Rariglobus hedericola
Average proteome isoelectric point is 6.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3424 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A556QM92|A0A556QM92_9BACT 30S ribosomal protein S21 OS=Rariglobus hedericola OX=2597822 GN=rpsU PE=3 SV=1
MM1 pKa = 7.18 NVIRR5 pKa = 11.84 LLFSSVFLAAATSLSALTIDD25 pKa = 4.29 FFDD28 pKa = 4.17 VNTSTEE34 pKa = 4.05 SGPAQSYY41 pKa = 8.11 EE42 pKa = 4.08 GPGNGVYY49 pKa = 10.71 YY50 pKa = 10.44 YY51 pKa = 10.94 GSDD54 pKa = 3.29 SAGGFTSGGAQFVNYY69 pKa = 9.85 YY70 pKa = 10.32 NSDD73 pKa = 3.13 WGSWSGWAYY82 pKa = 9.69 STTADD87 pKa = 3.7 SVTAGMANQYY97 pKa = 10.69 SAFPGALTTGTPYY110 pKa = 11.08 GIAYY114 pKa = 9.21 VSSYY118 pKa = 11.17 DD119 pKa = 3.5 GPTTITLPAGIDD131 pKa = 3.59 TPLSLTLTNTTYY143 pKa = 10.88 AYY145 pKa = 10.86 DD146 pKa = 3.43 SMLNGDD152 pKa = 4.12 SFAKK156 pKa = 10.48 KK157 pKa = 10.15 FGGVSGNDD165 pKa = 3.21 ADD167 pKa = 4.14 WFLLTITGKK176 pKa = 10.3 DD177 pKa = 3.2 AFNATLGSVEE187 pKa = 5.01 FYY189 pKa = 11.0 LADD192 pKa = 4.15 FRR194 pKa = 11.84 FADD197 pKa = 3.69 NEE199 pKa = 3.87 LDD201 pKa = 4.41 YY202 pKa = 10.98 IITDD206 pKa = 3.32 WTDD209 pKa = 2.51 VDD211 pKa = 4.21 LTGLGTGVHH220 pKa = 5.28 TLEE223 pKa = 4.3 FTLTSSDD230 pKa = 3.47 SGDD233 pKa = 3.43 YY234 pKa = 11.44 GMNTPSYY241 pKa = 9.05 FALGGVTAVPEE252 pKa = 4.21 PSAYY256 pKa = 10.02 AALVGAFTLVLALTRR271 pKa = 11.84 RR272 pKa = 11.84 RR273 pKa = 11.84 RR274 pKa = 11.84 SS275 pKa = 3.13
Molecular weight: 29.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.845
IPC2_protein 3.77
IPC_protein 3.783
Toseland 3.541
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.77
Rodwell 3.605
Grimsley 3.452
Solomon 3.783
Lehninger 3.745
Nozaki 3.91
DTASelect 4.202
Thurlkill 3.617
EMBOSS 3.77
Sillero 3.91
Patrickios 0.134
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.814
Protein with the highest isoelectric point:
>tr|A0A556QJA4|A0A556QJA4_9BACT Response regulator OS=Rariglobus hedericola OX=2597822 GN=FPL22_11340 PE=4 SV=1
MM1 pKa = 7.15 QPTFRR6 pKa = 11.84 PHH8 pKa = 5.7 RR9 pKa = 11.84 LKK11 pKa = 10.64 RR12 pKa = 11.84 ARR14 pKa = 11.84 KK15 pKa = 8.44 IGFFARR21 pKa = 11.84 KK22 pKa = 7.88 ATVGGRR28 pKa = 11.84 KK29 pKa = 9.08 VLANRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.79 GRR39 pKa = 11.84 KK40 pKa = 8.81 RR41 pKa = 11.84 LTVVV45 pKa = 3.08
Molecular weight: 5.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3424
0
3424
1258522
22
9126
367.6
40.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.409 ± 0.053
0.857 ± 0.014
5.165 ± 0.032
5.236 ± 0.049
4.044 ± 0.024
7.982 ± 0.057
2.117 ± 0.028
5.156 ± 0.033
3.954 ± 0.041
10.342 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.852 ± 0.021
3.268 ± 0.039
5.215 ± 0.037
3.173 ± 0.022
6.335 ± 0.05
6.225 ± 0.047
6.411 ± 0.079
7.248 ± 0.028
1.533 ± 0.027
2.478 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here