE120 pKa = 3.77 LVIRR124 pKa = 11.84 RR125 pKa = 11.84 DD126 pKa = 3.46 EE127 pKa = 4.25 KK128 pKa = 10.72 GAIPFRR134 pKa = 11.84 GEE136 pKa = 3.31 RR137 pKa = 11.84 WSLVDD142 pKa = 3.55 PEE144 pKa = 4.0 IWTALSAQVYY154 pKa = 9.89 GVDD157 pKa = 3.53 EE158 pKa = 4.51 TGAPIGDD165 pKa = 3.61 AA166 pKa = 4.0
Molecular weight: 18.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.332
IPC2_protein 4.164
IPC_protein 4.101
Toseland 3.923
ProMoST 4.215
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.935
Rodwell 3.923
Grimsley 3.834
Solomon 4.05
Lehninger 3.999
Nozaki 4.164
DTASelect 4.317
Thurlkill 3.935
EMBOSS 3.948
Sillero 4.202
Patrickios 3.719
IPC_peptide 4.05
IPC2_peptide 4.19
IPC2.peptide.svr19 4.131
Protein with the highest isoelectric point:
>tr|F1BUN8|F1BUN8_9CAUD CI repressor protein OS=Cronobacter phage ESSI-2 OX=947842 PE=4 SV=1
MM1 pKa = 7.38 CRR3 pKa = 11.84 MWLVNNRR10 pKa = 11.84 CHH12 pKa = 6.64 WMTKK16 pKa = 6.76 VTTNRR21 pKa = 11.84 RR22 pKa = 11.84 FCLMRR27 pKa = 11.84 VSAATDD33 pKa = 3.32 KK34 pKa = 11.27 AKK36 pKa = 10.72 RR37 pKa = 11.84 PVLGVSHH44 pKa = 6.95 TKK46 pKa = 10.99 DD47 pKa = 2.87 NDD49 pKa = 3.55 ARR51 pKa = 11.84 WIPLKK56 pKa = 9.39 TFCDD60 pKa = 3.25 RR61 pKa = 11.84 VGIRR65 pKa = 11.84 VRR67 pKa = 11.84 TGRR70 pKa = 11.84 YY71 pKa = 6.14 WYY73 pKa = 9.75 QIGRR77 pKa = 11.84 IRR79 pKa = 11.84 IRR81 pKa = 11.84 PKK83 pKa = 10.44 VKK85 pKa = 10.19 PKK87 pKa = 10.3 DD88 pKa = 3.82 HH89 pKa = 6.59 VWVDD93 pKa = 3.09 WYY95 pKa = 11.03 AWQEE99 pKa = 3.98 GKK101 pKa = 10.52
Molecular weight: 12.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.332
IPC2_protein 9.809
IPC_protein 10.701
Toseland 10.979
ProMoST 10.804
Dawson 11.038
Bjellqvist 10.774
Wikipedia 11.272
Rodwell 11.242
Grimsley 11.067
Solomon 11.213
Lehninger 11.184
Nozaki 10.965
DTASelect 10.774
Thurlkill 10.965
EMBOSS 11.389
Sillero 10.979
Patrickios 10.979
IPC_peptide 11.228
IPC2_peptide 9.984
IPC2.peptide.svr19 8.574
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
43
0
43
10733
99
691
249.6
27.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.404 ± 0.506
1.323 ± 0.215
4.966 ± 0.254
4.929 ± 0.347
3.95 ± 0.209
7.556 ± 0.4
2.245 ± 0.322
4.761 ± 0.257
4.789 ± 0.319
8.935 ± 0.333
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.46 ± 0.161
3.969 ± 0.207
4.342 ± 0.189
4.463 ± 0.273
7.314 ± 0.552
5.674 ± 0.462
5.562 ± 0.331
6.904 ± 0.279
1.789 ± 0.165
2.665 ± 0.242
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here