Xanthophyllomyces dendrorhous virus L1B
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H9XW62|H9XW62_9VIRU RNA-directed RNA polymerase OS=Xanthophyllomyces dendrorhous virus L1B OX=1167691 GN=RdRp PE=3 SV=1
MM1 pKa = 6.77 LTEE4 pKa = 4.15 YY5 pKa = 10.86 VNSIVRR11 pKa = 11.84 LGNGGAFEE19 pKa = 4.23 PTLGDD24 pKa = 3.54 GRR26 pKa = 11.84 FTMVNKK32 pKa = 9.0 TRR34 pKa = 11.84 AVMEE38 pKa = 4.36 LGGVKK43 pKa = 10.02 YY44 pKa = 10.8 ASNLEE49 pKa = 4.32 LATHH53 pKa = 5.9 FQVAKK58 pKa = 10.71 AKK60 pKa = 10.32 FDD62 pKa = 3.8 VIKK65 pKa = 10.41 PVARR69 pKa = 11.84 SNFYY73 pKa = 10.78 GYY75 pKa = 10.39 NKK77 pKa = 10.25 KK78 pKa = 10.58 FIDD81 pKa = 3.78 EE82 pKa = 4.37 SGVYY86 pKa = 10.23 DD87 pKa = 4.23 PLRR90 pKa = 11.84 AIDD93 pKa = 4.02 EE94 pKa = 4.38 YY95 pKa = 11.52 SRR97 pKa = 11.84 TVPGMRR103 pKa = 11.84 LGRR106 pKa = 11.84 DD107 pKa = 3.49 DD108 pKa = 5.87 LRR110 pKa = 11.84 ALSLTDD116 pKa = 3.75 NKK118 pKa = 10.32 TDD120 pKa = 3.33 SQEE123 pKa = 3.87 AYY125 pKa = 8.92 IYY127 pKa = 11.26 NMLVSWLKK135 pKa = 10.33 ARR137 pKa = 11.84 LYY139 pKa = 11.26 VDD141 pKa = 3.92 MKK143 pKa = 11.27 GSDD146 pKa = 3.09 NKK148 pKa = 9.89 FTVKK152 pKa = 10.44 YY153 pKa = 10.69 SSFKK157 pKa = 9.33 DD158 pKa = 3.15 THH160 pKa = 7.18 VGYY163 pKa = 10.34 DD164 pKa = 3.01 INDD167 pKa = 3.48 SYY169 pKa = 11.62 GIEE172 pKa = 4.08 SVEE175 pKa = 4.38 VNLGPPNPTGEE186 pKa = 4.05 AAISLEE192 pKa = 4.56 FRR194 pKa = 11.84 DD195 pKa = 4.76 TNNFWSKK202 pKa = 10.73 PYY204 pKa = 10.14 VLKK207 pKa = 11.02 YY208 pKa = 10.63 SNNSMEE214 pKa = 3.92 QGSFYY219 pKa = 10.39 LAHH222 pKa = 6.3 VLGSNGTSGLSADD235 pKa = 3.79 IQIDD239 pKa = 3.4 ALDD242 pKa = 4.01 FNEE245 pKa = 5.14 LLLDD249 pKa = 3.87 PVGSFPTGAFNIFADD264 pKa = 5.79 FWAKK268 pKa = 10.63 PNVIWLWIMDD278 pKa = 3.86 YY279 pKa = 11.19 VRR281 pKa = 11.84 LNRR284 pKa = 11.84 VEE286 pKa = 4.31 QEE288 pKa = 3.75 FASAFEE294 pKa = 4.32 LLGALATQPLPSYY307 pKa = 10.2 HH308 pKa = 7.36 EE309 pKa = 4.76 SILWSKK315 pKa = 10.88 SRR317 pKa = 11.84 TVVNMSKK324 pKa = 10.62 FSPTRR329 pKa = 11.84 ARR331 pKa = 11.84 VPANLTGEE339 pKa = 4.45 PNVHH343 pKa = 7.11 DD344 pKa = 5.51 LNAQQFTFDD353 pKa = 4.26 EE354 pKa = 4.7 EE355 pKa = 4.14 KK356 pKa = 10.97 SPAGFITASAVLNYY370 pKa = 10.25 AFWIGIYY377 pKa = 10.93 GMVSNFAEE385 pKa = 4.85 DD386 pKa = 3.72 CSDD389 pKa = 3.04 WTDD392 pKa = 3.49 AFISSDD398 pKa = 3.62 AEE400 pKa = 4.05 LGILSTVEE408 pKa = 3.99 ARR410 pKa = 11.84 PAMISLVTGKK420 pKa = 8.07 EE421 pKa = 4.26 TNSCFSNNCFLTYY434 pKa = 10.32 DD435 pKa = 4.0 LSGMYY440 pKa = 9.56 GVKK443 pKa = 10.08 QLIVDD448 pKa = 3.95 EE449 pKa = 5.51 KK450 pKa = 10.68 IDD452 pKa = 4.06 PNHH455 pKa = 6.94 PGVILFDD462 pKa = 3.67 TVPAFVSGSLLMGAVATDD480 pKa = 3.8 YY481 pKa = 10.87 PVLKK485 pKa = 10.25 HH486 pKa = 6.39 LEE488 pKa = 3.99 PHH490 pKa = 4.55 QHH492 pKa = 5.76 IKK494 pKa = 10.32 VEE496 pKa = 4.18 RR497 pKa = 11.84 DD498 pKa = 3.02 GLLGARR504 pKa = 11.84 EE505 pKa = 4.17 AAMLANSYY513 pKa = 10.95 RR514 pKa = 11.84 LFGNDD519 pKa = 3.15 VIIEE523 pKa = 4.16 HH524 pKa = 6.09 FRR526 pKa = 11.84 SAEE529 pKa = 4.06 VYY531 pKa = 7.29 PTYY534 pKa = 11.08 ANSEE538 pKa = 4.22 EE539 pKa = 4.6 CVIATYY545 pKa = 10.82 EE546 pKa = 3.96 LFGRR550 pKa = 11.84 TRR552 pKa = 11.84 TFDD555 pKa = 2.97 IMRR558 pKa = 11.84 VSSSYY563 pKa = 10.83 KK564 pKa = 8.38 RR565 pKa = 11.84 TGRR568 pKa = 11.84 TYY570 pKa = 9.54 EE571 pKa = 4.11 IPDD574 pKa = 3.44 ATHH577 pKa = 4.98 VRR579 pKa = 11.84 TYY581 pKa = 10.93 GEE583 pKa = 4.64 CKK585 pKa = 9.48 LTMDD589 pKa = 3.96 MPVLAVCGWKK599 pKa = 9.38 QRR601 pKa = 11.84 KK602 pKa = 5.78 TVHH605 pKa = 6.1 RR606 pKa = 11.84 PKK608 pKa = 10.49 MMLDD612 pKa = 3.27 SRR614 pKa = 11.84 RR615 pKa = 11.84 VATKK619 pKa = 9.72 FMVGATSGFEE629 pKa = 3.76 KK630 pKa = 10.47 TRR632 pKa = 11.84 FAVYY636 pKa = 10.29 NRR638 pKa = 11.84 RR639 pKa = 11.84 NVMAQGFHH647 pKa = 5.4 EE648 pKa = 5.39 AKK650 pKa = 10.88 AEE652 pKa = 3.94 MAPALPLVRR661 pKa = 11.84 GSAVSTAPIEE671 pKa = 4.09 PVQEE675 pKa = 3.99 QEE677 pKa = 3.74 PANAVEE683 pKa = 4.19
Molecular weight: 76.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.373
IPC2_protein 5.41
IPC_protein 5.41
Toseland 5.601
ProMoST 5.575
Dawson 5.525
Bjellqvist 5.601
Wikipedia 5.461
Rodwell 5.474
Grimsley 5.652
Solomon 5.525
Lehninger 5.499
Nozaki 5.715
DTASelect 5.881
Thurlkill 5.715
EMBOSS 5.677
Sillero 5.804
Patrickios 4.012
IPC_peptide 5.537
IPC2_peptide 5.804
IPC2.peptide.svr19 5.761
Protein with the highest isoelectric point:
>tr|H9XW62|H9XW62_9VIRU RNA-directed RNA polymerase OS=Xanthophyllomyces dendrorhous virus L1B OX=1167691 GN=RdRp PE=3 SV=1
MM1 pKa = 7.15 TSEE4 pKa = 4.63 SVQHH8 pKa = 6.44 CEE10 pKa = 3.7 EE11 pKa = 4.32 LNGIKK16 pKa = 9.79 IIKK19 pKa = 9.07 SVRR22 pKa = 11.84 NKK24 pKa = 9.87 AMKK27 pKa = 9.44 MEE29 pKa = 4.04 MRR31 pKa = 11.84 DD32 pKa = 3.77 GYY34 pKa = 11.83 VPMCIKK40 pKa = 10.37 ARR42 pKa = 11.84 PGIVYY47 pKa = 9.41 KK48 pKa = 10.5 WEE50 pKa = 3.78 ATAFCDD56 pKa = 4.28 SEE58 pKa = 4.57 YY59 pKa = 11.42 VLVGSDD65 pKa = 3.06 MSEE68 pKa = 3.81 PSEE71 pKa = 4.11 NEE73 pKa = 3.84 SNVNIMGVLMSGLRR87 pKa = 11.84 LAGDD91 pKa = 3.48 NCTYY95 pKa = 10.97 LYY97 pKa = 11.23 AKK99 pKa = 9.77 VDD101 pKa = 3.47 QYY103 pKa = 12.05 LVTRR107 pKa = 11.84 KK108 pKa = 9.68 KK109 pKa = 10.69 AVIAIMTRR117 pKa = 11.84 HH118 pKa = 5.5 FNGLYY123 pKa = 10.67 GSVFLNDD130 pKa = 4.41 PLNMEE135 pKa = 4.06 AMFRR139 pKa = 11.84 DD140 pKa = 3.87 RR141 pKa = 11.84 APDD144 pKa = 3.74 DD145 pKa = 3.65 EE146 pKa = 5.97 LMPKK150 pKa = 9.88 QNYY153 pKa = 8.84 EE154 pKa = 4.25 KK155 pKa = 10.18 ICKK158 pKa = 9.74 KK159 pKa = 10.55 EE160 pKa = 3.81 NTKK163 pKa = 8.48 ITAQHH168 pKa = 6.64 HH169 pKa = 4.49 IHH171 pKa = 5.78 FTSEE175 pKa = 3.62 EE176 pKa = 4.19 VVRR179 pKa = 11.84 VLGKK183 pKa = 8.95 RR184 pKa = 11.84 TAEE187 pKa = 3.77 EE188 pKa = 4.3 SEE190 pKa = 4.27 ATRR193 pKa = 11.84 LPADD197 pKa = 3.38 ATMSMAAGVLLWYY210 pKa = 10.76 NEE212 pKa = 3.91 LSPVLKK218 pKa = 10.66 EE219 pKa = 4.39 MILKK223 pKa = 10.65 CGLFKK228 pKa = 11.12 SKK230 pKa = 9.6 TIQAFKK236 pKa = 10.65 KK237 pKa = 8.1 IAKK240 pKa = 9.47 DD241 pKa = 3.08 ISVEE245 pKa = 4.06 AKK247 pKa = 9.59 SLQNIVQTDD256 pKa = 3.58 LRR258 pKa = 11.84 SVFEE262 pKa = 4.61 IDD264 pKa = 3.28 TLINRR269 pKa = 11.84 IDD271 pKa = 4.25 GEE273 pKa = 4.32 VDD275 pKa = 2.53 WAEE278 pKa = 4.16 EE279 pKa = 3.62 RR280 pKa = 11.84 DD281 pKa = 3.72 HH282 pKa = 6.98 RR283 pKa = 11.84 VNPNVTNLSYY293 pKa = 11.44 SDD295 pKa = 4.08 VYY297 pKa = 11.25 DD298 pKa = 3.66 AAKK301 pKa = 10.61 DD302 pKa = 3.45 IFLQAAAVGRR312 pKa = 11.84 KK313 pKa = 7.88 PVSMDD318 pKa = 2.47 WDD320 pKa = 4.17 KK321 pKa = 11.57 YY322 pKa = 8.65 WASRR326 pKa = 11.84 WQWSAAGSIHH336 pKa = 5.44 SQYY339 pKa = 11.04 VEE341 pKa = 3.83 DD342 pKa = 4.27 DD343 pKa = 3.75 KK344 pKa = 11.97 YY345 pKa = 11.59 VIRR348 pKa = 11.84 TDD350 pKa = 3.68 RR351 pKa = 11.84 NLKK354 pKa = 10.3 NKK356 pKa = 10.12 FIAIANMPKK365 pKa = 9.93 YY366 pKa = 10.56 GYY368 pKa = 10.58 DD369 pKa = 3.04 FFMSRR374 pKa = 11.84 KK375 pKa = 7.8 PQMHH379 pKa = 6.53 AWASIKK385 pKa = 10.37 YY386 pKa = 8.37 EE387 pKa = 3.89 WGKK390 pKa = 10.34 LRR392 pKa = 11.84 AIYY395 pKa = 8.57 GTDD398 pKa = 2.92 LTSYY402 pKa = 10.13 VLSNFAFYY410 pKa = 10.89 NCEE413 pKa = 3.54 NVLPKK418 pKa = 10.28 RR419 pKa = 11.84 FPVGKK424 pKa = 9.66 DD425 pKa = 2.98 ANDD428 pKa = 3.55 ANVVNRR434 pKa = 11.84 VAGVLKK440 pKa = 10.75 DD441 pKa = 3.78 RR442 pKa = 11.84 LPYY445 pKa = 10.8 CLDD448 pKa = 3.63 FEE450 pKa = 5.44 DD451 pKa = 5.6 FNSQHH456 pKa = 6.36 SVSSMKK462 pKa = 10.66 AVIYY466 pKa = 10.14 AYY468 pKa = 10.16 GDD470 pKa = 3.32 VYY472 pKa = 10.91 RR473 pKa = 11.84 QVFTEE478 pKa = 3.96 QQLEE482 pKa = 4.1 ALAWTAEE489 pKa = 4.11 SLDD492 pKa = 3.7 DD493 pKa = 4.67 VSVNDD498 pKa = 4.18 NVGLKK503 pKa = 7.8 QTYY506 pKa = 9.64 KK507 pKa = 11.12 SNATLLSGWRR517 pKa = 11.84 LTTFVNSVLNAVYY530 pKa = 9.7 TDD532 pKa = 4.69 KK533 pKa = 11.13 ICGEE537 pKa = 4.02 AKK539 pKa = 9.81 MPGSSLHH546 pKa = 6.44 NGDD549 pKa = 4.77 DD550 pKa = 3.75 VLIGATSMKK559 pKa = 9.81 VARR562 pKa = 11.84 EE563 pKa = 3.75 SLRR566 pKa = 11.84 RR567 pKa = 11.84 SEE569 pKa = 4.65 LYY571 pKa = 10.55 GIRR574 pKa = 11.84 VQASKK579 pKa = 10.92 CAFGGIAEE587 pKa = 4.67 FLRR590 pKa = 11.84 IDD592 pKa = 3.45 HH593 pKa = 6.7 ARR595 pKa = 11.84 GSKK598 pKa = 9.72 GQYY601 pKa = 6.9 LTRR604 pKa = 11.84 AIATLMHH611 pKa = 6.29 SRR613 pKa = 11.84 IEE615 pKa = 4.5 SKK617 pKa = 10.86 LSTDD621 pKa = 3.22 ARR623 pKa = 11.84 DD624 pKa = 3.81 LIEE627 pKa = 4.82 AMEE630 pKa = 4.28 NRR632 pKa = 11.84 FSDD635 pKa = 3.83 CLNRR639 pKa = 11.84 GMTLDD644 pKa = 3.37 TVTKK648 pKa = 10.21 LRR650 pKa = 11.84 HH651 pKa = 4.95 VYY653 pKa = 10.26 YY654 pKa = 10.45 NRR656 pKa = 11.84 QSVICNMPVEE666 pKa = 4.28 DD667 pKa = 5.2 FYY669 pKa = 11.35 RR670 pKa = 11.84 IKK672 pKa = 8.18 TTHH675 pKa = 6.14 RR676 pKa = 11.84 VAGGVSEE683 pKa = 6.02 AIDD686 pKa = 3.81 SDD688 pKa = 3.67 VSMSVIRR695 pKa = 11.84 GIDD698 pKa = 2.94 KK699 pKa = 10.84 SFNIKK704 pKa = 9.61 IPKK707 pKa = 8.69 LQGVHH712 pKa = 7.11 DD713 pKa = 4.27 YY714 pKa = 11.17 ARR716 pKa = 11.84 AVAMEE721 pKa = 4.41 LEE723 pKa = 4.22 MMKK726 pKa = 10.53 RR727 pKa = 11.84 LDD729 pKa = 4.09 YY730 pKa = 10.52 ITDD733 pKa = 3.32 RR734 pKa = 11.84 MYY736 pKa = 10.98 KK737 pKa = 8.07 ATYY740 pKa = 8.66 EE741 pKa = 4.03 AVVPKK746 pKa = 9.62 TRR748 pKa = 11.84 GMTVVPNKK756 pKa = 9.42 EE757 pKa = 4.16 EE758 pKa = 3.77 KK759 pKa = 8.73 WCYY762 pKa = 8.5 NVKK765 pKa = 10.48 AIYY768 pKa = 9.86 KK769 pKa = 9.08 AFKK772 pKa = 10.75 GKK774 pKa = 9.57 IQTAGYY780 pKa = 9.69 GKK782 pKa = 10.41 AALVGMALDD791 pKa = 4.92 VIQTSDD797 pKa = 3.29 KK798 pKa = 10.87 DD799 pKa = 3.86 TTLKK803 pKa = 9.92 MALARR808 pKa = 11.84 SPDD811 pKa = 4.24 PIKK814 pKa = 10.66 LLRR817 pKa = 11.84 YY818 pKa = 9.24 LVV820 pKa = 3.53
Molecular weight: 92.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.603
IPC2_protein 7.936
IPC_protein 7.761
Toseland 7.644
ProMoST 8.331
Dawson 8.565
Bjellqvist 8.741
Wikipedia 8.565
Rodwell 8.639
Grimsley 7.688
Solomon 8.624
Lehninger 8.624
Nozaki 8.858
DTASelect 8.551
Thurlkill 8.624
EMBOSS 8.741
Sillero 8.873
Patrickios 4.368
IPC_peptide 8.624
IPC2_peptide 7.497
IPC2.peptide.svr19 7.838
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1503
683
820
751.5
84.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.649 ± 0.007
1.264 ± 0.247
6.121 ± 0.357
5.855 ± 0.189
4.125 ± 0.828
5.788 ± 0.512
1.863 ± 0.026
5.123 ± 0.468
6.321 ± 1.047
7.784 ± 0.172
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.726 ± 0.323
5.123 ± 0.188
3.593 ± 0.7
2.395 ± 0.221
5.522 ± 0.255
7.252 ± 0.231
5.522 ± 0.308
7.984 ± 0.05
1.397 ± 0.051
4.591 ± 0.127
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here