candidate division TM6 bacterium Zodletone_IIa

Taxonomy: cellular organisms; Bacteria; Bacteria incertae sedis; Bacteria candidate phyla; Candidatus Dependentiae; unclassified Candidatus Dependentiae

Average proteome isoelectric point is 7.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 174 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A6Y4E7|A0A0A6Y4E7_9BACT ABC transmembrane type-1 domain-containing protein OS=candidate division TM6 bacterium Zodletone_IIa OX=1511133 GN=AH06_00265 PE=3 SV=1
MM1 pKa = 7.68ANNSNEE7 pKa = 4.63LIDD10 pKa = 5.6DD11 pKa = 3.99NYY13 pKa = 10.63EE14 pKa = 4.02HH15 pKa = 7.54YY16 pKa = 11.0EE17 pKa = 3.88EE18 pKa = 5.82DD19 pKa = 3.41VDD21 pKa = 4.51GKK23 pKa = 10.9KK24 pKa = 9.94IYY26 pKa = 10.37FKK28 pKa = 10.76VAEE31 pKa = 4.21TEE33 pKa = 3.9MTKK36 pKa = 10.15QEE38 pKa = 4.04FSDD41 pKa = 4.68LSEE44 pKa = 5.07DD45 pKa = 3.33DD46 pKa = 4.48CEE48 pKa = 6.37FIFQCLLLADD58 pKa = 5.01DD59 pKa = 5.06LLKK62 pKa = 10.91KK63 pKa = 9.34HH64 pKa = 6.41TDD66 pKa = 3.58GEE68 pKa = 4.75SLNSYY73 pKa = 8.53STVTLDD79 pKa = 5.09NLIDD83 pKa = 5.27LYY85 pKa = 11.68NEE87 pKa = 4.03DD88 pKa = 4.91PKK90 pKa = 11.12IFDD93 pKa = 3.45CTKK96 pKa = 10.11NGFINSIGAAFGHH109 pKa = 6.33FLNKK113 pKa = 10.14NLGTKK118 pKa = 9.47WIVITDD124 pKa = 3.77EE125 pKa = 4.29YY126 pKa = 11.26GDD128 pKa = 4.92DD129 pKa = 4.4FSCQIDD135 pKa = 4.24EE136 pKa = 4.64LNLRR140 pKa = 11.84AFPLNSVAKK149 pKa = 10.37AIEE152 pKa = 4.03QKK154 pKa = 10.59RR155 pKa = 11.84EE156 pKa = 3.7GSLEE160 pKa = 4.11TIYY163 pKa = 11.37LLIKK167 pKa = 9.33RR168 pKa = 11.84QKK170 pKa = 10.88AEE172 pKa = 4.31LEE174 pKa = 4.18AEE176 pKa = 4.27LL177 pKa = 4.98

Molecular weight: 20.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A6VHS9|A0A0A6VHS9_9BACT Peptidase_M13 domain-containing protein OS=candidate division TM6 bacterium Zodletone_IIa OX=1511133 GN=AH06_02120 PE=4 SV=1
MM1 pKa = 7.7INRR4 pKa = 11.84LRR6 pKa = 11.84TWLLKK11 pKa = 10.63LPRR14 pKa = 11.84HH15 pKa = 6.18KK16 pKa = 10.44KK17 pKa = 10.1RR18 pKa = 11.84LIQVVADD25 pKa = 3.65VVLVFLALWMTFVVRR40 pKa = 11.84LGIDD44 pKa = 3.36EE45 pKa = 4.93MINPVKK51 pKa = 9.82MHH53 pKa = 6.71LWLFLAAPVVAIPLFIRR70 pKa = 11.84FGMYY74 pKa = 9.45RR75 pKa = 11.84AVMRR79 pKa = 11.84YY80 pKa = 8.76FGNDD84 pKa = 2.92ALVAIIKK91 pKa = 10.0AVSLSSLILGVVVYY105 pKa = 9.22WYY107 pKa = 10.79SNHH110 pKa = 5.49QNVVPPLDD118 pKa = 3.88HH119 pKa = 6.94LQLLVVKK126 pKa = 9.54LDD128 pKa = 3.11HH129 pKa = 6.66GRR131 pKa = 11.84RR132 pKa = 11.84FTLL135 pKa = 3.79

Molecular weight: 15.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

174

0

174

30796

59

802

177.0

19.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.488 ± 0.233

1.0 ± 0.081

4.793 ± 0.198

5.364 ± 0.213

5.088 ± 0.197

6.475 ± 0.18

2.166 ± 0.124

7.998 ± 0.231

7.131 ± 0.209

10.105 ± 0.243

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.611 ± 0.096

5.04 ± 0.152

4.147 ± 0.127

4.101 ± 0.164

3.744 ± 0.139

5.913 ± 0.179

5.309 ± 0.127

6.488 ± 0.168

1.237 ± 0.086

3.802 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski