False black widow spider associated circular virus 1

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346BPE8|A0A346BPE8_9VIRU Putative capsid protein OS=False black widow spider associated circular virus 1 OX=2293279 PE=4 SV=1
MM1 pKa = 7.57SRR3 pKa = 11.84GRR5 pKa = 11.84NWLFTEE11 pKa = 4.47NNPTLNPNDD20 pKa = 3.66FLEE23 pKa = 4.52SAKK26 pKa = 10.71LWDD29 pKa = 3.54HH30 pKa = 5.18VRR32 pKa = 11.84YY33 pKa = 10.0IIFQQEE39 pKa = 3.93TGEE42 pKa = 4.75GGTSHH47 pKa = 5.67YY48 pKa = 10.16QGYY51 pKa = 9.47VEE53 pKa = 4.96FSQPIRR59 pKa = 11.84FAKK62 pKa = 8.92VTGFLPRR69 pKa = 11.84AHH71 pKa = 6.55WEE73 pKa = 3.86MRR75 pKa = 11.84RR76 pKa = 11.84GTSAQCIEE84 pKa = 4.5YY85 pKa = 10.29CSKK88 pKa = 11.12LDD90 pKa = 3.65TRR92 pKa = 11.84VDD94 pKa = 4.17GPWTYY99 pKa = 11.41GDD101 pKa = 4.52PGFSSQGSRR110 pKa = 11.84SDD112 pKa = 3.23LSGAIEE118 pKa = 3.91ALRR121 pKa = 11.84EE122 pKa = 4.23GGLKK126 pKa = 10.14RR127 pKa = 11.84LRR129 pKa = 11.84EE130 pKa = 4.6DD131 pKa = 3.44CPEE134 pKa = 4.06VYY136 pKa = 10.19VKK138 pKa = 8.17YY139 pKa = 10.48HH140 pKa = 6.74RR141 pKa = 11.84GLHH144 pKa = 4.49QLYY147 pKa = 9.97NAEE150 pKa = 4.21LPSRR154 pKa = 11.84PRR156 pKa = 11.84APDD159 pKa = 3.08VHH161 pKa = 8.3LLFGPPGCGKK171 pKa = 8.28TRR173 pKa = 11.84FFYY176 pKa = 10.84DD177 pKa = 3.77NYY179 pKa = 10.13PNSPSLAVTDD189 pKa = 4.66GFWFDD194 pKa = 4.0GYY196 pKa = 10.97SGEE199 pKa = 4.75DD200 pKa = 3.0SVLIDD205 pKa = 5.2DD206 pKa = 4.74YY207 pKa = 11.67DD208 pKa = 4.06GKK210 pKa = 10.92ASRR213 pKa = 11.84WTLSNLLRR221 pKa = 11.84ILDD224 pKa = 3.96RR225 pKa = 11.84YY226 pKa = 9.88SIQVPIKK233 pKa = 10.64GGFTPWIPTRR243 pKa = 11.84IFITSNLHH251 pKa = 5.08PSNWYY256 pKa = 9.98DD257 pKa = 3.24YY258 pKa = 9.85SQRR261 pKa = 11.84QQRR264 pKa = 11.84YY265 pKa = 5.75QALSRR270 pKa = 11.84RR271 pKa = 11.84FTRR274 pKa = 11.84VTWWSSLQDD283 pKa = 4.06SEE285 pKa = 5.92DD286 pKa = 3.67LTPDD290 pKa = 3.26SPRR293 pKa = 11.84WEE295 pKa = 4.51HH296 pKa = 6.29FWAGPEE302 pKa = 4.06SAQRR306 pKa = 11.84SLDD309 pKa = 3.35IDD311 pKa = 3.58SGRR314 pKa = 11.84LISRR318 pKa = 11.84PPDD321 pKa = 3.35SEE323 pKa = 4.42FSFF326 pKa = 4.39

Molecular weight: 37.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346BPE8|A0A346BPE8_9VIRU Putative capsid protein OS=False black widow spider associated circular virus 1 OX=2293279 PE=4 SV=1
MM1 pKa = 6.97VRR3 pKa = 11.84YY4 pKa = 9.24SRR6 pKa = 11.84KK7 pKa = 9.06RR8 pKa = 11.84SFGRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84TFRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84NGLRR23 pKa = 11.84FKK25 pKa = 10.59RR26 pKa = 11.84RR27 pKa = 11.84VYY29 pKa = 10.2RR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84TFRR35 pKa = 11.84GRR37 pKa = 11.84ARR39 pKa = 11.84GMQRR43 pKa = 11.84IQRR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84YY49 pKa = 8.41PVNNPFGDD57 pKa = 3.72KK58 pKa = 10.43IFVKK62 pKa = 10.0FRR64 pKa = 11.84CTTGGNLQAPSVAAPSAGQTWNLNDD89 pKa = 4.28LGTAVGNGGAPGLEE103 pKa = 4.25EE104 pKa = 3.84YY105 pKa = 11.06SLLFKK110 pKa = 10.58RR111 pKa = 11.84YY112 pKa = 9.01RR113 pKa = 11.84ITGAKK118 pKa = 9.62VKK120 pKa = 9.04VTIYY124 pKa = 10.97NEE126 pKa = 3.82VSSDD130 pKa = 3.68VFNVDD135 pKa = 3.73PAWGFITASSDD146 pKa = 3.66AIDD149 pKa = 3.45MSDD152 pKa = 3.31YY153 pKa = 10.82HH154 pKa = 8.75LSDD157 pKa = 3.24IQHH160 pKa = 6.07LRR162 pKa = 11.84WVKK165 pKa = 10.15YY166 pKa = 10.28KK167 pKa = 10.36PLNNWTSGARR177 pKa = 11.84ATTISAYY184 pKa = 10.22FSCLKK189 pKa = 10.77VFGADD194 pKa = 2.97RR195 pKa = 11.84TIPDD199 pKa = 4.39DD200 pKa = 4.37LDD202 pKa = 3.58YY203 pKa = 11.04TATTNSAGTPFYY215 pKa = 8.67NTPSITAGVGWGVVKK230 pKa = 10.85ANASNWGASEE240 pKa = 5.29SIGQYY245 pKa = 7.44TQTVTYY251 pKa = 9.7YY252 pKa = 10.73AQLWDD257 pKa = 4.09PEE259 pKa = 4.37LGQTNVV265 pKa = 2.94

Molecular weight: 29.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

591

265

326

295.5

33.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.922 ± 1.662

1.015 ± 0.182

6.091 ± 0.828

3.723 ± 1.282

5.245 ± 0.237

8.46 ± 0.416

1.692 ± 0.654

4.23 ± 0.055

3.215 ± 0.654

6.937 ± 1.155

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

0.846 ± 0.2

4.569 ± 1.026

5.753 ± 1.119

3.892 ± 0.346

9.645 ± 0.643

8.799 ± 1.137

6.768 ± 1.334

4.907 ± 1.316

3.215 ± 0.4

5.076 ± 0.144
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski