Erythrobacter longus
Average proteome isoelectric point is 5.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3219 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A074M7W5|A0A074M7W5_ERYLO Disulfide bond formation protein OS=Erythrobacter longus OX=1044 GN=EH31_12730 PE=4 SV=1
MM1 pKa = 7.25 TKK3 pKa = 9.85 IFRR6 pKa = 11.84 TAAAGVALFAAVGLGSAAHH25 pKa = 5.76 AQVTEE30 pKa = 4.32 SADD33 pKa = 3.18 ATAEE37 pKa = 4.04 VLAALQLTNDD47 pKa = 3.5 AGLDD51 pKa = 3.61 FGAIVVNAGSTGGSVAVDD69 pKa = 3.5 FTGFRR74 pKa = 11.84 NCAGDD79 pKa = 4.01 VICGPGGTEE88 pKa = 3.38 QAAAFTVTGAAGTNVSVSVQDD109 pKa = 4.37 LGANPVVLTHH119 pKa = 6.55 TGFAGSTSNEE129 pKa = 3.78 HH130 pKa = 6.8 LIEE133 pKa = 4.24 LTSLQDD139 pKa = 3.28 GSFGGFPFFNGSEE152 pKa = 4.17 TFNVGGTISLDD163 pKa = 3.25 GTEE166 pKa = 3.82 IAGTYY171 pKa = 9.25 EE172 pKa = 3.84 GSFDD176 pKa = 4.14 VTVQYY181 pKa = 10.92 QQ182 pKa = 2.97
Molecular weight: 18.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.762
IPC2_protein 3.91
IPC_protein 3.846
Toseland 3.656
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.757
Rodwell 3.681
Grimsley 3.567
Solomon 3.808
Lehninger 3.77
Nozaki 3.961
DTASelect 4.139
Thurlkill 3.706
EMBOSS 3.77
Sillero 3.961
Patrickios 1.888
IPC_peptide 3.808
IPC2_peptide 3.948
IPC2.peptide.svr19 3.868
Protein with the highest isoelectric point:
>tr|A0A074M8S7|A0A074M8S7_ERYLO Short-chain dehydrogenase OS=Erythrobacter longus OX=1044 GN=EH31_00520 PE=3 SV=1
MM1 pKa = 7.05 NAKK4 pKa = 10.05 AGNEE8 pKa = 4.09 PKK10 pKa = 10.68 GSNAEE15 pKa = 3.86 RR16 pKa = 11.84 LALRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 RR23 pKa = 11.84 MEE25 pKa = 4.0 SGPAIFRR32 pKa = 11.84 GAFRR36 pKa = 11.84 PFFLGAAFWAIFTMCLWLGHH56 pKa = 5.9 VLGQIPGGFVSDD68 pKa = 3.4 ALAWHH73 pKa = 5.87 RR74 pKa = 11.84 HH75 pKa = 3.89 EE76 pKa = 4.45 MLFGFVGAAIAGFALTSAPNWTGRR100 pKa = 11.84 LPIAGWPLATLFFFWCVGRR119 pKa = 11.84 VLPFGLADD127 pKa = 3.92 TSAALIALDD136 pKa = 3.36 GGFYY140 pKa = 10.82 LFLSFLIGRR149 pKa = 11.84 EE150 pKa = 3.91 EE151 pKa = 3.75 THH153 pKa = 5.44 GRR155 pKa = 11.84 KK156 pKa = 9.22 RR157 pKa = 11.84 SLTICMVIAAFGFADD172 pKa = 3.03 ILDD175 pKa = 4.0 RR176 pKa = 11.84 MEE178 pKa = 3.69 MAKK181 pKa = 10.09 WIDD184 pKa = 3.52 SAGLGWRR191 pKa = 11.84 AGIALVTALVMLIGGRR207 pKa = 11.84 LIPSFTHH214 pKa = 5.64 NWLLKK219 pKa = 8.67 QVRR222 pKa = 11.84 LDD224 pKa = 3.73 PTPTQPDD231 pKa = 3.45 LTDD234 pKa = 5.13 AIVMAVTIGGLLVWILARR252 pKa = 11.84 ASGASGTLLMLAGLANFARR271 pKa = 11.84 LARR274 pKa = 11.84 WQGWRR279 pKa = 11.84 CLPCLPVFVLHH290 pKa = 7.37 LGYY293 pKa = 10.41 FWLALGLLLLGAAQFGWISEE313 pKa = 4.35 SPAIHH318 pKa = 6.52 ALSTGAMATMILGVMSRR335 pKa = 11.84 TSLAMTARR343 pKa = 11.84 EE344 pKa = 4.01 PSFNLAIASACVLITLAAIARR365 pKa = 11.84 VVAGLQIGQSFVLLVVAGFGWLGAFGLFLVVFGPILSSPRR405 pKa = 11.84 KK406 pKa = 9.08 GALGKK411 pKa = 10.34
Molecular weight: 44.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.385
IPC_protein 10.072
Toseland 10.628
ProMoST 10.394
Dawson 10.687
Bjellqvist 10.423
Wikipedia 10.906
Rodwell 10.804
Grimsley 10.73
Solomon 10.847
Lehninger 10.818
Nozaki 10.657
DTASelect 10.409
Thurlkill 10.613
EMBOSS 11.038
Sillero 10.643
Patrickios 10.54
IPC_peptide 10.862
IPC2_peptide 9.78
IPC2.peptide.svr19 8.635
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3219
0
3219
1072962
41
7517
333.3
36.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.987 ± 0.059
0.807 ± 0.017
6.194 ± 0.064
6.495 ± 0.042
3.903 ± 0.034
8.816 ± 0.056
1.809 ± 0.027
5.287 ± 0.027
3.477 ± 0.049
9.539 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.444 ± 0.028
3.013 ± 0.035
4.859 ± 0.038
3.355 ± 0.025
6.33 ± 0.05
5.89 ± 0.037
5.37 ± 0.042
6.901 ± 0.033
1.351 ± 0.02
2.176 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here