Paxillus involutus ATCC 200175
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11689 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C9TB44|A0A0C9TB44_PAXIN Unplaced genomic scaffold PAXINscaffold_37 whole genome shotgun sequence OS=Paxillus involutus ATCC 200175 OX=664439 GN=PAXINDRAFT_156773 PE=4 SV=1
MM1 pKa = 7.43 INVFTSLLDD10 pKa = 3.53 VGPTAEE16 pKa = 4.51 GEE18 pKa = 4.44 IIAPMADD25 pKa = 3.08 NLLAAGKK32 pKa = 7.26 WLKK35 pKa = 10.55 YY36 pKa = 10.4 AGEE39 pKa = 4.34 CVYY42 pKa = 10.45 ATDD45 pKa = 3.29 YY46 pKa = 10.46 WYY48 pKa = 8.27 QTSQDD53 pKa = 3.52 PTGSFRR59 pKa = 11.84 FLTTPQTFCIVAFNKK74 pKa = 7.34 PTNGSVVVNAGGVVLPIQQGDD95 pKa = 4.38 AIRR98 pKa = 11.84 LLGPNSPGVFSDD110 pKa = 3.58 DD111 pKa = 3.05 TTAQTSGLEE120 pKa = 3.74 WRR122 pKa = 11.84 MDD124 pKa = 3.51 EE125 pKa = 5.65 DD126 pKa = 4.11 GVLTIDD132 pKa = 3.83 VPEE135 pKa = 4.58 DD136 pKa = 3.19 QVDD139 pKa = 3.72 QVDD142 pKa = 4.18 YY143 pKa = 10.82 AWAFQVSYY151 pKa = 11.67 ALFF154 pKa = 4.12
Molecular weight: 16.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.719
IPC_protein 3.694
Toseland 3.478
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.668
Rodwell 3.516
Grimsley 3.389
Solomon 3.681
Lehninger 3.643
Nozaki 3.821
DTASelect 4.075
Thurlkill 3.541
EMBOSS 3.668
Sillero 3.821
Patrickios 0.846
IPC_peptide 3.668
IPC2_peptide 3.783
IPC2.peptide.svr19 3.74
Protein with the highest isoelectric point:
>tr|A0A0C9SN70|A0A0C9SN70_PAXIN Unplaced genomic scaffold PAXINscaffold_580 whole genome shotgun sequence (Fragment) OS=Paxillus involutus ATCC 200175 OX=664439 GN=PAXINDRAFT_90432 PE=4 SV=1
MM1 pKa = 7.27 AQHH4 pKa = 6.07 FHH6 pKa = 6.16 FALRR10 pKa = 11.84 ALAYY14 pKa = 9.94 VAISLCPCFRR24 pKa = 11.84 QVIVTSPGVNGAVRR38 pKa = 11.84 LLVNILGWNEE48 pKa = 4.03 SLSLLSSRR56 pKa = 11.84 FCNILEE62 pKa = 4.31 AFHH65 pKa = 6.68 SRR67 pKa = 11.84 LHH69 pKa = 5.35 IVARR73 pKa = 11.84 QTQQNGVASVDD84 pKa = 3.38 ASVDD88 pKa = 3.72 SLHH91 pKa = 6.97 LISLRR96 pKa = 11.84 LILPRR101 pKa = 11.84 YY102 pKa = 8.27 LARR105 pKa = 11.84 SPTQAHH111 pKa = 6.21 RR112 pKa = 11.84 AKK114 pKa = 10.72 FSIPPFILVFGKK126 pKa = 8.83 ATSTGVHH133 pKa = 5.97 SPSCASYY140 pKa = 10.39 HH141 pKa = 6.0 RR142 pKa = 11.84 HH143 pKa = 5.69 TGPLKK148 pKa = 10.73 DD149 pKa = 3.67 VTLSASVLNTATYY162 pKa = 10.81 CLARR166 pKa = 11.84 PPKK169 pKa = 10.09 QSPDD173 pKa = 3.01 PSFGVFYY180 pKa = 10.6 WEE182 pKa = 4.03 TSLVHH187 pKa = 6.29 LAGILIPGCRR197 pKa = 11.84 SSSRR201 pKa = 11.84 PPQHH205 pKa = 5.41 WKK207 pKa = 9.03 MLIVSQDD214 pKa = 3.24 RR215 pKa = 11.84 LIGGLTQTVSEE226 pKa = 4.71 GARR229 pKa = 11.84 TRR231 pKa = 11.84 SPARR235 pKa = 11.84 FMVDD239 pKa = 2.99 AARR242 pKa = 11.84 TEE244 pKa = 4.29 TGATLPQYY252 pKa = 11.13 KK253 pKa = 8.75 STTEE257 pKa = 3.73 AMSTSHH263 pKa = 6.56 SPHH266 pKa = 7.5 SMQTWHH272 pKa = 6.15 SRR274 pKa = 11.84 KK275 pKa = 10.2 GSTSFPAISVPPTTVVAIMVHH296 pKa = 6.5 PEE298 pKa = 4.0 SLHH301 pKa = 6.53 CLAQSRR307 pKa = 11.84 FGYY310 pKa = 10.11 LPPIRR315 pKa = 11.84 IASRR319 pKa = 11.84 RR320 pKa = 11.84 ACPEE324 pKa = 3.74 EE325 pKa = 4.03 DD326 pKa = 3.5 TGDD329 pKa = 3.71 LTAVWAQEE337 pKa = 3.71 WPAPSIGGRR346 pKa = 11.84 PCRR349 pKa = 11.84 NNGRR353 pKa = 11.84 RR354 pKa = 11.84 KK355 pKa = 9.37 FLVVSRR361 pKa = 11.84 RR362 pKa = 11.84 MNLPLKK368 pKa = 10.11 QAA370 pKa = 3.81
Molecular weight: 40.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.545
IPC_protein 10.335
Toseland 10.409
ProMoST 10.16
Dawson 10.555
Bjellqvist 10.321
Wikipedia 10.774
Rodwell 10.701
Grimsley 10.628
Solomon 10.643
Lehninger 10.613
Nozaki 10.467
DTASelect 10.292
Thurlkill 10.438
EMBOSS 10.818
Sillero 10.496
Patrickios 10.35
IPC_peptide 10.643
IPC2_peptide 9.663
IPC2.peptide.svr19 8.478
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11649
40
11689
3729177
49
3922
319.0
35.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.115 ± 0.023
1.523 ± 0.012
5.634 ± 0.019
5.898 ± 0.023
3.657 ± 0.016
6.471 ± 0.02
2.965 ± 0.013
4.856 ± 0.016
4.434 ± 0.019
9.165 ± 0.027
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.14 ± 0.01
3.46 ± 0.013
6.439 ± 0.031
3.862 ± 0.014
6.13 ± 0.024
8.422 ± 0.03
6.18 ± 0.018
6.471 ± 0.016
1.604 ± 0.012
2.576 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here