Torulaspora sp. CBS 2947

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Torulaspora; unclassified Torulaspora

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4924 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7H9HSI1|A0A7H9HSI1_9SACH Uncharacterized protein OS=Torulaspora sp. CBS 2947 OX=2792677 GN=HG537_0E00410 PE=3 SV=1
MM1 pKa = 7.55HH2 pKa = 7.7DD3 pKa = 3.31AVAVEE8 pKa = 4.28LAQTDD13 pKa = 4.08FDD15 pKa = 4.49YY16 pKa = 11.67VIAEE20 pKa = 4.13HH21 pKa = 6.48LTDD24 pKa = 3.95KK25 pKa = 10.98EE26 pKa = 4.05QFILYY31 pKa = 10.29ASEE34 pKa = 3.95QDD36 pKa = 4.33FDD38 pKa = 6.29LDD40 pKa = 3.63TCLEE44 pKa = 3.9ICEE47 pKa = 4.92GIEE50 pKa = 4.18LLNRR54 pKa = 11.84CCVEE58 pKa = 3.82EE59 pKa = 4.56RR60 pKa = 11.84SDD62 pKa = 3.57VALAEE67 pKa = 4.32SDD69 pKa = 3.97FKK71 pKa = 11.71EE72 pKa = 3.96MLQMEE77 pKa = 4.95EE78 pKa = 4.13NNRR81 pKa = 11.84IDD83 pKa = 4.06NATNDD88 pKa = 3.43LSS90 pKa = 3.64

Molecular weight: 10.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7H9I0V8|A0A7H9I0V8_9SACH Uncharacterized protein OS=Torulaspora sp. CBS 2947 OX=2792677 GN=HG537_0H02280 PE=4 SV=1
MM1 pKa = 7.33SFFVRR6 pKa = 11.84SICQLNGRR14 pKa = 11.84RR15 pKa = 11.84TLTSLSSFSPLKK27 pKa = 10.89SIGLNTNTSILGVPQLQIASLLLPFGQTLQRR58 pKa = 11.84RR59 pKa = 11.84WKK61 pKa = 10.3SRR63 pKa = 11.84GNTFQPSTLKK73 pKa = 10.07RR74 pKa = 11.84KK75 pKa = 9.56RR76 pKa = 11.84RR77 pKa = 11.84IGFLARR83 pKa = 11.84ARR85 pKa = 11.84SKK87 pKa = 10.78SGSKK91 pKa = 9.64ILQRR95 pKa = 11.84RR96 pKa = 11.84KK97 pKa = 10.45AKK99 pKa = 10.19GRR101 pKa = 11.84WFLTHH106 pKa = 7.07

Molecular weight: 12.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4924

0

4924

2385348

25

4898

484.4

54.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.102 ± 0.028

1.363 ± 0.014

5.925 ± 0.021

6.891 ± 0.036

4.285 ± 0.023

5.469 ± 0.031

2.09 ± 0.013

5.986 ± 0.024

6.723 ± 0.031

9.817 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.099 ± 0.012

5.001 ± 0.021

4.295 ± 0.025

4.152 ± 0.027

5.202 ± 0.024

8.639 ± 0.046

5.46 ± 0.033

6.151 ± 0.026

1.088 ± 0.011

3.261 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski