Tenacibaculum phage PTm1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Shirahamavirus; Tenacibaculum virus PTm1

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 308 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5S9HXG4|A0A5S9HXG4_9CAUD Uncharacterized protein OS=Tenacibaculum phage PTm1 OX=2547425 PE=4 SV=1
MM1 pKa = 8.21DD2 pKa = 5.0IYY4 pKa = 11.52DD5 pKa = 4.77EE6 pKa = 4.51IGQVDD11 pKa = 3.98IEE13 pKa = 4.38DD14 pKa = 4.81LSDD17 pKa = 3.43EE18 pKa = 4.18MQEE21 pKa = 4.27KK22 pKa = 9.25IGYY25 pKa = 8.47MGMLVSALYY34 pKa = 9.94ICGSISFVILMLKK47 pKa = 10.15IIIKK51 pKa = 10.0IYY53 pKa = 10.82SNN55 pKa = 3.52

Molecular weight: 6.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5S9HXC3|A0A5S9HXC3_9CAUD Uncharacterized protein OS=Tenacibaculum phage PTm1 OX=2547425 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.12NLKK5 pKa = 10.55NIDD8 pKa = 3.4SRR10 pKa = 11.84KK11 pKa = 9.66LVSSTLFDD19 pKa = 3.88VKK21 pKa = 11.01DD22 pKa = 3.38GDD24 pKa = 4.1TFHH27 pKa = 6.65TKK29 pKa = 8.92SMRR32 pKa = 11.84ITGFGTTWKK41 pKa = 10.04VIKK44 pKa = 8.88VTPKK48 pKa = 10.62LMEE51 pKa = 4.24LPRR54 pKa = 11.84INYY57 pKa = 7.78YY58 pKa = 10.84KK59 pKa = 10.41NPKK62 pKa = 9.01NKK64 pKa = 9.63TYY66 pKa = 10.38TDD68 pKa = 3.62RR69 pKa = 11.84NPKK72 pKa = 8.96VRR74 pKa = 11.84CNNCNRR80 pKa = 11.84PIKK83 pKa = 10.49QNVIDD88 pKa = 4.62RR89 pKa = 11.84KK90 pKa = 10.56QITPWFCYY98 pKa = 9.21HH99 pKa = 7.07CYY101 pKa = 10.24LRR103 pKa = 11.84LVKK106 pKa = 10.4GYY108 pKa = 11.0LNITITRR115 pKa = 11.84KK116 pKa = 10.34KK117 pKa = 10.55NGLTKK122 pKa = 10.25TIHH125 pKa = 6.55LPTKK129 pKa = 10.3IEE131 pKa = 4.06LNKK134 pKa = 10.68KK135 pKa = 9.18KK136 pKa = 10.27FRR138 pKa = 11.84RR139 pKa = 3.77

Molecular weight: 16.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

308

0

308

66768

37

1336

216.8

24.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.248 ± 0.151

0.98 ± 0.063

6.365 ± 0.109

6.464 ± 0.195

4.637 ± 0.106

5.016 ± 0.165

1.926 ± 0.072

7.829 ± 0.119

8.983 ± 0.192

8.342 ± 0.129

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.289 ± 0.067

7.256 ± 0.106

2.756 ± 0.083

3.296 ± 0.087

3.768 ± 0.09

6.993 ± 0.142

6.807 ± 0.197

6.277 ± 0.108

0.98 ± 0.041

4.788 ± 0.124
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski