Erythrobacter longus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria;

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3219 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A074M7W5|A0A074M7W5_ERYLO Disulfide bond formation protein OS=Erythrobacter longus OX=1044 GN=EH31_12730 PE=4 SV=1
MM1 pKa = 7.25TKK3 pKa = 9.85IFRR6 pKa = 11.84TAAAGVALFAAVGLGSAAHH25 pKa = 5.76AQVTEE30 pKa = 4.32SADD33 pKa = 3.18ATAEE37 pKa = 4.04VLAALQLTNDD47 pKa = 3.5AGLDD51 pKa = 3.61FGAIVVNAGSTGGSVAVDD69 pKa = 3.5FTGFRR74 pKa = 11.84NCAGDD79 pKa = 4.01VICGPGGTEE88 pKa = 3.38QAAAFTVTGAAGTNVSVSVQDD109 pKa = 4.37LGANPVVLTHH119 pKa = 6.55TGFAGSTSNEE129 pKa = 3.78HH130 pKa = 6.8LIEE133 pKa = 4.24LTSLQDD139 pKa = 3.28GSFGGFPFFNGSEE152 pKa = 4.17TFNVGGTISLDD163 pKa = 3.25GTEE166 pKa = 3.82IAGTYY171 pKa = 9.25EE172 pKa = 3.84GSFDD176 pKa = 4.14VTVQYY181 pKa = 10.92QQ182 pKa = 2.97

Molecular weight:
18.05 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A074M8S7|A0A074M8S7_ERYLO Short-chain dehydrogenase OS=Erythrobacter longus OX=1044 GN=EH31_00520 PE=3 SV=1
MM1 pKa = 7.05NAKK4 pKa = 10.05AGNEE8 pKa = 4.09PKK10 pKa = 10.68GSNAEE15 pKa = 3.86RR16 pKa = 11.84LALRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84MEE25 pKa = 4.0SGPAIFRR32 pKa = 11.84GAFRR36 pKa = 11.84PFFLGAAFWAIFTMCLWLGHH56 pKa = 5.9VLGQIPGGFVSDD68 pKa = 3.4ALAWHH73 pKa = 5.87RR74 pKa = 11.84HH75 pKa = 3.89EE76 pKa = 4.45MLFGFVGAAIAGFALTSAPNWTGRR100 pKa = 11.84LPIAGWPLATLFFFWCVGRR119 pKa = 11.84VLPFGLADD127 pKa = 3.92TSAALIALDD136 pKa = 3.36GGFYY140 pKa = 10.82LFLSFLIGRR149 pKa = 11.84EE150 pKa = 3.91EE151 pKa = 3.75THH153 pKa = 5.44GRR155 pKa = 11.84KK156 pKa = 9.22RR157 pKa = 11.84SLTICMVIAAFGFADD172 pKa = 3.03ILDD175 pKa = 4.0RR176 pKa = 11.84MEE178 pKa = 3.69MAKK181 pKa = 10.09WIDD184 pKa = 3.52SAGLGWRR191 pKa = 11.84AGIALVTALVMLIGGRR207 pKa = 11.84LIPSFTHH214 pKa = 5.64NWLLKK219 pKa = 8.67QVRR222 pKa = 11.84LDD224 pKa = 3.73PTPTQPDD231 pKa = 3.45LTDD234 pKa = 5.13AIVMAVTIGGLLVWILARR252 pKa = 11.84ASGASGTLLMLAGLANFARR271 pKa = 11.84LARR274 pKa = 11.84WQGWRR279 pKa = 11.84CLPCLPVFVLHH290 pKa = 7.37LGYY293 pKa = 10.41FWLALGLLLLGAAQFGWISEE313 pKa = 4.35SPAIHH318 pKa = 6.52ALSTGAMATMILGVMSRR335 pKa = 11.84TSLAMTARR343 pKa = 11.84EE344 pKa = 4.01PSFNLAIASACVLITLAAIARR365 pKa = 11.84VVAGLQIGQSFVLLVVAGFGWLGAFGLFLVVFGPILSSPRR405 pKa = 11.84KK406 pKa = 9.08GALGKK411 pKa = 10.34

Molecular weight:
44.09 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3219

0

3219

1072962

41

7517

333.3

36.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.987 ± 0.059

0.807 ± 0.017

6.194 ± 0.064

6.495 ± 0.042

3.903 ± 0.034

8.816 ± 0.056

1.809 ± 0.027

5.287 ± 0.027

3.477 ± 0.049

9.539 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.444 ± 0.028

3.013 ± 0.035

4.859 ± 0.038

3.355 ± 0.025

6.33 ± 0.05

5.89 ± 0.037

5.37 ± 0.042

6.901 ± 0.033

1.351 ± 0.02

2.176 ± 0.023

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski