uncultured archaeon A07HB70

Taxonomy: cellular organisms; Archaea; environmental samples

Average proteome isoelectric point is 5.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2513 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V4XWN8|V4XWN8_9ARCH Putative metal-binding protein OS=uncultured archaeon A07HB70 OX=1412872 GN=A07HB70_01344 PE=4 SV=1
MM1 pKa = 7.44SEE3 pKa = 3.55SGYY6 pKa = 11.15AHH8 pKa = 7.39DD9 pKa = 4.86VLVSADD15 pKa = 3.37WVEE18 pKa = 3.81EE19 pKa = 3.89RR20 pKa = 11.84LDD22 pKa = 3.78EE23 pKa = 5.46FEE25 pKa = 6.01GDD27 pKa = 3.91DD28 pKa = 3.55PAHH31 pKa = 6.78RR32 pKa = 11.84LIEE35 pKa = 4.04VDD37 pKa = 3.83VDD39 pKa = 3.57TEE41 pKa = 4.49AYY43 pKa = 10.37DD44 pKa = 3.5EE45 pKa = 4.36GHH47 pKa = 6.9APGAIGFNWEE57 pKa = 4.26TQLQDD62 pKa = 3.03QTTRR66 pKa = 11.84DD67 pKa = 3.75ILSKK71 pKa = 11.2DD72 pKa = 3.53DD73 pKa = 5.11FEE75 pKa = 6.97DD76 pKa = 3.64LLGSHH81 pKa = 6.52GVSDD85 pKa = 4.52DD86 pKa = 3.81STVVLYY92 pKa = 11.02GDD94 pKa = 3.44NSNWFAAYY102 pKa = 9.84AYY104 pKa = 8.49WQFKK108 pKa = 10.54YY109 pKa = 10.53YY110 pKa = 10.93GHH112 pKa = 7.71DD113 pKa = 3.68DD114 pKa = 3.31VRR116 pKa = 11.84LLDD119 pKa = 3.73GGRR122 pKa = 11.84EE123 pKa = 3.89YY124 pKa = 10.23WVEE127 pKa = 3.7NDD129 pKa = 3.18YY130 pKa = 10.56STTDD134 pKa = 3.42EE135 pKa = 4.27VPDD138 pKa = 3.79YY139 pKa = 11.34PEE141 pKa = 5.77AEE143 pKa = 4.41YY144 pKa = 10.47EE145 pKa = 3.99ASSPRR150 pKa = 11.84EE151 pKa = 3.83SIRR154 pKa = 11.84AYY156 pKa = 10.67RR157 pKa = 11.84DD158 pKa = 3.42DD159 pKa = 3.97VEE161 pKa = 4.08NAIDD165 pKa = 3.94KK166 pKa = 9.41GIPLVDD172 pKa = 3.28VRR174 pKa = 11.84SPEE177 pKa = 4.06EE178 pKa = 3.62FSGEE182 pKa = 3.6ILAPPGLQEE191 pKa = 3.56TAQRR195 pKa = 11.84GGHH198 pKa = 6.15IPGAQNISWTSVTNDD213 pKa = 3.27DD214 pKa = 4.32GTFKK218 pKa = 10.99SADD221 pKa = 3.64EE222 pKa = 4.0LAEE225 pKa = 4.03VYY227 pKa = 10.38GDD229 pKa = 3.78HH230 pKa = 7.2GIDD233 pKa = 4.09GEE235 pKa = 4.75GTTVAYY241 pKa = 10.27CRR243 pKa = 11.84IGEE246 pKa = 4.18RR247 pKa = 11.84SSVAWFALHH256 pKa = 6.81EE257 pKa = 4.17LLGYY261 pKa = 9.22EE262 pKa = 4.87DD263 pKa = 5.55CINYY267 pKa = 10.05DD268 pKa = 3.64GSWTEE273 pKa = 3.62WGNLVGAPIEE283 pKa = 4.23TGEE286 pKa = 4.86ADD288 pKa = 3.86DD289 pKa = 4.56

Molecular weight: 32.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V4XE59|V4XE59_9ARCH Uncharacterized protein OS=uncultured archaeon A07HB70 OX=1412872 GN=A07HB70_01835 PE=4 SV=1
MM1 pKa = 7.82EE2 pKa = 5.0GRR4 pKa = 11.84EE5 pKa = 4.12LPAPPRR11 pKa = 11.84GPWRR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84GARR20 pKa = 11.84GGRR23 pKa = 11.84LGGRR27 pKa = 11.84TRR29 pKa = 11.84ARR31 pKa = 11.84PASGAGADD39 pKa = 3.48VPRR42 pKa = 11.84RR43 pKa = 11.84SRR45 pKa = 11.84ARR47 pKa = 11.84RR48 pKa = 11.84ANPLRR53 pKa = 11.84PAGGRR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84PRR62 pKa = 11.84HH63 pKa = 4.44RR64 pKa = 11.84RR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84RR69 pKa = 11.84RR70 pKa = 11.84GPLRR74 pKa = 11.84PATGCDD80 pKa = 3.34PRR82 pKa = 11.84RR83 pKa = 11.84LRR85 pKa = 11.84RR86 pKa = 11.84PARR89 pKa = 11.84RR90 pKa = 11.84PGRR93 pKa = 11.84RR94 pKa = 11.84TLGGAGLVGEE104 pKa = 4.93PPCPPGTGRR113 pKa = 11.84RR114 pKa = 11.84RR115 pKa = 11.84RR116 pKa = 11.84PGSRR120 pKa = 11.84RR121 pKa = 11.84RR122 pKa = 11.84GGARR126 pKa = 11.84RR127 pKa = 11.84PRR129 pKa = 11.84DD130 pKa = 3.31GARR133 pKa = 11.84GGAVGRR139 pKa = 11.84RR140 pKa = 11.84RR141 pKa = 11.84RR142 pKa = 11.84GPRR145 pKa = 11.84AEE147 pKa = 3.98RR148 pKa = 11.84PGRR151 pKa = 11.84GPGGPRR157 pKa = 11.84RR158 pKa = 11.84PARR161 pKa = 11.84RR162 pKa = 11.84RR163 pKa = 11.84GGRR166 pKa = 11.84RR167 pKa = 11.84RR168 pKa = 11.84AAPRR172 pKa = 11.84RR173 pKa = 11.84RR174 pKa = 11.84RR175 pKa = 11.84GRR177 pKa = 11.84VPGRR181 pKa = 11.84RR182 pKa = 11.84GARR185 pKa = 11.84RR186 pKa = 11.84VRR188 pKa = 11.84RR189 pKa = 11.84RR190 pKa = 11.84RR191 pKa = 11.84RR192 pKa = 11.84AGADD196 pKa = 2.88AGRR199 pKa = 11.84PPALPRR205 pKa = 3.9

Molecular weight: 22.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2513

0

2513

640198

24

1942

254.8

27.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.036 ± 0.088

0.749 ± 0.018

8.334 ± 0.077

7.362 ± 0.084

2.901 ± 0.036

9.212 ± 0.057

1.917 ± 0.024

2.461 ± 0.039

1.215 ± 0.024

8.96 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.517 ± 0.02

1.754 ± 0.025

5.177 ± 0.037

2.033 ± 0.03

8.197 ± 0.067

4.947 ± 0.038

6.234 ± 0.043

10.529 ± 0.061

1.01 ± 0.02

2.35 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski