Lactobacillus versmoldensis DSM 14857 = KCTC 3814
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2261 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R1SDH1|A0A0R1SDH1_9LACO Na(+) H(+) antiporter OS=Lactobacillus versmoldensis DSM 14857 = KCTC 3814 OX=1423815 GN=FC27_GL002048 PE=4 SV=1
MM1 pKa = 7.17 TKK3 pKa = 10.51 VKK5 pKa = 9.98 IVYY8 pKa = 10.36 ASITGNDD15 pKa = 2.9 EE16 pKa = 4.44 DD17 pKa = 3.41 IAYY20 pKa = 10.67 VLTEE24 pKa = 4.18 KK25 pKa = 10.94 FEE27 pKa = 4.17 EE28 pKa = 4.32 LNADD32 pKa = 3.5 VEE34 pKa = 4.45 MSEE37 pKa = 5.04 VSQTDD42 pKa = 3.24 PSDD45 pKa = 4.53 FEE47 pKa = 5.2 DD48 pKa = 5.09 ADD50 pKa = 3.35 ICIIASYY57 pKa = 9.46 TYY59 pKa = 10.42 DD60 pKa = 3.14 QGIVPEE66 pKa = 4.23 EE67 pKa = 3.88 ALDD70 pKa = 4.05 FYY72 pKa = 11.51 EE73 pKa = 6.12 DD74 pKa = 4.48 MEE76 pKa = 5.63 DD77 pKa = 4.31 LDD79 pKa = 4.4 LTGKK83 pKa = 10.69 VYY85 pKa = 8.15 GTCGSGDD92 pKa = 3.3 TFYY95 pKa = 11.44 EE96 pKa = 4.48 DD97 pKa = 3.12 FCSAVDD103 pKa = 3.74 EE104 pKa = 4.51 FAKK107 pKa = 10.44 TFDD110 pKa = 3.45 EE111 pKa = 4.56 TGATKK116 pKa = 10.36 GAEE119 pKa = 4.11 SVKK122 pKa = 10.36 IEE124 pKa = 4.71 LEE126 pKa = 4.12 PEE128 pKa = 3.54 QDD130 pKa = 5.71 DD131 pKa = 4.7 IDD133 pKa = 4.29 NLDD136 pKa = 3.63 KK137 pKa = 10.77 FAEE140 pKa = 4.11 QLYY143 pKa = 8.33 KK144 pKa = 9.49 TAKK147 pKa = 10.35 EE148 pKa = 3.94 MGLL151 pKa = 3.59
Molecular weight: 16.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.772
IPC2_protein 3.643
IPC_protein 3.63
Toseland 3.427
ProMoST 3.783
Dawson 3.617
Bjellqvist 3.77
Wikipedia 3.541
Rodwell 3.465
Grimsley 3.338
Solomon 3.605
Lehninger 3.554
Nozaki 3.732
DTASelect 3.935
Thurlkill 3.478
EMBOSS 3.554
Sillero 3.745
Patrickios 1.812
IPC_peptide 3.605
IPC2_peptide 3.732
IPC2.peptide.svr19 3.714
Protein with the highest isoelectric point:
>tr|A0A0R1SGM0|A0A0R1SGM0_9LACO Uncharacterized protein OS=Lactobacillus versmoldensis DSM 14857 = KCTC 3814 OX=1423815 GN=FC27_GL001598 PE=4 SV=1
MM1 pKa = 7.57 KK2 pKa = 9.98 PLIQEE7 pKa = 3.7 ITKK10 pKa = 9.54 EE11 pKa = 4.01 QLRR14 pKa = 11.84 SDD16 pKa = 3.33 IPEE19 pKa = 3.83 FRR21 pKa = 11.84 AGDD24 pKa = 4.19 TIRR27 pKa = 11.84 VHH29 pKa = 6.42 AKK31 pKa = 8.82 VVEE34 pKa = 4.41 GTRR37 pKa = 11.84 EE38 pKa = 3.89 RR39 pKa = 11.84 IQLFEE44 pKa = 3.91 GVVIKK49 pKa = 10.66 RR50 pKa = 11.84 HH51 pKa = 4.97 GAGISATYY59 pKa = 8.17 TVRR62 pKa = 11.84 KK63 pKa = 8.0 MSSGVGVEE71 pKa = 3.93 RR72 pKa = 11.84 TFPLNTPRR80 pKa = 11.84 VDD82 pKa = 3.3 KK83 pKa = 11.12 IEE85 pKa = 4.02 VVRR88 pKa = 11.84 YY89 pKa = 8.01 GQVRR93 pKa = 11.84 RR94 pKa = 11.84 AKK96 pKa = 10.32 LYY98 pKa = 8.74 YY99 pKa = 10.08 LRR101 pKa = 11.84 NRR103 pKa = 11.84 TGKK106 pKa = 9.82 AARR109 pKa = 11.84 IKK111 pKa = 10.22 EE112 pKa = 3.99 RR113 pKa = 11.84 RR114 pKa = 11.84 RR115 pKa = 11.84 DD116 pKa = 3.21 II117 pKa = 4.17
Molecular weight: 13.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 9.882
IPC_protein 10.921
Toseland 11.067
ProMoST 11.082
Dawson 11.125
Bjellqvist 10.921
Wikipedia 11.418
Rodwell 11.213
Grimsley 11.155
Solomon 11.374
Lehninger 11.316
Nozaki 11.052
DTASelect 10.921
Thurlkill 11.052
EMBOSS 11.506
Sillero 11.067
Patrickios 10.95
IPC_peptide 11.389
IPC2_peptide 10.028
IPC2.peptide.svr19 8.65
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2261
0
2261
688665
50
2960
304.6
34.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.995 ± 0.052
0.467 ± 0.013
6.476 ± 0.054
5.455 ± 0.055
4.397 ± 0.04
6.539 ± 0.046
1.911 ± 0.024
7.775 ± 0.051
7.255 ± 0.06
9.379 ± 0.115
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.666 ± 0.027
5.528 ± 0.05
3.516 ± 0.038
4.259 ± 0.045
3.414 ± 0.038
6.563 ± 0.05
5.985 ± 0.047
6.915 ± 0.041
0.941 ± 0.019
3.564 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here