Tomato yellow mottle-associated virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Betarhabdovirinae; Cytorhabdovirus; Tomato yellow mottle-associated cytorhabdovirus

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U9W1C2|A0A1U9W1C2_9RHAB p3 protein OS=Tomato yellow mottle-associated virus OX=1967841 GN=P3 PE=4 SV=1
MM1 pKa = 6.91NTLALQLSNTDD12 pKa = 3.45YY13 pKa = 11.8DD14 pKa = 4.11DD15 pKa = 4.37LASITVAPGGSNVAWNDD32 pKa = 2.92EE33 pKa = 4.24DD34 pKa = 3.73VLSIRR39 pKa = 11.84RR40 pKa = 11.84YY41 pKa = 9.59SLAVMDD47 pKa = 4.56SPTMVLHH54 pKa = 6.41GGYY57 pKa = 10.14VFEE60 pKa = 4.81SLNNNARR67 pKa = 11.84VDD69 pKa = 4.12TNVLMSTVHH78 pKa = 6.5LAANLRR84 pKa = 11.84DD85 pKa = 3.89PDD87 pKa = 5.21NITQCLLTTPPHH99 pKa = 6.05SRR101 pKa = 11.84PAIIQTTLPTIRR113 pKa = 11.84AIQEE117 pKa = 4.51DD118 pKa = 3.71ITEE121 pKa = 4.23EE122 pKa = 4.02EE123 pKa = 4.7RR124 pKa = 11.84NTLALLNVDD133 pKa = 4.21VNIEE137 pKa = 4.03NQDD140 pKa = 3.52NGNQNQQGEE149 pKa = 4.64NVQVDD154 pKa = 3.52QGSIDD159 pKa = 3.73MKK161 pKa = 10.31AAAYY165 pKa = 9.92AYY167 pKa = 10.06VCAFLMRR174 pKa = 11.84LQCRR178 pKa = 11.84SAQNVSGGLRR188 pKa = 11.84RR189 pKa = 11.84AIDD192 pKa = 3.97RR193 pKa = 11.84FKK195 pKa = 10.17TWYY198 pKa = 10.13DD199 pKa = 3.0GSTDD203 pKa = 3.15IFDD206 pKa = 5.09EE207 pKa = 4.32ISFSIDD213 pKa = 2.83ALNQLKK219 pKa = 9.93EE220 pKa = 4.34AISRR224 pKa = 11.84KK225 pKa = 9.77PEE227 pKa = 3.71ITSTWVLYY235 pKa = 10.64LATTEE240 pKa = 4.19NEE242 pKa = 3.91KK243 pKa = 9.07TLLKK247 pKa = 10.15QSKK250 pKa = 10.41GMIEE254 pKa = 3.86YY255 pKa = 10.64LGLQVFSYY263 pKa = 10.79QGMHH267 pKa = 7.52ALTQVLALHH276 pKa = 5.89QMSKK280 pKa = 10.69VPLRR284 pKa = 11.84DD285 pKa = 3.19LMMEE289 pKa = 4.58LDD291 pKa = 4.03SPLTRR296 pKa = 11.84DD297 pKa = 3.49GLRR300 pKa = 11.84EE301 pKa = 3.48ISNILKK307 pKa = 9.59NHH309 pKa = 5.6EE310 pKa = 4.19RR311 pKa = 11.84TNRR314 pKa = 11.84APEE317 pKa = 3.79RR318 pKa = 11.84KK319 pKa = 7.95TYY321 pKa = 10.39FRR323 pKa = 11.84YY324 pKa = 10.21SRR326 pKa = 11.84VWDD329 pKa = 3.63TKK331 pKa = 11.22YY332 pKa = 10.27FAQLQSKK339 pKa = 7.42TCVPLLYY346 pKa = 10.2VAAVAVRR353 pKa = 11.84DD354 pKa = 3.75ISANTTSDD362 pKa = 3.05PTQIYY367 pKa = 9.94ALQNIGSAMKK377 pKa = 10.55ASLDD381 pKa = 3.44RR382 pKa = 11.84VAAALTSYY390 pKa = 10.88LVDD393 pKa = 3.49KK394 pKa = 10.54SYY396 pKa = 11.63RR397 pKa = 11.84DD398 pKa = 3.63AKK400 pKa = 10.76SGSVWDD406 pKa = 3.8AATPTEE412 pKa = 4.09

Molecular weight: 46.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U9W1C3|A0A1U9W1C3_9RHAB M protein OS=Tomato yellow mottle-associated virus OX=1967841 GN=M PE=4 SV=1
MM1 pKa = 7.72ASVFKK6 pKa = 10.82FNVKK10 pKa = 9.31TEE12 pKa = 4.11LTMSEE17 pKa = 3.92EE18 pKa = 4.42DD19 pKa = 3.83GVVSLTKK26 pKa = 10.4RR27 pKa = 11.84LNLWQTLKK35 pKa = 10.95SKK37 pKa = 9.76WMEE40 pKa = 3.69NVQLSNINFSFEE52 pKa = 4.24SRR54 pKa = 11.84GGPLAEE60 pKa = 4.09GRR62 pKa = 11.84VTASVHH68 pKa = 6.28DD69 pKa = 4.01NRR71 pKa = 11.84ISDD74 pKa = 3.93DD75 pKa = 3.74TSDD78 pKa = 3.6NTLKK82 pKa = 10.89SVTFSVTQDD91 pKa = 2.92ISFSWNYY98 pKa = 9.84NVNFEE103 pKa = 4.21VDD105 pKa = 3.48SLRR108 pKa = 11.84HH109 pKa = 5.2EE110 pKa = 4.37RR111 pKa = 11.84EE112 pKa = 3.87SPLILVTKK120 pKa = 10.58ISEE123 pKa = 4.2CNMRR127 pKa = 11.84PGYY130 pKa = 10.35SLGQIRR136 pKa = 11.84VQIEE140 pKa = 4.02MTTSDD145 pKa = 2.95KK146 pKa = 10.18MIRR149 pKa = 11.84RR150 pKa = 11.84IAKK153 pKa = 9.77SPVIKK158 pKa = 10.59LFRR161 pKa = 11.84DD162 pKa = 3.26HH163 pKa = 7.25KK164 pKa = 9.29MSEE167 pKa = 3.85NEE169 pKa = 3.65RR170 pKa = 11.84LGRR173 pKa = 11.84RR174 pKa = 11.84TSIEE178 pKa = 3.93RR179 pKa = 11.84LEE181 pKa = 4.25AAQNKK186 pKa = 9.03PLTIKK191 pKa = 10.35EE192 pKa = 4.58GPQGPYY198 pKa = 9.25MEE200 pKa = 5.89LDD202 pKa = 3.22RR203 pKa = 11.84SNSVMNIPRR212 pKa = 11.84TKK214 pKa = 10.48YY215 pKa = 10.26YY216 pKa = 10.09VAAPAKK222 pKa = 9.93EE223 pKa = 3.9II224 pKa = 3.95

Molecular weight: 25.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

3956

135

2085

565.1

64.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.207 ± 0.56

1.972 ± 0.439

6.143 ± 0.186

6.117 ± 0.385

2.932 ± 0.177

4.778 ± 0.408

2.275 ± 0.348

6.8 ± 0.489

6.143 ± 0.356

8.67 ± 0.934

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.564 ± 0.164

4.398 ± 0.461

4.019 ± 0.12

2.907 ± 0.526

6.218 ± 0.418

9.783 ± 0.585

6.269 ± 0.572

6.446 ± 0.443

1.542 ± 0.269

3.817 ± 0.461
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski