Amino acid dipepetide frequency for Streptomyces sp. t39

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	23.453AlaAla: 23.453 ± 0.18	1.085AlaCys: 1.085 ± 0.022	8.677AlaAsp: 8.677 ± 0.07	8.795AlaGlu: 8.795 ± 0.067	3.549AlaPhe: 3.549 ± 0.049	14.547AlaGly: 14.547 ± 0.098	2.969AlaHis: 2.969 ± 0.043	3.084AlaIle: 3.084 ± 0.036	2.524AlaLys: 2.524 ± 0.045	14.757AlaLeu: 14.757 ± 0.11	2.572AlaMet: 2.572 ± 0.037	1.746AlaAsn: 1.746 ± 0.031	7.698AlaPro: 7.698 ± 0.082	3.638AlaGln: 3.638 ± 0.046	10.986AlaArg: 10.986 ± 0.092	6.099AlaSer: 6.099 ± 0.049	6.762AlaThr: 6.762 ± 0.066	13.031AlaVal: 13.031 ± 0.096	1.935AlaTrp: 1.935 ± 0.029	2.836AlaTyr: 2.836 ± 0.04	0.008AlaXaa: 0.008 ± 0.002
Cys	1.126CysAla: 1.126 ± 0.024	0.096CysCys: 0.096 ± 0.007	0.484CysAsp: 0.484 ± 0.014	0.403CysGlu: 0.403 ± 0.014	0.215CysPhe: 0.215 ± 0.01	0.982CysGly: 0.982 ± 0.022	0.197CysHis: 0.197 ± 0.01	0.183CysIle: 0.183 ± 0.009	0.107CysLys: 0.107 ± 0.007	0.737CysLeu: 0.737 ± 0.022	0.117CysMet: 0.117 ± 0.007	0.133CysAsn: 0.133 ± 0.008	0.522CysPro: 0.522 ± 0.017	0.14CysGln: 0.14 ± 0.009	0.664CysArg: 0.664 ± 0.016	0.443CysSer: 0.443 ± 0.015	0.531CysThr: 0.531 ± 0.015	0.64CysVal: 0.64 ± 0.018	0.115CysTrp: 0.115 ± 0.007	0.13CysTyr: 0.13 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	8.287AspAla: 8.287 ± 0.067	0.411AspCys: 0.411 ± 0.017	3.91AspAsp: 3.91 ± 0.045	4.006AspGlu: 4.006 ± 0.048	1.634AspPhe: 1.634 ± 0.028	7.055AspGly: 7.055 ± 0.07	1.399AspHis: 1.399 ± 0.023	1.864AspIle: 1.864 ± 0.034	1.04AspLys: 1.04 ± 0.026	6.102AspLeu: 6.102 ± 0.053	0.833AspMet: 0.833 ± 0.02	0.894AspAsn: 0.894 ± 0.023	4.571AspPro: 4.571 ± 0.051	1.34AspGln: 1.34 ± 0.026	5.1AspArg: 5.1 ± 0.048	2.339AspSer: 2.339 ± 0.037	3.305AspThr: 3.305 ± 0.037	4.749AspVal: 4.749 ± 0.043	1.071AspTrp: 1.071 ± 0.022	1.033AspTyr: 1.033 ± 0.024	0.002AspXaa: 0.002 ± 0.001
Glu	7.442GluAla: 7.442 ± 0.069	0.369GluCys: 0.369 ± 0.013	2.911GluAsp: 2.911 ± 0.035	3.574GluGlu: 3.574 ± 0.049	1.391GluPhe: 1.391 ± 0.026	4.434GluGly: 4.434 ± 0.055	1.502GluHis: 1.502 ± 0.026	2.23GluIle: 2.23 ± 0.033	1.402GluLys: 1.402 ± 0.027	6.579GluLeu: 6.579 ± 0.067	0.891GluMet: 0.891 ± 0.02	1.032GluAsn: 1.032 ± 0.023	3.379GluPro: 3.379 ± 0.046	2.149GluGln: 2.149 ± 0.035	5.638GluArg: 5.638 ± 0.058	2.536GluSer: 2.536 ± 0.035	2.843GluThr: 2.843 ± 0.035	4.371GluVal: 4.371 ± 0.042	0.748GluTrp: 0.748 ± 0.018	1.099GluTyr: 1.099 ± 0.025	0.002GluXaa: 0.002 ± 0.001
Phe	3.77PheAla: 3.77 ± 0.045	0.258PheCys: 0.258 ± 0.01	1.951PheAsp: 1.951 ± 0.032	1.369PheGlu: 1.369 ± 0.025	0.849PhePhe: 0.849 ± 0.025	2.941PheGly: 2.941 ± 0.039	0.6PheHis: 0.6 ± 0.018	0.614PheIle: 0.614 ± 0.019	0.441PheLys: 0.441 ± 0.017	2.538PheLeu: 2.538 ± 0.045	0.401PheMet: 0.401 ± 0.015	0.509PheAsn: 0.509 ± 0.017	1.343PhePro: 1.343 ± 0.025	0.628PheGln: 0.628 ± 0.018	1.896PheArg: 1.896 ± 0.031	1.389PheSer: 1.389 ± 0.027	2.015PheThr: 2.015 ± 0.03	2.192PheVal: 2.192 ± 0.038	0.395PheTrp: 0.395 ± 0.015	0.545PheTyr: 0.545 ± 0.013	0.0PheXaa: 0.0 ± 0.0
Gly	12.035GlyAla: 12.035 ± 0.08	0.884GlyCys: 0.884 ± 0.023	5.766GlyAsp: 5.766 ± 0.058	5.437GlyGlu: 5.437 ± 0.055	2.85GlyPhe: 2.85 ± 0.04	9.649GlyGly: 9.649 ± 0.099	2.394GlyHis: 2.394 ± 0.037	3.387GlyIle: 3.387 ± 0.044	2.143GlyLys: 2.143 ± 0.038	9.429GlyLeu: 9.429 ± 0.065	2.046GlyMet: 2.046 ± 0.033	1.705GlyAsn: 1.705 ± 0.036	5.628GlyPro: 5.628 ± 0.066	2.544GlyGln: 2.544 ± 0.033	8.693GlyArg: 8.693 ± 0.073	5.41GlySer: 5.41 ± 0.056	6.804GlyThr: 6.804 ± 0.072	7.667GlyVal: 7.667 ± 0.076	1.75GlyTrp: 1.75 ± 0.029	2.162GlyTyr: 2.162 ± 0.032	0.008GlyXaa: 0.008 ± 0.002
His	2.806HisAla: 2.806 ± 0.037	0.217HisCys: 0.217 ± 0.011	1.407HisAsp: 1.407 ± 0.024	1.205HisGlu: 1.205 ± 0.024	0.61HisPhe: 0.61 ± 0.017	2.515HisGly: 2.515 ± 0.039	0.7HisHis: 0.7 ± 0.019	0.649HisIle: 0.649 ± 0.016	0.305HisLys: 0.305 ± 0.013	2.368HisLeu: 2.368 ± 0.037	0.342HisMet: 0.342 ± 0.012	0.349HisAsn: 0.349 ± 0.013	1.854HisPro: 1.854 ± 0.028	0.591HisGln: 0.591 ± 0.014	2.263HisArg: 2.263 ± 0.031	0.965HisSer: 0.965 ± 0.023	1.358HisThr: 1.358 ± 0.024	1.73HisVal: 1.73 ± 0.03	0.379HisTrp: 0.379 ± 0.011	0.446HisTyr: 0.446 ± 0.016	0.002HisXaa: 0.002 ± 0.001
Ile	4.556IleAla: 4.556 ± 0.053	0.279IleCys: 0.279 ± 0.01	2.114IleAsp: 2.114 ± 0.033	1.857IleGlu: 1.857 ± 0.035	0.654IlePhe: 0.654 ± 0.019	3.502IleGly: 3.502 ± 0.04	0.555IleHis: 0.555 ± 0.017	0.731IleIle: 0.731 ± 0.02	0.622IleLys: 0.622 ± 0.017	2.171IleLeu: 2.171 ± 0.034	0.417IleMet: 0.417 ± 0.014	0.595IleAsn: 0.595 ± 0.017	1.605IlePro: 1.605 ± 0.03	0.617IleGln: 0.617 ± 0.016	2.211IleArg: 2.211 ± 0.031	1.486IleSer: 1.486 ± 0.029	2.132IleThr: 2.132 ± 0.032	2.641IleVal: 2.641 ± 0.038	0.336IleTrp: 0.336 ± 0.013	0.46IleTyr: 0.46 ± 0.016	0.0IleXaa: 0.0 ± 0.0
Lys	2.604LysAla: 2.604 ± 0.039	0.105LysCys: 0.105 ± 0.007	1.268LysAsp: 1.268 ± 0.025	1.124LysGlu: 1.124 ± 0.026	0.396LysPhe: 0.396 ± 0.014	1.713LysGly: 1.713 ± 0.032	0.382LysHis: 0.382 ± 0.013	0.751LysIle: 0.751 ± 0.02	0.768LysLys: 0.768 ± 0.027	1.771LysLeu: 1.771 ± 0.031	0.319LysMet: 0.319 ± 0.012	0.447LysAsn: 0.447 ± 0.016	1.206LysPro: 1.206 ± 0.029	0.627LysGln: 0.627 ± 0.015	1.328LysArg: 1.328 ± 0.028	0.952LysSer: 0.952 ± 0.022	1.089LysThr: 1.089 ± 0.025	1.687LysVal: 1.687 ± 0.033	0.235LysTrp: 0.235 ± 0.011	0.385LysTyr: 0.385 ± 0.013	0.0LysXaa: 0.0 ± 0.0
Leu	15.215LeuAla: 15.215 ± 0.103	0.834LeuCys: 0.834 ± 0.021	6.725LeuAsp: 6.725 ± 0.063	4.65LeuGlu: 4.65 ± 0.053	2.54LeuPhe: 2.54 ± 0.042	9.222LeuGly: 9.222 ± 0.071	2.327LeuHis: 2.327 ± 0.032	2.951LeuIle: 2.951 ± 0.042	1.811LeuLys: 1.811 ± 0.033	11.284LeuLeu: 11.284 ± 0.097	1.624LeuMet: 1.624 ± 0.031	1.495LeuAsn: 1.495 ± 0.029	6.469LeuPro: 6.469 ± 0.06	2.045LeuGln: 2.045 ± 0.035	8.829LeuArg: 8.829 ± 0.07	5.043LeuSer: 5.043 ± 0.051	6.715LeuThr: 6.715 ± 0.06	8.907LeuVal: 8.907 ± 0.075	1.314LeuTrp: 1.314 ± 0.029	1.793LeuTyr: 1.793 ± 0.028	0.003LeuXaa: 0.003 ± 0.001
Met	2.266MetAla: 2.266 ± 0.036	0.135MetCys: 0.135 ± 0.008	0.904MetAsp: 0.904 ± 0.025	0.798MetGlu: 0.798 ± 0.019	0.464MetPhe: 0.464 ± 0.016	1.351MetGly: 1.351 ± 0.027	0.355MetHis: 0.355 ± 0.013	0.631MetIle: 0.631 ± 0.017	0.406MetLys: 0.406 ± 0.015	1.656MetLeu: 1.656 ± 0.031	0.3MetMet: 0.3 ± 0.012	0.418MetAsn: 0.418 ± 0.013	1.136MetPro: 1.136 ± 0.021	0.442MetGln: 0.442 ± 0.014	1.477MetArg: 1.477 ± 0.026	1.318MetSer: 1.318 ± 0.026	1.531MetThr: 1.531 ± 0.026	1.259MetVal: 1.259 ± 0.025	0.191MetTrp: 0.191 ± 0.009	0.309MetTyr: 0.309 ± 0.011	0.001MetXaa: 0.001 ± 0.001
Asn	2.044AsnAla: 2.044 ± 0.031	0.152AsnCys: 0.152 ± 0.009	0.905AsnAsp: 0.905 ± 0.02	0.759AsnGlu: 0.759 ± 0.019	0.437AsnPhe: 0.437 ± 0.015	1.782AsnGly: 1.782 ± 0.034	0.375AsnHis: 0.375 ± 0.013	0.588AsnIle: 0.588 ± 0.015	0.378AsnLys: 0.378 ± 0.016	1.476AsnLeu: 1.476 ± 0.026	0.276AsnMet: 0.276 ± 0.012	0.373AsnAsn: 0.373 ± 0.015	1.217AsnPro: 1.217 ± 0.026	0.463AsnGln: 0.463 ± 0.016	1.236AsnArg: 1.236 ± 0.022	0.844AsnSer: 0.844 ± 0.022	1.069AsnThr: 1.069 ± 0.028	1.268AsnVal: 1.268 ± 0.023	0.26AsnTrp: 0.26 ± 0.011	0.369AsnTyr: 0.369 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	9.625ProAla: 9.625 ± 0.085	0.355ProCys: 0.355 ± 0.012	4.462ProAsp: 4.462 ± 0.051	4.218ProGlu: 4.218 ± 0.043	1.485ProPhe: 1.485 ± 0.027	7.304ProGly: 7.304 ± 0.066	1.386ProHis: 1.386 ± 0.026	1.098ProIle: 1.098 ± 0.021	1.006ProLys: 1.006 ± 0.024	5.401ProLeu: 5.401 ± 0.051	1.003ProMet: 1.003 ± 0.018	0.774ProAsn: 0.774 ± 0.018	3.619ProPro: 3.619 ± 0.053	1.685ProGln: 1.685 ± 0.035	4.181ProArg: 4.181 ± 0.047	3.186ProSer: 3.186 ± 0.039	2.827ProThr: 2.827 ± 0.037	5.708ProVal: 5.708 ± 0.061	0.936ProTrp: 0.936 ± 0.024	1.336ProTyr: 1.336 ± 0.022	0.004ProXaa: 0.004 ± 0.001
Gln	3.451GlnAla: 3.451 ± 0.044	0.163GlnCys: 0.163 ± 0.008	1.382GlnAsp: 1.382 ± 0.026	1.434GlnGlu: 1.434 ± 0.027	0.631GlnPhe: 0.631 ± 0.018	2.309GlnGly: 2.309 ± 0.036	0.615GlnHis: 0.615 ± 0.017	1.004GlnIle: 1.004 ± 0.024	0.575GlnLys: 0.575 ± 0.016	2.817GlnLeu: 2.817 ± 0.037	0.475GlnMet: 0.475 ± 0.015	0.448GlnAsn: 0.448 ± 0.014	1.58GlnPro: 1.58 ± 0.03	1.19GlnGln: 1.19 ± 0.034	2.243GlnArg: 2.243 ± 0.037	1.14GlnSer: 1.14 ± 0.023	1.271GlnThr: 1.271 ± 0.025	2.237GlnVal: 2.237 ± 0.033	0.449GlnTrp: 0.449 ± 0.015	0.553GlnTyr: 0.553 ± 0.017	0.001GlnXaa: 0.001 ± 0.001
Arg	10.558ArgAla: 10.558 ± 0.097	0.659ArgCys: 0.659 ± 0.018	4.529ArgAsp: 4.529 ± 0.047	4.857ArgGlu: 4.857 ± 0.057	2.356ArgPhe: 2.356 ± 0.031	6.33ArgGly: 6.33 ± 0.056	2.274ArgHis: 2.274 ± 0.035	3.351ArgIle: 3.351 ± 0.04	1.501ArgLys: 1.501 ± 0.029	9.065ArgLeu: 9.065 ± 0.087	1.777ArgMet: 1.777 ± 0.03	1.353ArgAsn: 1.353 ± 0.023	5.342ArgPro: 5.342 ± 0.057	2.256ArgGln: 2.256 ± 0.038	8.459ArgArg: 8.459 ± 0.085	4.239ArgSer: 4.239 ± 0.046	5.76ArgThr: 5.76 ± 0.059	5.95ArgVal: 5.95 ± 0.055	1.38ArgTrp: 1.38 ± 0.027	1.74ArgTyr: 1.74 ± 0.029	0.004ArgXaa: 0.004 ± 0.001
Ser	6.816SerAla: 6.816 ± 0.051	0.425SerCys: 0.425 ± 0.016	2.57SerAsp: 2.57 ± 0.035	2.197SerGlu: 2.197 ± 0.034	1.527SerPhe: 1.527 ± 0.026	5.95SerGly: 5.95 ± 0.064	0.987SerHis: 0.987 ± 0.021	1.293SerIle: 1.293 ± 0.029	0.891SerLys: 0.891 ± 0.021	4.798SerLeu: 4.798 ± 0.043	1.054SerMet: 1.054 ± 0.022	0.788SerAsn: 0.788 ± 0.017	3.149SerPro: 3.149 ± 0.044	1.2SerGln: 1.2 ± 0.023	3.747SerArg: 3.747 ± 0.047	2.692SerSer: 2.692 ± 0.045	2.828SerThr: 2.828 ± 0.037	4.236SerVal: 4.236 ± 0.047	0.934SerTrp: 0.934 ± 0.022	1.131SerTyr: 1.131 ± 0.025	0.004SerXaa: 0.004 ± 0.001
Thr	9.275ThrAla: 9.275 ± 0.075	0.434ThrCys: 0.434 ± 0.014	3.618ThrAsp: 3.618 ± 0.047	3.163ThrGlu: 3.163 ± 0.04	1.543ThrPhe: 1.543 ± 0.028	7.144ThrGly: 7.144 ± 0.062	1.185ThrHis: 1.185 ± 0.022	1.582ThrIle: 1.582 ± 0.032	0.972ThrLys: 0.972 ± 0.023	5.55ThrLeu: 5.55 ± 0.059	0.914ThrMet: 0.914 ± 0.022	0.876ThrAsn: 0.876 ± 0.022	3.967ThrPro: 3.967 ± 0.042	1.241ThrGln: 1.241 ± 0.026	4.047ThrArg: 4.047 ± 0.037	2.974ThrSer: 2.974 ± 0.037	3.769ThrThr: 3.769 ± 0.055	6.189ThrVal: 6.189 ± 0.06	0.92ThrTrp: 0.92 ± 0.022	1.31ThrTyr: 1.31 ± 0.035	0.004ThrXaa: 0.004 ± 0.001
Val	10.983ValAla: 10.983 ± 0.085	0.778ValCys: 0.778 ± 0.021	5.021ValAsp: 5.021 ± 0.051	4.679ValGlu: 4.679 ± 0.057	2.417ValPhe: 2.417 ± 0.035	6.36ValGly: 6.36 ± 0.061	2.103ValHis: 2.103 ± 0.034	2.542ValIle: 2.542 ± 0.035	1.579ValLys: 1.579 ± 0.031	9.625ValLeu: 9.625 ± 0.075	1.466ValMet: 1.466 ± 0.031	1.546ValAsn: 1.546 ± 0.023	5.521ValPro: 5.521 ± 0.054	2.021ValGln: 2.021 ± 0.03	7.538ValArg: 7.538 ± 0.064	4.315ValSer: 4.315 ± 0.048	5.76ValThr: 5.76 ± 0.057	8.157ValVal: 8.157 ± 0.08	1.166ValTrp: 1.166 ± 0.024	1.572ValTyr: 1.572 ± 0.023	0.003ValXaa: 0.003 ± 0.001
Trp	1.692TrpAla: 1.692 ± 0.032	0.15TrpCys: 0.15 ± 0.008	0.853TrpAsp: 0.853 ± 0.021	0.769TrpGlu: 0.769 ± 0.019	0.505TrpPhe: 0.505 ± 0.016	1.065TrpGly: 1.065 ± 0.021	0.382TrpHis: 0.382 ± 0.013	0.54TrpIle: 0.54 ± 0.017	0.343TrpLys: 0.343 ± 0.012	1.679TrpLeu: 1.679 ± 0.032	0.283TrpMet: 0.283 ± 0.012	0.411TrpAsn: 0.411 ± 0.014	0.789TrpPro: 0.789 ± 0.017	0.622TrpGln: 0.622 ± 0.017	1.352TrpArg: 1.352 ± 0.028	0.973TrpSer: 0.973 ± 0.024	1.12TrpThr: 1.12 ± 0.025	0.973TrpVal: 0.973 ± 0.023	0.339TrpTrp: 0.339 ± 0.014	0.356TrpTyr: 0.356 ± 0.012	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.743TyrAla: 2.743 ± 0.037	0.176TyrCys: 0.176 ± 0.009	1.488TyrAsp: 1.488 ± 0.038	1.227TyrGlu: 1.227 ± 0.022	0.587TyrPhe: 0.587 ± 0.018	2.231TyrGly: 2.231 ± 0.036	0.372TyrHis: 0.372 ± 0.012	0.467TyrIle: 0.467 ± 0.016	0.343TyrLys: 0.343 ± 0.013	1.992TyrLeu: 1.992 ± 0.032	0.235TyrMet: 0.235 ± 0.012	0.377TyrAsn: 0.377 ± 0.015	1.001TyrPro: 1.001 ± 0.025	0.527TyrGln: 0.527 ± 0.017	1.847TyrArg: 1.847 ± 0.029	0.868TyrSer: 0.868 ± 0.022	1.186TyrThr: 1.186 ± 0.026	1.557TyrVal: 1.557 ± 0.026	0.34TyrTrp: 0.34 ± 0.013	0.433TyrTyr: 0.433 ± 0.016	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.006XaaAla: 0.006 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.003XaaAsp: 0.003 ± 0.001	0.001XaaGlu: 0.001 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.009XaaGly: 0.009 ± 0.002	0.001XaaHis: 0.001 ± 0.001	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.003XaaLeu: 0.003 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.005XaaPro: 0.005 ± 0.001	0.002XaaGln: 0.002 ± 0.001	0.004XaaArg: 0.004 ± 0.001	0.002XaaSer: 0.002 ± 0.001	0.003XaaThr: 0.003 ± 0.001	0.004XaaVal: 0.004 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.005XaaXaa: 0.005 ± 0.002

Statistics based on 7058 proteins (2235208 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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