Pelomonas sp. Root1444

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Pelomonas; unclassified Pelomonas

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5103 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q7SZL9|A0A0Q7SZL9_9BURK Response regulatory domain-containing protein OS=Pelomonas sp. Root1444 OX=1736464 GN=ASD35_17190 PE=4 SV=1
MM1 pKa = 7.72AAVTWTACAWPLAASAGFQWTEE23 pKa = 3.93SGPPGAGDD31 pKa = 3.81SLSTAQVTYY40 pKa = 10.63SATFEE45 pKa = 4.09TLDD48 pKa = 4.53RR49 pKa = 11.84ITGGLTASAPDD60 pKa = 3.46IKK62 pKa = 10.6GGSPIYY68 pKa = 10.3QVDD71 pKa = 3.92LFKK74 pKa = 10.9IRR76 pKa = 11.84VADD79 pKa = 3.79VSAFSAWTTGSTAFDD94 pKa = 3.59TQLYY98 pKa = 10.58LIDD101 pKa = 4.67KK102 pKa = 9.3DD103 pKa = 3.96GKK105 pKa = 10.7GVYY108 pKa = 9.86TNDD111 pKa = 4.52DD112 pKa = 3.83GLDD115 pKa = 3.77LLSLLPAASALGPLSADD132 pKa = 3.26VYY134 pKa = 10.58YY135 pKa = 10.91LAIAFGGYY143 pKa = 9.3LAQDD147 pKa = 3.27AGGASLFMGGGLTDD161 pKa = 3.63VLGGDD166 pKa = 4.0PSSGALAGWGRR177 pKa = 11.84SYY179 pKa = 11.93DD180 pKa = 3.76NGNSEE185 pKa = 5.02ALYY188 pKa = 10.13SYY190 pKa = 10.81QIGLTGATNADD201 pKa = 3.7LPEE204 pKa = 5.31PGVFMLAAVALGAAGVARR222 pKa = 11.84RR223 pKa = 11.84RR224 pKa = 11.84QSIATQAA231 pKa = 3.08

Molecular weight: 23.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q7SDQ5|A0A0Q7SDQ5_9BURK Efflux pump membrane transporter OS=Pelomonas sp. Root1444 OX=1736464 GN=ASD35_21325 PE=3 SV=1
MM1 pKa = 7.37PRR3 pKa = 11.84VGRR6 pKa = 11.84LAQIGPGPTAASLNATAGPLGMSTAAVTLPRR37 pKa = 11.84GFAISAATRR46 pKa = 11.84PSSAARR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84NAPPRR60 pKa = 11.84RR61 pKa = 11.84PGAGNAPPPAPAEE74 pKa = 4.04ARR76 pKa = 11.84SAGGG80 pKa = 3.13

Molecular weight: 7.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5103

0

5103

1742311

37

4712

341.4

36.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.792 ± 0.047

0.833 ± 0.011

5.361 ± 0.029

5.104 ± 0.03

3.362 ± 0.021

8.658 ± 0.044

2.181 ± 0.018

3.765 ± 0.024

3.013 ± 0.037

11.188 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.198 ± 0.018

2.505 ± 0.031

5.449 ± 0.031

4.002 ± 0.024

7.249 ± 0.043

5.078 ± 0.027

5.102 ± 0.033

7.431 ± 0.028

1.642 ± 0.016

2.086 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski