Zymoseptoria brevis

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Zymoseptoria

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10566 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F4GJB6|A0A0F4GJB6_9PEZI Eukaryotic translation initiation factor 3 subunit J OS=Zymoseptoria brevis OX=1047168 GN=HCR1 PE=3 SV=1
MM1 pKa = 7.41GGAISFEE8 pKa = 4.61SNQLAGAINGFRR20 pKa = 11.84STQVFSTSYY29 pKa = 10.98GFDD32 pKa = 3.29PSGSNLFGIIATVSAEE48 pKa = 4.17STSLGVTPIIVFPVTTVEE66 pKa = 4.92AGDD69 pKa = 3.83ASKK72 pKa = 9.51STVTCTPFNDD82 pKa = 4.2GALPTAAAATNQLTCQIANTAIAPGVASVRR112 pKa = 11.84FRR114 pKa = 11.84DD115 pKa = 4.53ANDD118 pKa = 3.23EE119 pKa = 4.44PYY121 pKa = 11.27DD122 pKa = 3.74DD123 pKa = 5.7FYY125 pKa = 11.72VFSIAAPTSLSTSTTIATSITVVDD149 pKa = 4.96GSTVGSTTVTEE160 pKa = 4.52TTFSSTVTEE169 pKa = 4.72TPTSTLTGASSTTYY183 pKa = 10.07VSCSTTTSGTATGTATSSSGSSSTSSGSATTGIDD217 pKa = 3.28SSSGGSSSTSSGSATTGIDD236 pKa = 3.61YY237 pKa = 10.99SSTASNTGIADD248 pKa = 4.08PGTTATASSSTASDD262 pKa = 3.47TATGTTGNDD271 pKa = 3.23SSSNTALAPLYY282 pKa = 7.94PTDD285 pKa = 4.46SNTSSTSCTTAPSSNTTLKK304 pKa = 10.73KK305 pKa = 10.09VIKK308 pKa = 9.14TIIVTDD314 pKa = 4.14CGRR317 pKa = 11.84AAAQTPALHH326 pKa = 6.52PRR328 pKa = 11.84QTTEE332 pKa = 3.67TTILASGFSGPDD344 pKa = 3.23FTFSEE349 pKa = 4.76GNNVGTVTSTSTSVEE364 pKa = 4.19TQSTVVTSGTDD375 pKa = 3.38TVVEE379 pKa = 4.64TSAVTTSTVSTTTVVLDD396 pKa = 3.7EE397 pKa = 4.34RR398 pKa = 11.84TVATVSGCEE407 pKa = 3.65PAAPTFTGG415 pKa = 3.37

Molecular weight: 40.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F4GU16|A0A0F4GU16_9PEZI ATP synthase subunit e mitochondrial OS=Zymoseptoria brevis OX=1047168 GN=TI39_contig307g00085 PE=3 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.91KK5 pKa = 10.39SFRR8 pKa = 11.84TKK10 pKa = 10.45QKK12 pKa = 9.84LAKK15 pKa = 9.55AQKK18 pKa = 8.59QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.15WRR45 pKa = 11.84KK46 pKa = 7.38TRR48 pKa = 11.84IGII51 pKa = 4.0

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10566

0

10566

5141735

45

7061

486.6

53.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.157 ± 0.023

1.156 ± 0.008

5.853 ± 0.017

6.349 ± 0.023

3.526 ± 0.013

7.032 ± 0.022

2.401 ± 0.011

4.52 ± 0.016

4.904 ± 0.023

8.542 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.246 ± 0.009

3.599 ± 0.013

6.034 ± 0.024

4.124 ± 0.019

6.192 ± 0.022

8.153 ± 0.025

6.164 ± 0.021

6.038 ± 0.015

1.392 ± 0.009

2.619 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski