Spiroplasma phage SkV1CR23x

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Plectroviridae; Vespertiliovirus; Spiroplasma virus SkV1CR23x

Average proteome isoelectric point is 8.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8RHJ1|A8RHJ1_9VIRU Uncharacterized protein OS=Spiroplasma phage SkV1CR23x OX=2789014 PE=4 SV=1
MM1 pKa = 7.48SLYY4 pKa = 10.38DD5 pKa = 3.35YY6 pKa = 9.68WVQFVSYY13 pKa = 10.02IIGANAPEE21 pKa = 4.16FLYY24 pKa = 11.05VISFVLFIVLFFGIFFKK41 pKa = 11.0LIQKK45 pKa = 7.64MWSFF49 pKa = 3.91

Molecular weight: 5.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8RHK0|A8RHK0_9VIRU Uncharacterized protein OS=Spiroplasma phage SkV1CR23x OX=2789014 PE=4 SV=1
MM1 pKa = 7.81INLLVEE7 pKa = 4.5NNNSNWDD14 pKa = 3.76KK15 pKa = 10.97IFSFVFDD22 pKa = 3.42IFLFIFDD29 pKa = 4.91VIWNTKK35 pKa = 10.34LPMTNTSIAYY45 pKa = 8.28FLIFFMVIKK54 pKa = 10.38LSIYY58 pKa = 9.85AIHH61 pKa = 6.33GTSTQYY67 pKa = 11.67NNLGSTVNNGVSQIYY82 pKa = 10.29SSTVRR87 pKa = 11.84KK88 pKa = 9.63GINVGKK94 pKa = 10.05NVYY97 pKa = 10.03QNSNKK102 pKa = 9.39QQVKK106 pKa = 10.31RR107 pKa = 11.84EE108 pKa = 4.07IKK110 pKa = 9.77RR111 pKa = 11.84QNIRR115 pKa = 11.84QQAKK119 pKa = 8.93IKK121 pKa = 10.5RR122 pKa = 11.84GVKK125 pKa = 9.69

Molecular weight: 14.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

2253

36

728

173.3

20.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.752 ± 0.221

0.577 ± 0.26

4.616 ± 0.585

4.439 ± 0.424

9.543 ± 0.478

4.172 ± 0.548

0.888 ± 0.207

10.031 ± 1.098

10.608 ± 1.04

9.809 ± 0.667

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.997 ± 0.467

9.055 ± 1.046

1.997 ± 0.179

3.418 ± 0.298

2.708 ± 0.303

5.459 ± 0.516

4.439 ± 0.415

5.326 ± 0.428

2.219 ± 0.322

5.948 ± 0.743
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski