Nocardioides sp. S-1144

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4010 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B7REY2|A0A5B7REY2_9ACTN Uncharacterized protein OS=Nocardioides sp. S-1144 OX=2582905 GN=FE634_12710 PE=4 SV=1
MM1 pKa = 6.65TRR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84QRR7 pKa = 11.84VLAGTTLVSLALLAAGCGTAGKK29 pKa = 9.58IATSSEE35 pKa = 4.27PSSSAAPSSPGSSSAGEE52 pKa = 4.68DD53 pKa = 3.45PTDD56 pKa = 4.02PAPEE60 pKa = 4.78ADD62 pKa = 4.67LDD64 pKa = 4.22LALSEE69 pKa = 4.3PAEE72 pKa = 4.09DD73 pKa = 3.13SYY75 pKa = 11.8YY76 pKa = 10.44PEE78 pKa = 4.82VGDD81 pKa = 4.26PSVDD85 pKa = 3.09ALRR88 pKa = 11.84YY89 pKa = 10.23DD90 pKa = 5.85LDD92 pKa = 4.26LTWDD96 pKa = 3.97PATDD100 pKa = 3.43TLTGTEE106 pKa = 4.21TLTFRR111 pKa = 11.84STGTAEE117 pKa = 3.96EE118 pKa = 4.22FQLDD122 pKa = 3.66FAEE125 pKa = 4.46PLEE128 pKa = 4.43ITSLTLDD135 pKa = 3.65GAEE138 pKa = 3.99VDD140 pKa = 3.63HH141 pKa = 5.95EE142 pKa = 4.51HH143 pKa = 7.02RR144 pKa = 11.84GKK146 pKa = 10.54DD147 pKa = 3.69LIVSAPVEE155 pKa = 3.63EE156 pKa = 4.68DD157 pKa = 3.01ARR159 pKa = 11.84HH160 pKa = 5.34EE161 pKa = 4.08LVVEE165 pKa = 4.29YY166 pKa = 10.3SGTPAPVPAPTEE178 pKa = 4.0RR179 pKa = 11.84SDD181 pKa = 3.89LATTGFTIDD190 pKa = 5.12AEE192 pKa = 4.43HH193 pKa = 5.71QTWTMQEE200 pKa = 3.84PFGAYY205 pKa = 6.78TWYY208 pKa = 10.72AVNDD212 pKa = 3.76QPADD216 pKa = 3.3KK217 pKa = 10.71ALYY220 pKa = 10.37DD221 pKa = 3.95FTLHH225 pKa = 6.98VPDD228 pKa = 4.53PWTGVANGAKK238 pKa = 10.27VSDD241 pKa = 3.65EE242 pKa = 4.37TADD245 pKa = 3.87GTHH248 pKa = 5.52TTAFHH253 pKa = 6.31LAEE256 pKa = 4.36PAAAYY261 pKa = 9.92LVTLAFGDD269 pKa = 3.94YY270 pKa = 10.83RR271 pKa = 11.84EE272 pKa = 4.43TTDD275 pKa = 3.37VGPNGVPITYY285 pKa = 7.47WTPRR289 pKa = 11.84DD290 pKa = 3.78MEE292 pKa = 5.05PGSGLRR298 pKa = 11.84YY299 pKa = 9.91SPEE302 pKa = 3.32AMAWLEE308 pKa = 4.45TYY310 pKa = 10.54LGPYY314 pKa = 9.74PFDD317 pKa = 3.23TAGILVVDD325 pKa = 4.67SEE327 pKa = 5.18SGMEE331 pKa = 4.12TQTMITLGDD340 pKa = 3.79TEE342 pKa = 4.63YY343 pKa = 11.86SMAEE347 pKa = 3.69NTVVHH352 pKa = 6.45EE353 pKa = 5.48FAHH356 pKa = 5.24QWYY359 pKa = 8.92GDD361 pKa = 3.69QVTPDD366 pKa = 3.35DD367 pKa = 4.7WRR369 pKa = 11.84DD370 pKa = 2.74VWMNEE375 pKa = 3.02GMAMYY380 pKa = 9.61LQLMWEE386 pKa = 4.64AEE388 pKa = 4.18QSDD391 pKa = 4.58VPLDD395 pKa = 3.84SYY397 pKa = 11.93LEE399 pKa = 4.69GYY401 pKa = 9.92AGYY404 pKa = 10.38EE405 pKa = 3.48SDD407 pKa = 4.58YY408 pKa = 10.34RR409 pKa = 11.84AQSGPPADD417 pKa = 4.19YY418 pKa = 11.17DD419 pKa = 3.64PDD421 pKa = 4.02AFGSSNVYY429 pKa = 10.11FSGAFFWHH437 pKa = 7.34ALRR440 pKa = 11.84EE441 pKa = 4.25QVGDD445 pKa = 3.65EE446 pKa = 4.3VFFAAVKK453 pKa = 9.9GWPQSQDD460 pKa = 2.74NRR462 pKa = 11.84STDD465 pKa = 3.34RR466 pKa = 11.84EE467 pKa = 4.09TLLAYY472 pKa = 10.74LEE474 pKa = 4.7DD475 pKa = 3.84QTGEE479 pKa = 4.12DD480 pKa = 6.14LDD482 pKa = 4.32VLWDD486 pKa = 3.32AWLLGDD492 pKa = 4.96TSPEE496 pKa = 4.02LPP498 pKa = 3.96

Molecular weight: 54.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B7RBN2|A0A5B7RBN2_9ACTN 30S ribosomal protein S14 type Z OS=Nocardioides sp. S-1144 OX=2582905 GN=rpsZ PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.24TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4010

0

4010

1318656

33

2466

328.8

35.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.433 ± 0.048

0.708 ± 0.01

6.799 ± 0.03

5.567 ± 0.033

2.627 ± 0.022

9.522 ± 0.035

2.227 ± 0.019

2.927 ± 0.029

1.613 ± 0.028

10.337 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.655 ± 0.015

1.589 ± 0.022

5.856 ± 0.032

2.544 ± 0.016

7.974 ± 0.045

5.028 ± 0.03

6.255 ± 0.04

10.036 ± 0.042

1.474 ± 0.016

1.829 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski