Lactococcus phage 98102
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1P8BLM4|A0A1P8BLM4_9CAUD Uncharacterized protein OS=Lactococcus phage 98102 OX=1868855 GN=DS98102_22 PE=4 SV=1
MM1 pKa = 7.43 KK2 pKa = 10.2 LSEE5 pKa = 4.33 IEE7 pKa = 4.07 AVSPEE12 pKa = 4.07 DD13 pKa = 3.31 FVVFEE18 pKa = 5.64 DD19 pKa = 3.94 DD20 pKa = 3.16 TPYY23 pKa = 11.05 SYY25 pKa = 10.77 TIDD28 pKa = 3.41 RR29 pKa = 11.84 GCRR32 pKa = 11.84 EE33 pKa = 3.83 NAIEE37 pKa = 4.64 VDD39 pKa = 4.65 DD40 pKa = 4.4 FPKK43 pKa = 10.66 LYY45 pKa = 9.59 TAEE48 pKa = 3.92 QMQEE52 pKa = 3.89 YY53 pKa = 10.56 AKK55 pKa = 10.64 ANCWEE60 pKa = 4.57 LINWYY65 pKa = 10.43 VEE67 pKa = 3.97 TTGDD71 pKa = 3.57 VNHH74 pKa = 6.68 AAEE77 pKa = 4.34 MKK79 pKa = 9.86 IWMDD83 pKa = 3.71 DD84 pKa = 3.17 EE85 pKa = 5.1 FGGHH89 pKa = 5.75 EE90 pKa = 4.15 KK91 pKa = 10.79
Molecular weight: 10.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.034
IPC2_protein 4.101
IPC_protein 4.024
Toseland 3.846
ProMoST 4.151
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.872
Rodwell 3.859
Grimsley 3.757
Solomon 3.973
Lehninger 3.923
Nozaki 4.101
DTASelect 4.24
Thurlkill 3.872
EMBOSS 3.884
Sillero 4.139
Patrickios 2.867
IPC_peptide 3.973
IPC2_peptide 4.113
IPC2.peptide.svr19 4.04
Protein with the highest isoelectric point:
>tr|A0A1P8BLP5|A0A1P8BLP5_9CAUD Tail component OS=Lactococcus phage 98102 OX=1868855 GN=DS98102_42 PE=4 SV=1
MM1 pKa = 7.33 NNEE4 pKa = 3.88 LQEE7 pKa = 3.97 LLIRR11 pKa = 11.84 IIKK14 pKa = 10.01 AAMIAIPIWGLIIMAFIIFIFKK36 pKa = 10.73 NDD38 pKa = 3.03 IKK40 pKa = 10.88 KK41 pKa = 8.34 WWRR44 pKa = 11.84 NRR46 pKa = 11.84 KK47 pKa = 8.79
Molecular weight: 5.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.156
IPC2_protein 9.589
IPC_protein 9.75
Toseland 10.935
ProMoST 10.526
Dawson 10.979
Bjellqvist 10.555
Wikipedia 11.082
Rodwell 11.506
Grimsley 10.979
Solomon 11.067
Lehninger 11.052
Nozaki 10.891
DTASelect 10.555
Thurlkill 10.891
EMBOSS 11.316
Sillero 10.891
Patrickios 11.301
IPC_peptide 11.082
IPC2_peptide 8.829
IPC2.peptide.svr19 8.698
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
53
0
53
10266
42
929
193.7
21.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.332 ± 0.409
0.701 ± 0.127
6.127 ± 0.207
7.783 ± 0.636
4.452 ± 0.194
6.322 ± 0.457
1.305 ± 0.151
6.624 ± 0.283
9.049 ± 0.439
8.095 ± 0.376
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.552 ± 0.175
6.458 ± 0.296
2.513 ± 0.167
4.257 ± 0.234
3.682 ± 0.322
6.351 ± 0.277
6.263 ± 0.275
6.078 ± 0.359
1.461 ± 0.183
3.594 ± 0.274
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here