Brome mosaic virus (BMV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Bromoviridae; Bromovirus

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P03602|CAPSD_BMV Capsid protein OS=Brome mosaic virus OX=12302 GN=ORF3b PE=1 SV=1
MM1 pKa = 7.84SSKK4 pKa = 8.82TWDD7 pKa = 3.48DD8 pKa = 3.54DD9 pKa = 3.75FVRR12 pKa = 11.84QVPSFQWIIDD22 pKa = 3.48QSLEE26 pKa = 4.29DD27 pKa = 3.89EE28 pKa = 4.67VEE30 pKa = 3.99AASLQVQEE38 pKa = 4.79PADD41 pKa = 3.71GVAIDD46 pKa = 4.57GSLASFKK53 pKa = 10.84LAIAPLEE60 pKa = 4.11IGGVFDD66 pKa = 4.38PPFDD70 pKa = 3.66RR71 pKa = 11.84VRR73 pKa = 11.84WGSICDD79 pKa = 3.54TVQQMVQQFTDD90 pKa = 3.43RR91 pKa = 11.84PLIPQAEE98 pKa = 4.68MARR101 pKa = 11.84MLYY104 pKa = 10.58LDD106 pKa = 3.75IPGSFVLEE114 pKa = 4.3DD115 pKa = 5.44EE116 pKa = 4.4IDD118 pKa = 3.22DD119 pKa = 4.41WYY121 pKa = 10.61PEE123 pKa = 4.19DD124 pKa = 3.63TSDD127 pKa = 4.16GYY129 pKa = 11.5GVSFAADD136 pKa = 3.54EE137 pKa = 4.32DD138 pKa = 4.23HH139 pKa = 7.85ASDD142 pKa = 5.12LKK144 pKa = 11.1LASDD148 pKa = 4.15SSNCEE153 pKa = 3.48IEE155 pKa = 4.34EE156 pKa = 3.99VRR158 pKa = 11.84VTGDD162 pKa = 3.25TPKK165 pKa = 10.81EE166 pKa = 3.76LTLGDD171 pKa = 3.74RR172 pKa = 11.84YY173 pKa = 10.38MGIDD177 pKa = 4.16EE178 pKa = 4.86EE179 pKa = 4.57FQTTNTDD186 pKa = 2.9YY187 pKa = 11.31DD188 pKa = 3.39ITLQIMNPIEE198 pKa = 4.09HH199 pKa = 6.3RR200 pKa = 11.84VSRR203 pKa = 11.84VIDD206 pKa = 3.6THH208 pKa = 6.04CHH210 pKa = 6.27PDD212 pKa = 3.36NPDD215 pKa = 2.87ISTGPIYY222 pKa = 9.77MEE224 pKa = 3.97RR225 pKa = 11.84VSLARR230 pKa = 11.84TEE232 pKa = 4.27ATSHH236 pKa = 6.62SILPTHH242 pKa = 7.35AYY244 pKa = 10.36FDD246 pKa = 4.38DD247 pKa = 4.87SYY249 pKa = 11.63HH250 pKa = 5.86QALVEE255 pKa = 4.22NGDD258 pKa = 3.4YY259 pKa = 11.59SMDD262 pKa = 3.65FDD264 pKa = 6.27RR265 pKa = 11.84IRR267 pKa = 11.84LKK269 pKa = 10.82QSDD272 pKa = 4.15VDD274 pKa = 3.6WYY276 pKa = 11.02RR277 pKa = 11.84DD278 pKa = 3.3PDD280 pKa = 3.78KK281 pKa = 11.4YY282 pKa = 10.76FQPKK286 pKa = 8.96MNIGSAQRR294 pKa = 11.84RR295 pKa = 11.84VGTQKK300 pKa = 10.68EE301 pKa = 4.29VLTALKK307 pKa = 10.1KK308 pKa = 10.58RR309 pKa = 11.84NADD312 pKa = 3.46VPEE315 pKa = 4.39MGDD318 pKa = 3.95AINMKK323 pKa = 9.12DD324 pKa = 3.25TAKK327 pKa = 10.79AIAKK331 pKa = 9.48RR332 pKa = 11.84FRR334 pKa = 11.84STFLNVDD341 pKa = 3.39GEE343 pKa = 4.57DD344 pKa = 3.82CLRR347 pKa = 11.84ASMDD351 pKa = 3.27VMTKK355 pKa = 10.22CLEE358 pKa = 4.03YY359 pKa = 9.85HH360 pKa = 6.25KK361 pKa = 10.73KK362 pKa = 8.54WGKK365 pKa = 10.03HH366 pKa = 4.31MDD368 pKa = 3.53LQGVNVAAEE377 pKa = 4.21TDD379 pKa = 3.45LCRR382 pKa = 11.84YY383 pKa = 7.52QHH385 pKa = 5.81MLKK388 pKa = 10.29SDD390 pKa = 3.81VKK392 pKa = 10.66PVVTDD397 pKa = 3.44TLHH400 pKa = 7.33LEE402 pKa = 4.03RR403 pKa = 11.84AVAATITFHH412 pKa = 7.15SKK414 pKa = 10.29GVTSNFSPFFTACFEE429 pKa = 4.28KK430 pKa = 10.91LSLALKK436 pKa = 10.44SRR438 pKa = 11.84FIVPIGKK445 pKa = 9.47ISSLEE450 pKa = 4.01LKK452 pKa = 10.3NVRR455 pKa = 11.84LNNRR459 pKa = 11.84YY460 pKa = 8.9FLEE463 pKa = 5.12ADD465 pKa = 3.35LSKK468 pKa = 10.63FDD470 pKa = 3.7KK471 pKa = 11.18SQGEE475 pKa = 3.98LHH477 pKa = 7.06LEE479 pKa = 4.13FQRR482 pKa = 11.84EE483 pKa = 3.73ILLALGFPAPLTNWWSDD500 pKa = 4.21FHH502 pKa = 7.43RR503 pKa = 11.84DD504 pKa = 3.28SYY506 pKa = 11.67LSDD509 pKa = 3.12PHH511 pKa = 8.2AKK513 pKa = 10.16VGMSVSFQRR522 pKa = 11.84RR523 pKa = 11.84TGDD526 pKa = 2.78AFTYY530 pKa = 10.33FGNTLVTMAMIAYY543 pKa = 9.86ASDD546 pKa = 5.5LSDD549 pKa = 3.87CDD551 pKa = 3.8CAIFSGDD558 pKa = 3.74DD559 pKa = 3.21SLIISKK565 pKa = 9.29VKK567 pKa = 10.21PVLDD571 pKa = 3.36TDD573 pKa = 3.79MFTSLFNMEE582 pKa = 4.84IKK584 pKa = 11.01VMDD587 pKa = 4.23PSVPYY592 pKa = 9.97VCSKK596 pKa = 10.59FLVEE600 pKa = 4.3TEE602 pKa = 3.89MGNLVSVPDD611 pKa = 4.47PLRR614 pKa = 11.84EE615 pKa = 3.79IQRR618 pKa = 11.84LAKK621 pKa = 10.23RR622 pKa = 11.84KK623 pKa = 9.27ILRR626 pKa = 11.84DD627 pKa = 3.46EE628 pKa = 4.15QMLRR632 pKa = 11.84AHH634 pKa = 6.69FVSFCDD640 pKa = 2.86RR641 pKa = 11.84MKK643 pKa = 10.97FINQLDD649 pKa = 3.82EE650 pKa = 5.16KK651 pKa = 10.47MITTLCHH658 pKa = 5.93FVYY661 pKa = 10.47LKK663 pKa = 10.61YY664 pKa = 10.72GKK666 pKa = 9.08EE667 pKa = 3.9KK668 pKa = 9.37PWIFEE673 pKa = 3.91EE674 pKa = 4.57VRR676 pKa = 11.84AALAAFSLYY685 pKa = 10.71SEE687 pKa = 4.15NFLRR691 pKa = 11.84FSDD694 pKa = 4.81CYY696 pKa = 8.1CTEE699 pKa = 4.83GIRR702 pKa = 11.84VYY704 pKa = 11.13QMSDD708 pKa = 3.25PVCKK712 pKa = 10.67FKK714 pKa = 10.62RR715 pKa = 11.84TTEE718 pKa = 3.83EE719 pKa = 4.05RR720 pKa = 11.84KK721 pKa = 8.3TDD723 pKa = 3.54GDD725 pKa = 3.7WFHH728 pKa = 6.22NWKK731 pKa = 9.94NPKK734 pKa = 9.91FPGVLDD740 pKa = 3.48KK741 pKa = 11.06VYY743 pKa = 9.43RR744 pKa = 11.84TIGIYY749 pKa = 10.59SSDD752 pKa = 3.94CSTKK756 pKa = 9.32EE757 pKa = 3.88LPVKK761 pKa = 10.55RR762 pKa = 11.84IGRR765 pKa = 11.84LHH767 pKa = 6.32EE768 pKa = 4.23ALEE771 pKa = 4.32RR772 pKa = 11.84EE773 pKa = 4.41SLKK776 pKa = 10.75LANDD780 pKa = 3.08RR781 pKa = 11.84RR782 pKa = 11.84TTQRR786 pKa = 11.84LKK788 pKa = 11.14KK789 pKa = 10.45KK790 pKa = 10.11VDD792 pKa = 3.73DD793 pKa = 4.02YY794 pKa = 11.18ATGRR798 pKa = 11.84GGLTSVDD805 pKa = 3.18ALLVKK810 pKa = 8.2SHH812 pKa = 7.24CEE814 pKa = 3.65TFKK817 pKa = 11.13PSDD820 pKa = 3.46LRR822 pKa = 4.93

Molecular weight: 93.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P03603|MVP_BMV Movement protein OS=Brome mosaic virus OX=12302 GN=ORF3a PE=1 SV=1
MM1 pKa = 6.91STSGTGKK8 pKa = 7.38MTRR11 pKa = 11.84AQRR14 pKa = 11.84RR15 pKa = 11.84AAARR19 pKa = 11.84RR20 pKa = 11.84NRR22 pKa = 11.84WTARR26 pKa = 11.84VQPVIVEE33 pKa = 4.05PLAAGQGKK41 pKa = 9.95AIKK44 pKa = 10.19AIAGYY49 pKa = 10.07SISKK53 pKa = 9.61WEE55 pKa = 4.16ASSDD59 pKa = 3.85AITAKK64 pKa = 9.48ATNAMSITLPHH75 pKa = 6.47EE76 pKa = 4.38LSSEE80 pKa = 4.06KK81 pKa = 10.79NKK83 pKa = 9.76EE84 pKa = 4.11LKK86 pKa = 10.11VGRR89 pKa = 11.84VLLWLGLLPSVAGRR103 pKa = 11.84IKK105 pKa = 10.67ACVAEE110 pKa = 4.39KK111 pKa = 10.23QAQAEE116 pKa = 4.2AAFQVALAVADD127 pKa = 3.7SSKK130 pKa = 10.93EE131 pKa = 3.86VVAAMYY137 pKa = 9.95TDD139 pKa = 4.01AFRR142 pKa = 11.84GATLGDD148 pKa = 3.96LLNLQIYY155 pKa = 9.3LYY157 pKa = 10.83ASEE160 pKa = 4.94AVPAKK165 pKa = 10.38AVVVHH170 pKa = 6.37LEE172 pKa = 4.18VEE174 pKa = 4.59HH175 pKa = 6.17VRR177 pKa = 11.84PTFDD181 pKa = 5.07DD182 pKa = 4.32FFTPVYY188 pKa = 10.25RR189 pKa = 5.11

Molecular weight: 20.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2275

189

961

568.8

64.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.956 ± 1.434

2.33 ± 0.384

7.604 ± 0.99

6.022 ± 0.216

4.22 ± 0.559

5.451 ± 0.517

2.725 ± 0.468

4.791 ± 0.111

6.418 ± 0.068

8.484 ± 0.134

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.374 ± 0.344

3.121 ± 0.229

3.736 ± 0.415

3.209 ± 0.106

6.11 ± 0.319

8.132 ± 0.826

5.451 ± 0.246

7.297 ± 0.414

1.582 ± 0.199

2.989 ± 0.082
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski