Amino acid dipepetide frequency for Erwinia phage ENT90

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.788AlaAla: 16.788 ± 1.748
2.087AlaCys: 2.087 ± 0.553
6.715AlaAsp: 6.715 ± 0.716
6.261AlaGlu: 6.261 ± 0.976
4.446AlaPhe: 4.446 ± 0.739
9.256AlaGly: 9.256 ± 1.362
1.996AlaHis: 1.996 ± 0.431
3.993AlaIle: 3.993 ± 0.655
4.356AlaLys: 4.356 ± 0.479
12.795AlaLeu: 12.795 ± 1.028
2.45AlaMet: 2.45 ± 0.537
2.995AlaAsn: 2.995 ± 0.494
5.898AlaPro: 5.898 ± 0.764
4.537AlaGln: 4.537 ± 0.55
6.352AlaArg: 6.352 ± 0.823
8.076AlaSer: 8.076 ± 0.969
5.626AlaThr: 5.626 ± 0.693
7.895AlaVal: 7.895 ± 0.83
1.815AlaTrp: 1.815 ± 0.326
1.906AlaTyr: 1.906 ± 0.476
0.0AlaXaa: 0.0 ± 0.0
Cys
1.633CysAla: 1.633 ± 0.5
0.363CysCys: 0.363 ± 0.215
0.454CysAsp: 0.454 ± 0.211
0.726CysGlu: 0.726 ± 0.267
0.635CysPhe: 0.635 ± 0.262
1.452CysGly: 1.452 ± 0.4
0.0CysHis: 0.0 ± 0.0
0.544CysIle: 0.544 ± 0.221
0.272CysLys: 0.272 ± 0.132
1.361CysLeu: 1.361 ± 0.45
0.817CysMet: 0.817 ± 0.261
0.363CysAsn: 0.363 ± 0.182
0.544CysPro: 0.544 ± 0.252
0.544CysGln: 0.544 ± 0.247
1.452CysArg: 1.452 ± 0.476
1.815CysSer: 1.815 ± 0.551
1.18CysThr: 1.18 ± 0.341
1.452CysVal: 1.452 ± 0.363
0.454CysTrp: 0.454 ± 0.206
0.272CysTyr: 0.272 ± 0.169
0.0CysXaa: 0.0 ± 0.0
Asp
5.445AspAla: 5.445 ± 0.683
0.635AspCys: 0.635 ± 0.23
2.269AspAsp: 2.269 ± 0.547
2.813AspGlu: 2.813 ± 0.54
2.178AspPhe: 2.178 ± 0.473
3.902AspGly: 3.902 ± 0.613
0.544AspHis: 0.544 ± 0.248
2.995AspIle: 2.995 ± 0.587
1.543AspLys: 1.543 ± 0.374
3.902AspLeu: 3.902 ± 0.6
1.815AspMet: 1.815 ± 0.529
1.543AspAsn: 1.543 ± 0.327
1.633AspPro: 1.633 ± 0.514
1.18AspGln: 1.18 ± 0.257
2.45AspArg: 2.45 ± 0.453
2.359AspSer: 2.359 ± 0.446
3.085AspThr: 3.085 ± 0.614
3.267AspVal: 3.267 ± 0.562
0.726AspTrp: 0.726 ± 0.271
1.906AspTyr: 1.906 ± 0.393
0.0AspXaa: 0.0 ± 0.0
Glu
5.898GluAla: 5.898 ± 0.75
0.726GluCys: 0.726 ± 0.27
2.722GluAsp: 2.722 ± 0.584
2.087GluGlu: 2.087 ± 0.492
1.543GluPhe: 1.543 ± 0.346
2.269GluGly: 2.269 ± 0.44
0.726GluHis: 0.726 ± 0.24
3.267GluIle: 3.267 ± 0.487
3.267GluLys: 3.267 ± 0.679
6.08GluLeu: 6.08 ± 0.69
1.996GluMet: 1.996 ± 0.597
1.724GluAsn: 1.724 ± 0.501
2.269GluPro: 2.269 ± 0.529
2.269GluGln: 2.269 ± 0.367
3.63GluArg: 3.63 ± 0.662
2.904GluSer: 2.904 ± 0.615
3.176GluThr: 3.176 ± 0.523
2.541GluVal: 2.541 ± 0.51
0.544GluTrp: 0.544 ± 0.209
1.724GluTyr: 1.724 ± 0.593
0.0GluXaa: 0.0 ± 0.0
Phe
3.448PheAla: 3.448 ± 0.621
1.18PheCys: 1.18 ± 0.391
1.543PheAsp: 1.543 ± 0.406
1.452PheGlu: 1.452 ± 0.304
1.906PhePhe: 1.906 ± 0.624
1.996PheGly: 1.996 ± 0.508
0.544PheHis: 0.544 ± 0.227
1.543PheIle: 1.543 ± 0.367
1.452PheLys: 1.452 ± 0.403
1.452PheLeu: 1.452 ± 0.373
1.18PheMet: 1.18 ± 0.296
1.452PheAsn: 1.452 ± 0.302
1.815PhePro: 1.815 ± 0.441
1.361PheGln: 1.361 ± 0.3
3.358PheArg: 3.358 ± 0.5
4.083PheSer: 4.083 ± 0.899
2.45PheThr: 2.45 ± 0.498
2.087PheVal: 2.087 ± 0.513
0.726PheTrp: 0.726 ± 0.245
0.635PheTyr: 0.635 ± 0.301
0.0PheXaa: 0.0 ± 0.0
Gly
7.441GlyAla: 7.441 ± 1.124
1.452GlyCys: 1.452 ± 0.362
3.539GlyAsp: 3.539 ± 0.68
4.265GlyGlu: 4.265 ± 0.723
2.359GlyPhe: 2.359 ± 0.417
5.445GlyGly: 5.445 ± 0.774
1.27GlyHis: 1.27 ± 0.401
2.995GlyIle: 2.995 ± 0.47
3.811GlyLys: 3.811 ± 0.568
5.989GlyLeu: 5.989 ± 0.624
1.906GlyMet: 1.906 ± 0.389
3.358GlyAsn: 3.358 ± 0.59
1.089GlyPro: 1.089 ± 0.284
2.087GlyGln: 2.087 ± 0.626
5.898GlyArg: 5.898 ± 0.61
3.811GlySer: 3.811 ± 0.675
4.083GlyThr: 4.083 ± 0.694
5.082GlyVal: 5.082 ± 0.851
0.998GlyTrp: 0.998 ± 0.32
2.087GlyTyr: 2.087 ± 0.444
0.0GlyXaa: 0.0 ± 0.0
His
2.359HisAla: 2.359 ± 0.516
0.091HisCys: 0.091 ± 0.08
0.635HisAsp: 0.635 ± 0.248
0.635HisGlu: 0.635 ± 0.224
0.998HisPhe: 0.998 ± 0.345
1.452HisGly: 1.452 ± 0.406
0.907HisHis: 0.907 ± 0.27
1.27HisIle: 1.27 ± 0.38
1.27HisLys: 1.27 ± 0.327
1.633HisLeu: 1.633 ± 0.432
0.544HisMet: 0.544 ± 0.204
0.635HisAsn: 0.635 ± 0.282
0.544HisPro: 0.544 ± 0.256
1.361HisGln: 1.361 ± 0.369
1.452HisArg: 1.452 ± 0.344
1.452HisSer: 1.452 ± 0.333
0.726HisThr: 0.726 ± 0.302
1.18HisVal: 1.18 ± 0.327
0.544HisTrp: 0.544 ± 0.227
0.635HisTyr: 0.635 ± 0.266
0.0HisXaa: 0.0 ± 0.0
Ile
5.989IleAla: 5.989 ± 0.723
1.18IleCys: 1.18 ± 0.349
2.722IleAsp: 2.722 ± 0.511
3.267IleGlu: 3.267 ± 0.53
0.907IlePhe: 0.907 ± 0.29
4.174IleGly: 4.174 ± 0.629
0.544IleHis: 0.544 ± 0.236
1.361IleIle: 1.361 ± 0.298
1.996IleLys: 1.996 ± 0.445
2.632IleLeu: 2.632 ± 0.55
0.817IleMet: 0.817 ± 0.282
2.904IleAsn: 2.904 ± 0.577
1.724IlePro: 1.724 ± 0.379
1.361IleGln: 1.361 ± 0.362
3.448IleArg: 3.448 ± 0.721
5.263IleSer: 5.263 ± 0.627
3.63IleThr: 3.63 ± 0.558
1.724IleVal: 1.724 ± 0.407
0.635IleTrp: 0.635 ± 0.255
1.27IleTyr: 1.27 ± 0.345
0.0IleXaa: 0.0 ± 0.0
Lys
5.172LysAla: 5.172 ± 0.752
0.091LysCys: 0.091 ± 0.093
1.543LysAsp: 1.543 ± 0.324
2.178LysGlu: 2.178 ± 0.532
1.633LysPhe: 1.633 ± 0.314
3.63LysGly: 3.63 ± 0.696
0.363LysHis: 0.363 ± 0.176
2.359LysIle: 2.359 ± 0.473
2.359LysLys: 2.359 ± 0.444
3.539LysLeu: 3.539 ± 0.521
0.454LysMet: 0.454 ± 0.17
1.543LysAsn: 1.543 ± 0.455
1.815LysPro: 1.815 ± 0.369
1.452LysGln: 1.452 ± 0.353
3.63LysArg: 3.63 ± 0.681
2.541LysSer: 2.541 ± 0.533
2.722LysThr: 2.722 ± 0.46
2.632LysVal: 2.632 ± 0.513
0.544LysTrp: 0.544 ± 0.252
1.452LysTyr: 1.452 ± 0.385
0.0LysXaa: 0.0 ± 0.0
Leu
10.799LeuAla: 10.799 ± 1.107
1.633LeuCys: 1.633 ± 0.381
3.358LeuAsp: 3.358 ± 0.679
3.63LeuGlu: 3.63 ± 0.583
2.904LeuPhe: 2.904 ± 0.568
3.811LeuGly: 3.811 ± 0.689
3.085LeuHis: 3.085 ± 0.595
5.263LeuIle: 5.263 ± 0.699
4.537LeuLys: 4.537 ± 0.726
7.623LeuLeu: 7.623 ± 0.759
2.632LeuMet: 2.632 ± 0.403
4.174LeuAsn: 4.174 ± 0.555
5.535LeuPro: 5.535 ± 0.832
3.902LeuGln: 3.902 ± 0.599
8.348LeuArg: 8.348 ± 0.941
5.989LeuSer: 5.989 ± 0.671
6.987LeuThr: 6.987 ± 0.855
4.265LeuVal: 4.265 ± 0.593
0.726LeuTrp: 0.726 ± 0.221
2.178LeuTyr: 2.178 ± 0.475
0.0LeuXaa: 0.0 ± 0.0
Met
3.448MetAla: 3.448 ± 0.503
0.272MetCys: 0.272 ± 0.144
0.998MetAsp: 0.998 ± 0.257
0.907MetGlu: 0.907 ± 0.299
0.817MetPhe: 0.817 ± 0.252
1.543MetGly: 1.543 ± 0.442
0.635MetHis: 0.635 ± 0.231
1.089MetIle: 1.089 ± 0.257
0.907MetLys: 0.907 ± 0.256
2.722MetLeu: 2.722 ± 0.538
1.18MetMet: 1.18 ± 0.343
1.27MetAsn: 1.27 ± 0.35
0.998MetPro: 0.998 ± 0.369
1.18MetGln: 1.18 ± 0.375
2.541MetArg: 2.541 ± 0.433
2.45MetSer: 2.45 ± 0.441
1.361MetThr: 1.361 ± 0.334
1.089MetVal: 1.089 ± 0.342
0.181MetTrp: 0.181 ± 0.138
0.363MetTyr: 0.363 ± 0.176
0.0MetXaa: 0.0 ± 0.0
Asn
3.63AsnAla: 3.63 ± 0.776
0.726AsnCys: 0.726 ± 0.197
1.906AsnAsp: 1.906 ± 0.482
2.087AsnGlu: 2.087 ± 0.357
1.18AsnPhe: 1.18 ± 0.398
3.085AsnGly: 3.085 ± 0.515
0.635AsnHis: 0.635 ± 0.317
1.543AsnIle: 1.543 ± 0.427
1.18AsnLys: 1.18 ± 0.319
3.448AsnLeu: 3.448 ± 0.542
1.089AsnMet: 1.089 ± 0.292
1.18AsnAsn: 1.18 ± 0.291
2.087AsnPro: 2.087 ± 0.503
1.633AsnGln: 1.633 ± 0.348
3.448AsnArg: 3.448 ± 0.612
2.632AsnSer: 2.632 ± 0.582
1.996AsnThr: 1.996 ± 0.521
2.45AsnVal: 2.45 ± 0.385
0.454AsnTrp: 0.454 ± 0.167
0.817AsnTyr: 0.817 ± 0.254
0.0AsnXaa: 0.0 ± 0.0
Pro
7.078ProAla: 7.078 ± 0.834
0.635ProCys: 0.635 ± 0.263
2.722ProAsp: 2.722 ± 0.532
3.085ProGlu: 3.085 ± 0.486
2.087ProPhe: 2.087 ± 0.45
2.541ProGly: 2.541 ± 0.489
1.089ProHis: 1.089 ± 0.344
1.724ProIle: 1.724 ± 0.432
1.27ProLys: 1.27 ± 0.376
4.356ProLeu: 4.356 ± 0.583
1.543ProMet: 1.543 ± 0.469
1.361ProAsn: 1.361 ± 0.441
1.996ProPro: 1.996 ± 0.373
0.726ProGln: 0.726 ± 0.277
2.359ProArg: 2.359 ± 0.49
3.358ProSer: 3.358 ± 0.692
1.906ProThr: 1.906 ± 0.425
5.989ProVal: 5.989 ± 0.775
0.635ProTrp: 0.635 ± 0.208
0.998ProTyr: 0.998 ± 0.246
0.0ProXaa: 0.0 ± 0.0
Gln
3.539GlnAla: 3.539 ± 0.6
0.726GlnCys: 0.726 ± 0.353
1.27GlnAsp: 1.27 ± 0.318
1.815GlnGlu: 1.815 ± 0.559
0.907GlnPhe: 0.907 ± 0.271
2.178GlnGly: 2.178 ± 0.455
1.18GlnHis: 1.18 ± 0.443
1.815GlnIle: 1.815 ± 0.531
1.633GlnLys: 1.633 ± 0.38
5.354GlnLeu: 5.354 ± 0.705
1.089GlnMet: 1.089 ± 0.296
1.724GlnAsn: 1.724 ± 0.356
2.178GlnPro: 2.178 ± 0.474
3.267GlnGln: 3.267 ± 0.63
3.63GlnArg: 3.63 ± 0.693
3.085GlnSer: 3.085 ± 0.431
2.359GlnThr: 2.359 ± 0.411
2.632GlnVal: 2.632 ± 0.493
0.726GlnTrp: 0.726 ± 0.249
0.817GlnTyr: 0.817 ± 0.319
0.0GlnXaa: 0.0 ± 0.0
Arg
5.445ArgAla: 5.445 ± 0.685
0.998ArgCys: 0.998 ± 0.351
3.176ArgAsp: 3.176 ± 0.499
3.63ArgGlu: 3.63 ± 0.715
1.996ArgPhe: 1.996 ± 0.414
4.083ArgGly: 4.083 ± 0.728
2.178ArgHis: 2.178 ± 0.515
4.9ArgIle: 4.9 ± 0.615
3.721ArgLys: 3.721 ± 0.526
8.167ArgLeu: 8.167 ± 0.951
1.724ArgMet: 1.724 ± 0.327
2.995ArgAsn: 2.995 ± 0.654
3.267ArgPro: 3.267 ± 0.55
5.354ArgGln: 5.354 ± 0.949
6.443ArgArg: 6.443 ± 0.966
4.446ArgSer: 4.446 ± 0.659
3.63ArgThr: 3.63 ± 0.684
6.08ArgVal: 6.08 ± 0.89
1.089ArgTrp: 1.089 ± 0.352
1.906ArgTyr: 1.906 ± 0.357
0.0ArgXaa: 0.0 ± 0.0
Ser
10.254SerAla: 10.254 ± 1.196
1.815SerCys: 1.815 ± 0.621
2.722SerAsp: 2.722 ± 0.455
3.448SerGlu: 3.448 ± 0.675
2.722SerPhe: 2.722 ± 0.639
6.08SerGly: 6.08 ± 0.942
0.544SerHis: 0.544 ± 0.364
3.176SerIle: 3.176 ± 0.492
1.724SerLys: 1.724 ± 0.498
4.9SerLeu: 4.9 ± 0.653
1.18SerMet: 1.18 ± 0.281
2.541SerAsn: 2.541 ± 0.495
3.993SerPro: 3.993 ± 0.718
2.995SerGln: 2.995 ± 0.439
5.172SerArg: 5.172 ± 0.7
6.443SerSer: 6.443 ± 1.003
5.082SerThr: 5.082 ± 0.855
5.354SerVal: 5.354 ± 0.675
1.089SerTrp: 1.089 ± 0.279
1.633SerTyr: 1.633 ± 0.459
0.0SerXaa: 0.0 ± 0.0
Thr
7.623ThrAla: 7.623 ± 1.085
0.726ThrCys: 0.726 ± 0.294
3.085ThrAsp: 3.085 ± 0.635
2.632ThrGlu: 2.632 ± 0.401
2.359ThrPhe: 2.359 ± 0.424
7.078ThrGly: 7.078 ± 0.844
1.633ThrHis: 1.633 ± 0.399
2.359ThrIle: 2.359 ± 0.44
1.906ThrLys: 1.906 ± 0.413
6.624ThrLeu: 6.624 ± 0.834
1.27ThrMet: 1.27 ± 0.29
0.726ThrAsn: 0.726 ± 0.246
4.174ThrPro: 4.174 ± 0.63
2.178ThrGln: 2.178 ± 0.371
3.448ThrArg: 3.448 ± 0.579
2.904ThrSer: 2.904 ± 0.566
2.995ThrThr: 2.995 ± 0.68
4.446ThrVal: 4.446 ± 0.751
0.544ThrTrp: 0.544 ± 0.223
1.18ThrTyr: 1.18 ± 0.289
0.0ThrXaa: 0.0 ± 0.0
Val
7.169ValAla: 7.169 ± 0.91
0.817ValCys: 0.817 ± 0.284
3.176ValAsp: 3.176 ± 0.471
4.174ValGlu: 4.174 ± 0.714
2.359ValPhe: 2.359 ± 0.466
2.632ValGly: 2.632 ± 0.449
1.633ValHis: 1.633 ± 0.463
3.358ValIle: 3.358 ± 0.509
3.176ValLys: 3.176 ± 0.584
5.445ValLeu: 5.445 ± 0.653
1.452ValMet: 1.452 ± 0.368
3.721ValAsn: 3.721 ± 0.661
3.358ValPro: 3.358 ± 0.578
2.813ValGln: 2.813 ± 0.559
4.174ValArg: 4.174 ± 0.585
5.717ValSer: 5.717 ± 0.999
4.9ValThr: 4.9 ± 0.695
3.811ValVal: 3.811 ± 0.617
0.726ValTrp: 0.726 ± 0.251
1.27ValTyr: 1.27 ± 0.375
0.0ValXaa: 0.0 ± 0.0
Trp
0.817TrpAla: 0.817 ± 0.233
0.091TrpCys: 0.091 ± 0.099
0.363TrpAsp: 0.363 ± 0.191
0.635TrpGlu: 0.635 ± 0.238
0.635TrpPhe: 0.635 ± 0.236
0.635TrpGly: 0.635 ± 0.268
0.454TrpHis: 0.454 ± 0.179
0.907TrpIle: 0.907 ± 0.32
0.635TrpLys: 0.635 ± 0.241
1.361TrpLeu: 1.361 ± 0.405
0.091TrpMet: 0.091 ± 0.094
0.544TrpAsn: 0.544 ± 0.266
0.998TrpPro: 0.998 ± 0.373
0.544TrpGln: 0.544 ± 0.186
1.815TrpArg: 1.815 ± 0.505
1.543TrpSer: 1.543 ± 0.387
0.454TrpThr: 0.454 ± 0.203
0.363TrpVal: 0.363 ± 0.158
0.181TrpTrp: 0.181 ± 0.111
0.817TrpTyr: 0.817 ± 0.223
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.269TyrAla: 2.269 ± 0.592
0.091TyrCys: 0.091 ± 0.09
1.27TyrAsp: 1.27 ± 0.347
1.633TyrGlu: 1.633 ± 0.416
1.089TyrPhe: 1.089 ± 0.404
1.724TyrGly: 1.724 ± 0.483
0.363TyrHis: 0.363 ± 0.161
1.089TyrIle: 1.089 ± 0.292
0.635TyrLys: 0.635 ± 0.192
1.996TyrLeu: 1.996 ± 0.444
0.635TyrMet: 0.635 ± 0.237
0.454TyrAsn: 0.454 ± 0.248
1.543TyrPro: 1.543 ± 0.41
1.18TyrGln: 1.18 ± 0.352
2.178TyrArg: 2.178 ± 0.466
1.996TyrSer: 1.996 ± 0.497
1.543TyrThr: 1.543 ± 0.435
1.543TyrVal: 1.543 ± 0.323
0.544TyrTrp: 0.544 ± 0.187
0.272TyrTyr: 0.272 ± 0.163
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 59 proteins (11021 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski