Cronobacter phage ESSI-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Seongnamvirus; Cronobacter virus ESSI2

Average proteome isoelectric point is 7.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F1BUL8|F1BUL8_9CAUD Phage head completion protein OS=Cronobacter phage ESSI-2 OX=947842 PE=4 SV=1
MM1 pKa = 7.07KK2 pKa = 9.38TQLEE6 pKa = 4.49SLTEE10 pKa = 4.06FFSEE14 pKa = 4.74NIPEE18 pKa = 4.31RR19 pKa = 11.84ARR21 pKa = 11.84QGFDD25 pKa = 3.17SVIDD29 pKa = 3.82EE30 pKa = 4.4MQVVPAAKK38 pKa = 10.53DD39 pKa = 3.22MGLDD43 pKa = 3.31QYY45 pKa = 11.03RR46 pKa = 11.84QALIRR51 pKa = 11.84YY52 pKa = 7.29NALLSWEE59 pKa = 4.09RR60 pKa = 11.84FPYY63 pKa = 10.09RR64 pKa = 11.84LCPPQLLVSLLEE76 pKa = 4.07VWLDD80 pKa = 3.73EE81 pKa = 4.41YY82 pKa = 11.38GSEE85 pKa = 4.23LMEE88 pKa = 4.38HH89 pKa = 6.31VGITDD94 pKa = 5.54ADD96 pKa = 4.08PQWDD100 pKa = 3.99VTVEE104 pKa = 3.99DD105 pKa = 4.83AEE107 pKa = 4.48TATVVLSVPLVEE119 pKa = 4.28EE120 pKa = 3.77LVIRR124 pKa = 11.84RR125 pKa = 11.84DD126 pKa = 3.46EE127 pKa = 4.25KK128 pKa = 10.72GAIPFRR134 pKa = 11.84GEE136 pKa = 3.31RR137 pKa = 11.84WSLVDD142 pKa = 3.55PEE144 pKa = 4.0IWTALSAQVYY154 pKa = 9.89GVDD157 pKa = 3.53EE158 pKa = 4.51TGAPIGDD165 pKa = 3.61AA166 pKa = 4.0

Molecular weight: 18.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F1BUN8|F1BUN8_9CAUD CI repressor protein OS=Cronobacter phage ESSI-2 OX=947842 PE=4 SV=1
MM1 pKa = 7.38CRR3 pKa = 11.84MWLVNNRR10 pKa = 11.84CHH12 pKa = 6.64WMTKK16 pKa = 6.76VTTNRR21 pKa = 11.84RR22 pKa = 11.84FCLMRR27 pKa = 11.84VSAATDD33 pKa = 3.32KK34 pKa = 11.27AKK36 pKa = 10.72RR37 pKa = 11.84PVLGVSHH44 pKa = 6.95TKK46 pKa = 10.99DD47 pKa = 2.87NDD49 pKa = 3.55ARR51 pKa = 11.84WIPLKK56 pKa = 9.39TFCDD60 pKa = 3.25RR61 pKa = 11.84VGIRR65 pKa = 11.84VRR67 pKa = 11.84TGRR70 pKa = 11.84YY71 pKa = 6.14WYY73 pKa = 9.75QIGRR77 pKa = 11.84IRR79 pKa = 11.84IRR81 pKa = 11.84PKK83 pKa = 10.44VKK85 pKa = 10.19PKK87 pKa = 10.3DD88 pKa = 3.82HH89 pKa = 6.59VWVDD93 pKa = 3.09WYY95 pKa = 11.03AWQEE99 pKa = 3.98GKK101 pKa = 10.52

Molecular weight: 12.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

10733

99

691

249.6

27.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.404 ± 0.506

1.323 ± 0.215

4.966 ± 0.254

4.929 ± 0.347

3.95 ± 0.209

7.556 ± 0.4

2.245 ± 0.322

4.761 ± 0.257

4.789 ± 0.319

8.935 ± 0.333

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.46 ± 0.161

3.969 ± 0.207

4.342 ± 0.189

4.463 ± 0.273

7.314 ± 0.552

5.674 ± 0.462

5.562 ± 0.331

6.904 ± 0.279

1.789 ± 0.165

2.665 ± 0.242
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski