Escherichia phage slur01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Queuovirinae; Seuratvirus; unclassified Seuratvirus

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 90 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M7Q8U0|A0A0M7Q8U0_9CAUD Gp40 [Escherichia phage phiEB49] OS=Escherichia phage slur01 OX=1720493 PE=4 SV=1
MM1 pKa = 7.47HH2 pKa = 7.35IEE4 pKa = 4.29EE5 pKa = 4.14IVEE8 pKa = 4.07GKK10 pKa = 9.9EE11 pKa = 3.61YY12 pKa = 10.96RR13 pKa = 11.84LLTDD17 pKa = 3.43IGGVGYY23 pKa = 8.56TPDD26 pKa = 3.97DD27 pKa = 3.76AACFNVLPQGAIVTVNKK44 pKa = 9.97VVTDD48 pKa = 4.39LYY50 pKa = 10.96SVHH53 pKa = 7.46DD54 pKa = 4.51GRR56 pKa = 11.84WPHH59 pKa = 5.98AVIVTFGKK67 pKa = 10.28DD68 pKa = 3.12GYY70 pKa = 11.32VDD72 pKa = 5.34LDD74 pKa = 4.32GEE76 pKa = 4.64DD77 pKa = 3.45DD78 pKa = 4.01TYY80 pKa = 11.68EE81 pKa = 3.86VAYY84 pKa = 9.28TGLVSPNFLEE94 pKa = 4.59EE95 pKa = 4.0VV96 pKa = 3.43

Molecular weight: 10.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M7QAG5|A0A0M7QAG5_9CAUD Uncharacterized protein OS=Escherichia phage slur01 OX=1720493 PE=4 SV=1
MM1 pKa = 7.72ANLRR5 pKa = 11.84RR6 pKa = 11.84PKK8 pKa = 10.34GKK10 pKa = 9.79RR11 pKa = 11.84KK12 pKa = 8.06YY13 pKa = 7.63THH15 pKa = 5.64TGVRR19 pKa = 11.84INYY22 pKa = 8.02YY23 pKa = 10.02GEE25 pKa = 3.82NRR27 pKa = 11.84VVFLHH32 pKa = 5.82KK33 pKa = 9.44TPKK36 pKa = 9.43QWQDD40 pKa = 3.21PSGTWFNAQGKK51 pKa = 9.5QIGLYY56 pKa = 9.92GYY58 pKa = 9.53PPTCEE63 pKa = 4.84LDD65 pKa = 3.22LTTIRR70 pKa = 11.84AIEE73 pKa = 3.95DD74 pKa = 3.28RR75 pKa = 4.35

Molecular weight: 8.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

90

0

90

18497

52

1040

205.5

22.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.018 ± 0.649

1.416 ± 0.159

5.639 ± 0.198

6.488 ± 0.221

3.552 ± 0.197

7.228 ± 0.224

1.849 ± 0.156

5.839 ± 0.198

6.325 ± 0.257

7.434 ± 0.228

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.827 ± 0.168

5.055 ± 0.167

4.222 ± 0.292

4.32 ± 0.347

4.979 ± 0.177

5.477 ± 0.266

6.044 ± 0.308

7.098 ± 0.212

1.579 ± 0.131

3.611 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski