Cimodo virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; unclassified Reoviridae

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W5RUY0|W5RUY0_9REOV Uncharacterized protein OS=Cimodo virus OX=1427476 PE=4 SV=1
MM1 pKa = 7.6AAQPNNYY8 pKa = 9.39DD9 pKa = 3.29PQFFRR14 pKa = 11.84QATLLVDD21 pKa = 3.98TIRR24 pKa = 11.84RR25 pKa = 11.84YY26 pKa = 9.06ATDD29 pKa = 4.07KK30 pKa = 11.27FNFDD34 pKa = 3.34NARR37 pKa = 11.84NPVYY41 pKa = 9.81PANYY45 pKa = 9.49NLRR48 pKa = 11.84LGYY51 pKa = 9.48MPARR55 pKa = 11.84TSFPVFFDD63 pKa = 3.89TYY65 pKa = 10.32QPPIQSPRR73 pKa = 11.84EE74 pKa = 3.91YY75 pKa = 10.58LDD77 pKa = 3.05TFGPVQYY84 pKa = 9.52TVNDD88 pKa = 3.92ALTDD92 pKa = 3.64DD93 pKa = 4.55EE94 pKa = 4.71KK95 pKa = 11.39RR96 pKa = 11.84QVFQNIGDD104 pKa = 4.54LLQQIANAYY113 pKa = 8.71HH114 pKa = 6.04LQLNVEE120 pKa = 4.4QIDD123 pKa = 3.77VTEE126 pKa = 4.24TVPQLVYY133 pKa = 9.88TAFLLPDD140 pKa = 3.44GTVDD144 pKa = 3.31QDD146 pKa = 3.51YY147 pKa = 11.14ARR149 pKa = 11.84YY150 pKa = 7.77MQSTNPQNQDD160 pKa = 2.52VAQFEE165 pKa = 5.04LIRR168 pKa = 11.84ANGYY172 pKa = 10.35GGFEE176 pKa = 4.1FRR178 pKa = 11.84DD179 pKa = 3.53PAEE182 pKa = 4.55DD183 pKa = 3.42VLHH186 pKa = 7.04RR187 pKa = 11.84EE188 pKa = 4.45DD189 pKa = 4.34GNVIEE194 pKa = 4.31PAPRR198 pKa = 11.84RR199 pKa = 11.84RR200 pKa = 11.84IIDD203 pKa = 3.75PASDD207 pKa = 3.88DD208 pKa = 3.91SDD210 pKa = 3.9VDD212 pKa = 3.6EE213 pKa = 6.33DD214 pKa = 5.04DD215 pKa = 4.46VPEE218 pKa = 3.97QEE220 pKa = 4.1RR221 pKa = 11.84RR222 pKa = 11.84FAAEE226 pKa = 3.66QNRR229 pKa = 11.84QQSVTILKK237 pKa = 10.32GRR239 pKa = 11.84VIPIDD244 pKa = 3.61ALRR247 pKa = 11.84VRR249 pKa = 11.84CWYY252 pKa = 9.07EE253 pKa = 4.12GPAQIPDD260 pKa = 4.74AIDD263 pKa = 3.77FPAISDD269 pKa = 4.07EE270 pKa = 5.48IRR272 pKa = 11.84IQDD275 pKa = 3.49NSSSFDD281 pKa = 3.37FFACVSNAMMNGAFQDD297 pKa = 3.65YY298 pKa = 10.02NYY300 pKa = 11.22KK301 pKa = 10.44SFCTMLVNYY310 pKa = 9.24CWDD313 pKa = 3.46YY314 pKa = 11.46AISDD318 pKa = 4.06RR319 pKa = 11.84IGYY322 pKa = 9.23FVMRR326 pKa = 11.84MTKK329 pKa = 9.96YY330 pKa = 10.37PVGQVQAPRR339 pKa = 11.84EE340 pKa = 4.15VVATRR345 pKa = 11.84EE346 pKa = 3.86QLEE349 pKa = 4.18AKK351 pKa = 10.2PNGEE355 pKa = 5.28FYY357 pKa = 9.99WQMYY361 pKa = 9.42RR362 pKa = 11.84YY363 pKa = 6.87WTITKK368 pKa = 10.08RR369 pKa = 11.84LNDD372 pKa = 3.39EE373 pKa = 4.23ALASFVQPSINILANEE389 pKa = 3.98IGLFEE394 pKa = 4.74GGPGNVLNATQNIKK408 pKa = 10.41FQMEE412 pKa = 4.18DD413 pKa = 3.07RR414 pKa = 11.84QINYY418 pKa = 8.74PDD420 pKa = 4.47LRR422 pKa = 11.84LACPSLDD429 pKa = 3.67YY430 pKa = 10.78EE431 pKa = 4.36QLVLFPADD439 pKa = 2.99SFAVMRR445 pKa = 11.84VLVSAIMVTTANKK458 pKa = 9.94RR459 pKa = 11.84DD460 pKa = 3.76VLIDD464 pKa = 3.5PVNQARR470 pKa = 11.84YY471 pKa = 10.1NEE473 pKa = 3.87WTLYY477 pKa = 10.94DD478 pKa = 3.71MKK480 pKa = 10.98EE481 pKa = 4.09VTTKK485 pKa = 10.8SSLRR489 pKa = 11.84MKK491 pKa = 9.63WRR493 pKa = 11.84TSALICCMLLYY504 pKa = 10.66LLPDD508 pKa = 4.03DD509 pKa = 4.63

Molecular weight: 58.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W5RUY0|W5RUY0_9REOV Uncharacterized protein OS=Cimodo virus OX=1427476 PE=4 SV=1
MM1 pKa = 7.19ATIEE5 pKa = 4.46IPFSQATQYY14 pKa = 10.91EE15 pKa = 4.61HH16 pKa = 7.04IANKK20 pKa = 10.13RR21 pKa = 11.84NHH23 pKa = 5.62QLVNIIMEE31 pKa = 4.69ANQFLLRR38 pKa = 11.84HH39 pKa = 5.12QFVLSVNYY47 pKa = 9.54FGPKK51 pKa = 9.37EE52 pKa = 4.0YY53 pKa = 10.58EE54 pKa = 4.03PNAILHH60 pKa = 5.63PCYY63 pKa = 10.29FRR65 pKa = 11.84FALYY69 pKa = 10.57NNRR72 pKa = 11.84ASIPCRR78 pKa = 11.84TRR80 pKa = 11.84YY81 pKa = 9.89LYY83 pKa = 11.04LSLPDD88 pKa = 3.23SSGPINSDD96 pKa = 3.25SQKK99 pKa = 9.91SAEE102 pKa = 4.61SIRR105 pKa = 11.84KK106 pKa = 8.74SAFSRR111 pKa = 11.84IYY113 pKa = 8.94SQAVRR118 pKa = 11.84RR119 pKa = 11.84AVDD122 pKa = 4.47AIRR125 pKa = 11.84RR126 pKa = 11.84GEE128 pKa = 3.96LSTSVEE134 pKa = 4.03NVEE137 pKa = 4.26YY138 pKa = 10.18TSKK141 pKa = 9.94CTLKK145 pKa = 10.0RR146 pKa = 11.84TFEE149 pKa = 4.5SIAEE153 pKa = 4.17YY154 pKa = 10.8VPPTPFILRR163 pKa = 11.84YY164 pKa = 9.75CEE166 pKa = 3.86LFNAIFTLNLDD177 pKa = 3.55PKK179 pKa = 10.19EE180 pKa = 4.16VNEE183 pKa = 4.21EE184 pKa = 3.92GAKK187 pKa = 10.0RR188 pKa = 11.84EE189 pKa = 3.96QGKK192 pKa = 9.54RR193 pKa = 11.84CSLDD197 pKa = 3.12YY198 pKa = 11.3YY199 pKa = 10.42IGLKK203 pKa = 9.46YY204 pKa = 10.3RR205 pKa = 11.84WGRR208 pKa = 11.84PARR211 pKa = 11.84DD212 pKa = 4.29DD213 pKa = 3.01LWAYY217 pKa = 8.16QWEE220 pKa = 4.66RR221 pKa = 11.84YY222 pKa = 8.95ALNPDD227 pKa = 3.72PNDD230 pKa = 3.39PQKK233 pKa = 10.64ILRR236 pKa = 11.84EE237 pKa = 3.98HH238 pKa = 6.76RR239 pKa = 11.84EE240 pKa = 3.82GVEE243 pKa = 4.11DD244 pKa = 3.62VSRR247 pKa = 11.84TNKK250 pKa = 8.15ITFANN255 pKa = 3.77

Molecular weight: 29.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

7859

140

1334

654.9

73.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.647 ± 0.364

0.98 ± 0.142

6.006 ± 0.286

5.382 ± 0.202

4.479 ± 0.167

4.899 ± 0.229

2.239 ± 0.3

6.438 ± 0.225

4.314 ± 0.4

8.932 ± 0.372

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.227 ± 0.179

4.708 ± 0.274

5.535 ± 0.298

4.632 ± 0.263

5.84 ± 0.319

7.635 ± 0.605

6.235 ± 0.354

6.489 ± 0.254

1.107 ± 0.083

4.275 ± 0.313
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski