Streptococcus phage P7951
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A286QQK1|A0A286QQK1_9CAUD RecT recombinase OS=Streptococcus phage P7951 OX=1971436 GN=P7951_35 PE=4 SV=1
MM1 pKa = 7.7 EE2 pKa = 5.63 KK3 pKa = 10.43 GDD5 pKa = 3.8 YY6 pKa = 10.63 FIDD9 pKa = 3.55 VSAYY13 pKa = 10.24 QPVDD17 pKa = 3.37 LTGICQASGTNNTVIKK33 pKa = 9.37 VTEE36 pKa = 4.14 GVGWVSPVAAQQTNTSNCIGYY57 pKa = 7.92 YY58 pKa = 9.85 HH59 pKa = 7.1 FARR62 pKa = 11.84 FGGDD66 pKa = 2.81 VATAQAEE73 pKa = 4.18 ANYY76 pKa = 9.8 FISNLPSHH84 pKa = 6.85 PRR86 pKa = 11.84 FLVCDD91 pKa = 4.05 YY92 pKa = 11.23 EE93 pKa = 6.52 DD94 pKa = 4.04 GASGDD99 pKa = 3.62 KK100 pKa = 10.06 QANTNAVLAFMDD112 pKa = 3.74 VCKK115 pKa = 10.86 SEE117 pKa = 3.99 GFEE120 pKa = 4.37 PIYY123 pKa = 10.77 YY124 pKa = 9.91 SYY126 pKa = 11.36 KK127 pKa = 10.34 PYY129 pKa = 10.33 TLANVYY135 pKa = 9.91 VDD137 pKa = 4.33 QITARR142 pKa = 11.84 YY143 pKa = 7.8 PNSLWIAAYY152 pKa = 8.94 PDD154 pKa = 3.74 YY155 pKa = 10.6 EE156 pKa = 4.35 VRR158 pKa = 11.84 PEE160 pKa = 4.22 PFWDD164 pKa = 3.75 VYY166 pKa = 11.52 PNMDD170 pKa = 5.46 HH171 pKa = 6.63 IRR173 pKa = 11.84 WWQFTSTGLAGGLDD187 pKa = 3.76 KK188 pKa = 11.39 NVVIINDD195 pKa = 3.52 SDD197 pKa = 3.88 NLVDD201 pKa = 4.61 KK202 pKa = 11.26 KK203 pKa = 11.14 EE204 pKa = 4.06 EE205 pKa = 4.16 EE206 pKa = 4.12 EE207 pKa = 4.16 NMDD210 pKa = 3.47 YY211 pKa = 11.0 VVRR214 pKa = 11.84 SEE216 pKa = 4.66 SGSQGYY222 pKa = 10.01 LGVVNGHH229 pKa = 5.58 VFGISSMGTVDD240 pKa = 3.64 ALRR243 pKa = 11.84 SAGAKK248 pKa = 10.05 HH249 pKa = 6.76 LMLPDD254 pKa = 4.8 DD255 pKa = 4.25 DD256 pKa = 5.71 FEE258 pKa = 6.33 RR259 pKa = 11.84 FLNSQSNDD267 pKa = 3.01 TAQVAKK273 pKa = 10.74 AIDD276 pKa = 3.66 EE277 pKa = 4.54 ASASVVKK284 pKa = 10.68 AIEE287 pKa = 3.89 EE288 pKa = 4.05 RR289 pKa = 11.84 AQATQGQTGKK299 pKa = 10.76
Molecular weight: 32.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.194
IPC2_protein 4.418
IPC_protein 4.38
Toseland 4.202
ProMoST 4.507
Dawson 4.355
Bjellqvist 4.495
Wikipedia 4.253
Rodwell 4.215
Grimsley 4.113
Solomon 4.342
Lehninger 4.304
Nozaki 4.457
DTASelect 4.673
Thurlkill 4.228
EMBOSS 4.266
Sillero 4.495
Patrickios 2.626
IPC_peptide 4.355
IPC2_peptide 4.482
IPC2.peptide.svr19 4.423
Protein with the highest isoelectric point:
>tr|A0A286QQI5|A0A286QQI5_9CAUD Minor capsid protein OS=Streptococcus phage P7951 OX=1971436 GN=P7951_04 PE=4 SV=1
MM1 pKa = 7.83 ADD3 pKa = 3.75 FGVSLLEE10 pKa = 4.01 ARR12 pKa = 11.84 RR13 pKa = 11.84 MTLKK17 pKa = 10.49 EE18 pKa = 3.53 MKK20 pKa = 10.01 LYY22 pKa = 10.64 QKK24 pKa = 10.3 AHH26 pKa = 7.05 KK27 pKa = 10.13 KK28 pKa = 10.28 RR29 pKa = 11.84 FLNKK33 pKa = 9.47 EE34 pKa = 3.69 RR35 pKa = 11.84 EE36 pKa = 4.36 IYY38 pKa = 9.68 QLAYY42 pKa = 10.87 LNRR45 pKa = 11.84 LANATTKK52 pKa = 10.62 DD53 pKa = 3.03 GKK55 pKa = 10.64 KK56 pKa = 10.46 YY57 pKa = 10.49 YY58 pKa = 10.19 FEE60 pKa = 5.48 KK61 pKa = 10.78 FDD63 pKa = 4.29 DD64 pKa = 4.62 FYY66 pKa = 11.69 NSKK69 pKa = 9.89 EE70 pKa = 3.85 RR71 pKa = 11.84 ARR73 pKa = 11.84 EE74 pKa = 3.77 VLGEE78 pKa = 4.35 KK79 pKa = 8.61 ITNSKK84 pKa = 10.24 LLEE87 pKa = 4.07 RR88 pKa = 11.84 AKK90 pKa = 11.02 KK91 pKa = 8.79 NLNYY95 pKa = 10.35 KK96 pKa = 9.9 LEE98 pKa = 4.59 RR99 pKa = 11.84 GLLDD103 pKa = 3.37 GRR105 pKa = 4.46
Molecular weight: 12.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.075
IPC2_protein 9.341
IPC_protein 9.297
Toseland 10.072
ProMoST 9.765
Dawson 10.248
Bjellqvist 9.882
Wikipedia 10.394
Rodwell 10.774
Grimsley 10.306
Solomon 10.277
Lehninger 10.248
Nozaki 10.028
DTASelect 9.882
Thurlkill 10.101
EMBOSS 10.467
Sillero 10.145
Patrickios 10.438
IPC_peptide 10.277
IPC2_peptide 8.317
IPC2.peptide.svr19 8.348
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
47
0
47
10595
37
1545
225.4
25.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.919 ± 1.201
0.613 ± 0.127
5.89 ± 0.517
6.947 ± 0.792
4.351 ± 0.196
6.541 ± 0.529
1.387 ± 0.212
6.871 ± 0.439
7.862 ± 0.573
7.966 ± 0.327
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.445 ± 0.31
5.446 ± 0.35
2.69 ± 0.238
4.068 ± 0.268
4.068 ± 0.36
6.984 ± 0.593
6.494 ± 0.329
6.494 ± 0.298
1.085 ± 0.173
3.879 ± 0.463
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here