Soft spider associated circular virus 1
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346BP97|A0A346BP97_9CIRC Putative capsid protein OS=Soft spider associated circular virus 1 OX=2293301 PE=4 SV=1
MM1 pKa = 6.28 TTWPASASTADD12 pKa = 3.69 AAPPDD17 pKa = 4.05 GSAPSAEE24 pKa = 4.22 PGEE27 pKa = 4.53 AEE29 pKa = 5.29 GDD31 pKa = 3.49 QTDD34 pKa = 3.42 AKK36 pKa = 10.12 LTFTASVWLPEE47 pKa = 4.2 LQSSHH52 pKa = 7.43 LLVQSSATDD61 pKa = 2.78 ICLRR65 pKa = 11.84 VDD67 pKa = 4.56 DD68 pKa = 5.54 LPFNNTQIGYY78 pKa = 9.27 LYY80 pKa = 10.87 SHH82 pKa = 6.87 LRR84 pKa = 11.84 INKK87 pKa = 8.79 CVVKK91 pKa = 10.34 IYY93 pKa = 10.05 PCGNFAEE100 pKa = 4.41 QPVAGSYY107 pKa = 10.07 IEE109 pKa = 4.23 PEE111 pKa = 4.49 VYY113 pKa = 10.56 SSITYY118 pKa = 10.01 DD119 pKa = 3.58 PQVVDD124 pKa = 3.46 ATYY127 pKa = 10.27 TLTNGPTVKK136 pKa = 10.24 SHH138 pKa = 6.18 GPRR141 pKa = 11.84 SKK143 pKa = 9.01 ITRR146 pKa = 11.84 VVYY149 pKa = 9.44 PKK151 pKa = 10.09 VCYY154 pKa = 9.18 PVWGVNGDD162 pKa = 3.34 ISSTSSSIRR171 pKa = 11.84 NMKK174 pKa = 8.6 KK175 pKa = 10.48 APWMSINSAGITDD188 pKa = 3.76 ACLGKK193 pKa = 10.48 LRR195 pKa = 11.84 IYY197 pKa = 10.14 MGANSGDD204 pKa = 4.2 GITKK208 pKa = 7.96 VWKK211 pKa = 9.1 WNVTKK216 pKa = 10.02 TYY218 pKa = 10.63 YY219 pKa = 10.46 FQLKK223 pKa = 9.5 NVV225 pKa = 3.62
Molecular weight: 24.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.197
IPC2_protein 6.3
IPC_protein 6.504
Toseland 6.44
ProMoST 6.898
Dawson 6.985
Bjellqvist 6.81
Wikipedia 6.956
Rodwell 6.971
Grimsley 6.491
Solomon 7.029
Lehninger 7.044
Nozaki 7.161
DTASelect 7.263
Thurlkill 7.293
EMBOSS 7.337
Sillero 7.38
Patrickios 3.452
IPC_peptide 7.044
IPC2_peptide 6.898
IPC2.peptide.svr19 6.869
Protein with the highest isoelectric point:
>tr|A0A346BP97|A0A346BP97_9CIRC Putative capsid protein OS=Soft spider associated circular virus 1 OX=2293301 PE=4 SV=1
MM1 pKa = 7.32 AQGKK5 pKa = 8.81 RR6 pKa = 11.84 WCFTLNNYY14 pKa = 8.6 TDD16 pKa = 3.82 GDD18 pKa = 3.95 EE19 pKa = 4.06 EE20 pKa = 4.41 RR21 pKa = 11.84 LRR23 pKa = 11.84 GLDD26 pKa = 3.37 VKK28 pKa = 9.0 YY29 pKa = 10.51 LCVGRR34 pKa = 11.84 EE35 pKa = 3.88 VAPTTGTRR43 pKa = 11.84 HH44 pKa = 5.15 LQGFVNFRR52 pKa = 11.84 TCQRR56 pKa = 11.84 FNKK59 pKa = 9.49 ARR61 pKa = 11.84 EE62 pKa = 4.12 LIGTSAHH69 pKa = 6.64 LEE71 pKa = 4.08 LAKK74 pKa = 10.28 GTDD77 pKa = 3.69 VQNKK81 pKa = 8.25 EE82 pKa = 4.39 YY83 pKa = 9.98 CCKK86 pKa = 10.48 SADD89 pKa = 3.62 FFEE92 pKa = 5.12 VCVQGQRR99 pKa = 11.84 TDD101 pKa = 3.55 LAAVAAACAVRR112 pKa = 11.84 GNSLEE117 pKa = 4.14 KK118 pKa = 10.68 VALEE122 pKa = 4.31 HH123 pKa = 5.77 PQEE126 pKa = 4.36 FIKK129 pKa = 9.74 YY130 pKa = 8.0 HH131 pKa = 6.49 RR132 pKa = 11.84 GIQRR136 pKa = 11.84 YY137 pKa = 7.98 YY138 pKa = 10.59 EE139 pKa = 3.79 IANPIQHH146 pKa = 7.2 RR147 pKa = 11.84 DD148 pKa = 3.11 FKK150 pKa = 10.64 TRR152 pKa = 11.84 VHH154 pKa = 6.01 VFVGPPGSGKK164 pKa = 10.22 SRR166 pKa = 11.84 LANACGEE173 pKa = 4.48 VNGEE177 pKa = 3.7 IYY179 pKa = 10.68 YY180 pKa = 10.31 KK181 pKa = 10.54 PRR183 pKa = 11.84 GEE185 pKa = 3.68 WWDD188 pKa = 3.77 GYY190 pKa = 10.64 RR191 pKa = 11.84 DD192 pKa = 3.45 QSTIVVDD199 pKa = 5.96 DD200 pKa = 4.16 FYY202 pKa = 11.95 GWLKK206 pKa = 10.72 YY207 pKa = 10.67 DD208 pKa = 4.31 EE209 pKa = 4.82 LLKK212 pKa = 10.7 ICDD215 pKa = 3.62 RR216 pKa = 11.84 YY217 pKa = 9.79 PYY219 pKa = 9.79 KK220 pKa = 11.1 VPVKK224 pKa = 9.61 GAYY227 pKa = 9.74 VEE229 pKa = 4.65 FTARR233 pKa = 11.84 NIFITSNEE241 pKa = 4.73 PIDD244 pKa = 3.35 NWYY247 pKa = 10.1 KK248 pKa = 10.75 FNGYY252 pKa = 10.07 DD253 pKa = 3.14 PAAIKK258 pKa = 10.51 RR259 pKa = 11.84 RR260 pKa = 11.84 VSVYY264 pKa = 9.6 MFINGLEE271 pKa = 3.89 PVYY274 pKa = 10.91 YY275 pKa = 10.03 IGPDD279 pKa = 3.96 DD280 pKa = 3.79 EE281 pKa = 4.37 WLSLKK286 pKa = 10.51 VKK288 pKa = 10.87 AEE290 pKa = 4.0 FKK292 pKa = 10.28 IACQNVLLDD301 pKa = 4.51 LDD303 pKa = 4.25 NNN305 pKa = 4.12
Molecular weight: 35.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.823
IPC2_protein 7.044
IPC_protein 6.985
Toseland 6.678
ProMoST 7.497
Dawson 7.644
Bjellqvist 7.951
Wikipedia 7.571
Rodwell 7.658
Grimsley 6.722
Solomon 7.702
Lehninger 7.717
Nozaki 8.17
DTASelect 7.805
Thurlkill 7.849
EMBOSS 7.878
Sillero 8.156
Patrickios 3.884
IPC_peptide 7.702
IPC2_peptide 7.278
IPC2.peptide.svr19 7.24
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
530
225
305
265.0
29.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.547 ± 0.27
2.83 ± 0.363
5.849 ± 0.308
5.094 ± 1.185
3.585 ± 1.079
7.17 ± 0.301
1.698 ± 0.218
5.283 ± 0.296
6.226 ± 0.268
6.415 ± 0.381
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.132 ± 0.386
5.283 ± 0.235
5.283 ± 1.092
3.396 ± 0.17
5.094 ± 1.45
6.038 ± 2.5
6.038 ± 1.438
7.925 ± 0.045
2.264 ± 0.24
5.849 ± 0.308
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here