Lignipirellula cremea
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6917 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A518DQY0|A0A518DQY0_9BACT Adenine phosphoribosyltransferase OS=Lignipirellula cremea OX=2528010 GN=apt PE=3 SV=1
MM1 pKa = 7.59 ASSPFDD7 pKa = 3.68 NDD9 pKa = 2.64 SDD11 pKa = 4.02 IPADD15 pKa = 3.56 SALDD19 pKa = 3.59 MDD21 pKa = 5.31 LGGDD25 pKa = 3.67 MSLDD29 pKa = 3.42 MPVEE33 pKa = 4.11 STEE36 pKa = 4.17 NAEE39 pKa = 4.07 QAPGDD44 pKa = 3.98 MFAGVPDD51 pKa = 4.01 KK52 pKa = 11.38 APAGGPAEE60 pKa = 4.27 ADD62 pKa = 3.27 APEE65 pKa = 4.32 EE66 pKa = 4.34 VPVKK70 pKa = 9.76 RR71 pKa = 11.84 TPSDD75 pKa = 3.63 VYY77 pKa = 11.23 SWLLAAAFICLCTGVLLLYY96 pKa = 10.12 MEE98 pKa = 4.54 LRR100 pKa = 11.84 DD101 pKa = 3.99 YY102 pKa = 11.55 GSYY105 pKa = 10.39 PYY107 pKa = 10.14 WKK109 pKa = 8.56 TSGSAAVGASGRR121 pKa = 3.8
Molecular weight: 12.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.706
IPC_protein 3.681
Toseland 3.465
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.643
Rodwell 3.503
Grimsley 3.376
Solomon 3.656
Lehninger 3.617
Nozaki 3.808
DTASelect 4.037
Thurlkill 3.528
EMBOSS 3.643
Sillero 3.795
Patrickios 1.863
IPC_peptide 3.656
IPC2_peptide 3.77
IPC2.peptide.svr19 3.757
Protein with the highest isoelectric point:
>tr|A0A518DRY8|A0A518DRY8_9BACT Uncharacterized protein OS=Lignipirellula cremea OX=2528010 GN=Pla8534_23860 PE=4 SV=1
MM1 pKa = 7.18 RR2 pKa = 11.84 ASWNRR7 pKa = 11.84 CVEE10 pKa = 4.28 RR11 pKa = 11.84 AASPANCRR19 pKa = 11.84 PTCVMRR25 pKa = 11.84 RR26 pKa = 11.84 PIRR29 pKa = 11.84 RR30 pKa = 11.84 IRR32 pKa = 11.84 LVATRR37 pKa = 11.84 LPQAPRR43 pKa = 11.84 PFRR46 pKa = 11.84 ILDD49 pKa = 3.49 RR50 pKa = 11.84 LAIPLPPPPSRR61 pKa = 11.84 LRR63 pKa = 11.84 TIRR66 pKa = 11.84 SATPLLPLRR75 pKa = 11.84 LLLLSLRR82 pKa = 11.84 TTIRR86 pKa = 11.84 SALLPAILRR95 pKa = 11.84 PPRR98 pKa = 11.84 LFPRR102 pKa = 11.84 TTIRR106 pKa = 11.84 SVLLPALPPVTRR118 pKa = 11.84 LLPLVMIRR126 pKa = 11.84 SAPPPAILRR135 pKa = 11.84 PPRR138 pKa = 11.84 LAMIRR143 pKa = 11.84 SVLLPAILRR152 pKa = 11.84 PPRR155 pKa = 11.84 LAMIRR160 pKa = 11.84 SVHH163 pKa = 6.26 PLALPPVTRR172 pKa = 11.84 LLPLVMIRR180 pKa = 11.84 SVLPPAIPRR189 pKa = 11.84 PLLLATIRR197 pKa = 11.84 LALLLAIPRR206 pKa = 11.84 PPRR209 pKa = 11.84 LATIRR214 pKa = 11.84 LAHH217 pKa = 7.04 LLALLPAPRR226 pKa = 11.84 LLRR229 pKa = 11.84 LATIRR234 pKa = 11.84 SAPPLAILRR243 pKa = 11.84 PPRR246 pKa = 11.84 LATIRR251 pKa = 11.84 LAHH254 pKa = 6.81 LPALPPVTRR263 pKa = 11.84 LLPLVMIRR271 pKa = 11.84 SVLPPAILRR280 pKa = 11.84 PLRR283 pKa = 11.84 LATIRR288 pKa = 11.84 LAHH291 pKa = 7.04 LLALLPAPRR300 pKa = 11.84 LLRR303 pKa = 11.84 LAMIRR308 pKa = 11.84 SAPPPAILRR317 pKa = 11.84 PLRR320 pKa = 11.84 LATIRR325 pKa = 11.84 LAHH328 pKa = 7.03 LLALLLVTRR337 pKa = 11.84 PLRR340 pKa = 11.84 LALIRR345 pKa = 11.84 SAPLPLLRR353 pKa = 11.84 LAPLPLIRR361 pKa = 11.84 LAPLPLIRR369 pKa = 11.84 SAPLLLLRR377 pKa = 11.84 RR378 pKa = 11.84 PLPPVGIRR386 pKa = 11.84 LAMTRR391 pKa = 11.84 LAPIRR396 pKa = 11.84 LPRR399 pKa = 11.84 LRR401 pKa = 11.84 RR402 pKa = 11.84 PHH404 pKa = 6.49 RR405 pKa = 11.84 RR406 pKa = 11.84 LPRR409 pKa = 11.84 PLLRR413 pKa = 11.84 LRR415 pKa = 11.84 RR416 pKa = 3.89
Molecular weight: 46.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.286
IPC_protein 12.896
Toseland 13.056
ProMoST 13.568
Dawson 13.056
Bjellqvist 13.056
Wikipedia 13.539
Rodwell 12.559
Grimsley 13.1
Solomon 13.554
Lehninger 13.466
Nozaki 13.056
DTASelect 13.056
Thurlkill 13.056
EMBOSS 13.554
Sillero 13.056
Patrickios 12.266
IPC_peptide 13.568
IPC2_peptide 12.559
IPC2.peptide.svr19 9.256
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6917
0
6917
2670640
29
7286
386.1
42.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.893 ± 0.038
1.152 ± 0.012
5.845 ± 0.023
6.291 ± 0.026
3.655 ± 0.014
7.748 ± 0.03
2.208 ± 0.013
4.269 ± 0.021
3.434 ± 0.023
10.472 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.073 ± 0.013
2.845 ± 0.017
5.866 ± 0.027
4.478 ± 0.025
6.9 ± 0.031
5.854 ± 0.022
5.145 ± 0.023
6.814 ± 0.022
1.566 ± 0.013
2.49 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here