Amino acid dipepetide frequency for Anaerobacillus alkaliphilus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.912AlaAla: 4.912 ± 0.093	0.546AlaCys: 0.546 ± 0.021	2.976AlaAsp: 2.976 ± 0.052	4.254AlaGlu: 4.254 ± 0.065	3.07AlaPhe: 3.07 ± 0.054	4.569AlaGly: 4.569 ± 0.069	1.221AlaHis: 1.221 ± 0.027	5.891AlaIle: 5.891 ± 0.087	4.511AlaLys: 4.511 ± 0.077	6.817AlaLeu: 6.817 ± 0.079	1.813AlaMet: 1.813 ± 0.038	3.068AlaAsn: 3.068 ± 0.048	2.014AlaPro: 2.014 ± 0.05	2.057AlaGln: 2.057 ± 0.044	2.525AlaArg: 2.525 ± 0.045	3.992AlaSer: 3.992 ± 0.06	3.867AlaThr: 3.867 ± 0.066	4.997AlaVal: 4.997 ± 0.08	0.594AlaTrp: 0.594 ± 0.021	2.169AlaTyr: 2.169 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	0.358CysAla: 0.358 ± 0.017	0.104CysCys: 0.104 ± 0.009	0.375CysAsp: 0.375 ± 0.02	0.453CysGlu: 0.453 ± 0.018	0.35CysPhe: 0.35 ± 0.016	0.647CysGly: 0.647 ± 0.024	0.253CysHis: 0.253 ± 0.019	0.554CysIle: 0.554 ± 0.022	0.368CysLys: 0.368 ± 0.017	0.684CysLeu: 0.684 ± 0.022	0.163CysMet: 0.163 ± 0.012	0.317CysAsn: 0.317 ± 0.016	0.344CysPro: 0.344 ± 0.019	0.239CysGln: 0.239 ± 0.014	0.302CysArg: 0.302 ± 0.016	0.51CysSer: 0.51 ± 0.024	0.388CysThr: 0.388 ± 0.018	0.413CysVal: 0.413 ± 0.017	0.075CysTrp: 0.075 ± 0.008	0.26CysTyr: 0.26 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	2.913AspAla: 2.913 ± 0.046	0.381AspCys: 0.381 ± 0.018	2.379AspAsp: 2.379 ± 0.048	4.193AspGlu: 4.193 ± 0.059	2.421AspPhe: 2.421 ± 0.046	3.341AspGly: 3.341 ± 0.059	1.243AspHis: 1.243 ± 0.036	4.036AspIle: 4.036 ± 0.054	2.825AspLys: 2.825 ± 0.055	5.066AspLeu: 5.066 ± 0.06	1.227AspMet: 1.227 ± 0.03	1.808AspAsn: 1.808 ± 0.041	2.022AspPro: 2.022 ± 0.045	2.179AspGln: 2.179 ± 0.041	2.252AspArg: 2.252 ± 0.046	2.136AspSer: 2.136 ± 0.035	2.354AspThr: 2.354 ± 0.041	3.888AspVal: 3.888 ± 0.058	0.601AspTrp: 0.601 ± 0.02	2.128AspTyr: 2.128 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	5.37GluAla: 5.37 ± 0.08	0.361GluCys: 0.361 ± 0.016	3.797GluAsp: 3.797 ± 0.059	7.104GluGlu: 7.104 ± 0.106	2.874GluPhe: 2.874 ± 0.046	4.387GluGly: 4.387 ± 0.068	1.469GluHis: 1.469 ± 0.035	5.917GluIle: 5.917 ± 0.075	6.391GluLys: 6.391 ± 0.081	7.543GluLeu: 7.543 ± 0.098	2.13GluMet: 2.13 ± 0.04	3.693GluAsn: 3.693 ± 0.056	1.914GluPro: 1.914 ± 0.04	3.288GluGln: 3.288 ± 0.055	3.494GluArg: 3.494 ± 0.057	2.881GluSer: 2.881 ± 0.045	4.132GluThr: 4.132 ± 0.064	5.705GluVal: 5.705 ± 0.074	0.797GluTrp: 0.797 ± 0.025	2.293GluTyr: 2.293 ± 0.046	0.0GluXaa: 0.0 ± 0.0
Phe	3.032PheAla: 3.032 ± 0.051	0.363PheCys: 0.363 ± 0.016	2.403PheAsp: 2.403 ± 0.038	3.006PheGlu: 3.006 ± 0.051	2.567PhePhe: 2.567 ± 0.057	3.367PheGly: 3.367 ± 0.055	1.129PheHis: 1.129 ± 0.025	4.106PheIle: 4.106 ± 0.068	2.379PheLys: 2.379 ± 0.045	5.058PheLeu: 5.058 ± 0.084	1.154PheMet: 1.154 ± 0.027	1.868PheAsn: 1.868 ± 0.037	1.751PhePro: 1.751 ± 0.039	1.861PheGln: 1.861 ± 0.037	1.687PheArg: 1.687 ± 0.033	3.416PheSer: 3.416 ± 0.053	2.799PheThr: 2.799 ± 0.047	3.416PheVal: 3.416 ± 0.05	0.498PheTrp: 0.498 ± 0.021	1.859PheTyr: 1.859 ± 0.039	0.0PheXaa: 0.0 ± 0.0
Gly	4.512GlyAla: 4.512 ± 0.071	0.619GlyCys: 0.619 ± 0.024	3.144GlyAsp: 3.144 ± 0.052	4.492GlyGlu: 4.492 ± 0.063	3.54GlyPhe: 3.54 ± 0.061	4.555GlyGly: 4.555 ± 0.076	1.399GlyHis: 1.399 ± 0.037	5.737GlyIle: 5.737 ± 0.064	4.717GlyLys: 4.717 ± 0.063	6.499GlyLeu: 6.499 ± 0.082	1.903GlyMet: 1.903 ± 0.041	2.978GlyAsn: 2.978 ± 0.054	1.74GlyPro: 1.74 ± 0.038	2.239GlyGln: 2.239 ± 0.043	2.628GlyArg: 2.628 ± 0.048	3.883GlySer: 3.883 ± 0.062	4.024GlyThr: 4.024 ± 0.056	5.129GlyVal: 5.129 ± 0.057	0.759GlyTrp: 0.759 ± 0.027	2.792GlyTyr: 2.792 ± 0.051	0.0GlyXaa: 0.0 ± 0.0
His	1.193HisAla: 1.193 ± 0.032	0.204HisCys: 0.204 ± 0.012	1.011HisAsp: 1.011 ± 0.03	1.436HisGlu: 1.436 ± 0.034	1.115HisPhe: 1.115 ± 0.033	1.447HisGly: 1.447 ± 0.036	0.657HisHis: 0.657 ± 0.026	1.492HisIle: 1.492 ± 0.039	1.026HisLys: 1.026 ± 0.028	2.2HisLeu: 2.2 ± 0.045	0.507HisMet: 0.507 ± 0.022	0.852HisAsn: 0.852 ± 0.029	1.09HisPro: 1.09 ± 0.027	0.888HisGln: 0.888 ± 0.027	0.888HisArg: 0.888 ± 0.027	1.265HisSer: 1.265 ± 0.034	1.019HisThr: 1.019 ± 0.031	1.456HisVal: 1.456 ± 0.034	0.283HisTrp: 0.283 ± 0.016	0.922HisTyr: 0.922 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	5.74IleAla: 5.74 ± 0.076	0.615IleCys: 0.615 ± 0.024	4.398IleAsp: 4.398 ± 0.063	6.168IleGlu: 6.168 ± 0.083	3.656IlePhe: 3.656 ± 0.059	5.981IleGly: 5.981 ± 0.074	1.659IleHis: 1.659 ± 0.035	6.399IleIle: 6.399 ± 0.082	4.434IleLys: 4.434 ± 0.059	7.337IleLeu: 7.337 ± 0.086	1.693IleMet: 1.693 ± 0.04	3.679IleAsn: 3.679 ± 0.061	3.254IlePro: 3.254 ± 0.049	2.763IleGln: 2.763 ± 0.048	3.029IleArg: 3.029 ± 0.052	5.276IleSer: 5.276 ± 0.077	4.736IleThr: 4.736 ± 0.059	5.887IleVal: 5.887 ± 0.066	0.641IleTrp: 0.641 ± 0.023	2.584IleTyr: 2.584 ± 0.046	0.0IleXaa: 0.0 ± 0.0
Lys	4.235LysAla: 4.235 ± 0.055	0.33LysCys: 0.33 ± 0.017	3.447LysAsp: 3.447 ± 0.054	6.3LysGlu: 6.3 ± 0.083	1.998LysPhe: 1.998 ± 0.041	4.243LysGly: 4.243 ± 0.064	1.351LysHis: 1.351 ± 0.035	4.722LysIle: 4.722 ± 0.057	5.391LysLys: 5.391 ± 0.076	6.097LysLeu: 6.097 ± 0.067	1.986LysMet: 1.986 ± 0.042	3.196LysAsn: 3.196 ± 0.049	2.072LysPro: 2.072 ± 0.04	3.046LysGln: 3.046 ± 0.053	3.003LysArg: 3.003 ± 0.048	3.292LysSer: 3.292 ± 0.052	3.482LysThr: 3.482 ± 0.05	4.88LysVal: 4.88 ± 0.076	0.74LysTrp: 0.74 ± 0.026	2.111LysTyr: 2.111 ± 0.04	0.0LysXaa: 0.0 ± 0.0
Leu	7.392LeuAla: 7.392 ± 0.104	0.651LeuCys: 0.651 ± 0.022	4.772LeuAsp: 4.772 ± 0.065	7.161LeuGlu: 7.161 ± 0.08	5.107LeuPhe: 5.107 ± 0.077	6.557LeuGly: 6.557 ± 0.093	2.077LeuHis: 2.077 ± 0.046	7.27LeuIle: 7.27 ± 0.087	6.364LeuLys: 6.364 ± 0.077	10.696LeuLeu: 10.696 ± 0.128	2.429LeuMet: 2.429 ± 0.041	4.345LeuAsn: 4.345 ± 0.06	3.916LeuPro: 3.916 ± 0.052	3.719LeuGln: 3.719 ± 0.056	3.809LeuArg: 3.809 ± 0.052	6.546LeuSer: 6.546 ± 0.08	5.951LeuThr: 5.951 ± 0.072	7.104LeuVal: 7.104 ± 0.084	0.861LeuTrp: 0.861 ± 0.028	3.218LeuTyr: 3.218 ± 0.058	0.0LeuXaa: 0.0 ± 0.0
Met	1.875MetAla: 1.875 ± 0.035	0.14MetCys: 0.14 ± 0.011	1.253MetAsp: 1.253 ± 0.029	1.829MetGlu: 1.829 ± 0.041	1.174MetPhe: 1.174 ± 0.027	1.611MetGly: 1.611 ± 0.036	0.386MetHis: 0.386 ± 0.016	2.096MetIle: 2.096 ± 0.038	2.287MetLys: 2.287 ± 0.041	2.383MetLeu: 2.383 ± 0.045	0.82MetMet: 0.82 ± 0.029	1.465MetAsn: 1.465 ± 0.033	0.915MetPro: 0.915 ± 0.033	0.762MetGln: 0.762 ± 0.025	1.063MetArg: 1.063 ± 0.028	1.577MetSer: 1.577 ± 0.04	1.566MetThr: 1.566 ± 0.035	1.818MetVal: 1.818 ± 0.037	0.189MetTrp: 0.189 ± 0.013	0.789MetTyr: 0.789 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	2.579AsnAla: 2.579 ± 0.046	0.343AsnCys: 0.343 ± 0.017	2.41AsnAsp: 2.41 ± 0.043	3.858AsnGlu: 3.858 ± 0.051	1.96AsnPhe: 1.96 ± 0.038	3.28AsnGly: 3.28 ± 0.058	1.102AsnHis: 1.102 ± 0.033	3.485AsnIle: 3.485 ± 0.049	2.833AsnLys: 2.833 ± 0.049	4.115AsnLeu: 4.115 ± 0.057	1.167AsnMet: 1.167 ± 0.034	2.271AsnAsn: 2.271 ± 0.051	2.126AsnPro: 2.126 ± 0.044	2.044AsnGln: 2.044 ± 0.038	1.984AsnArg: 1.984 ± 0.036	2.638AsnSer: 2.638 ± 0.047	2.131AsnThr: 2.131 ± 0.039	3.239AsnVal: 3.239 ± 0.051	0.567AsnTrp: 0.567 ± 0.019	1.829AsnTyr: 1.829 ± 0.039	0.0AsnXaa: 0.0 ± 0.0
Pro	2.059ProAla: 2.059 ± 0.042	0.203ProCys: 0.203 ± 0.012	1.727ProAsp: 1.727 ± 0.041	2.788ProGlu: 2.788 ± 0.049	2.061ProPhe: 2.061 ± 0.041	2.258ProGly: 2.258 ± 0.048	0.755ProHis: 0.755 ± 0.025	2.955ProIle: 2.955 ± 0.046	2.11ProLys: 2.11 ± 0.041	3.476ProLeu: 3.476 ± 0.053	0.883ProMet: 0.883 ± 0.028	1.848ProAsn: 1.848 ± 0.04	1.017ProPro: 1.017 ± 0.035	1.094ProGln: 1.094 ± 0.036	1.16ProArg: 1.16 ± 0.028	2.23ProSer: 2.23 ± 0.04	2.261ProThr: 2.261 ± 0.041	2.697ProVal: 2.697 ± 0.041	0.4ProTrp: 0.4 ± 0.017	1.414ProTyr: 1.414 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	2.54GlnAla: 2.54 ± 0.055	0.222GlnCys: 0.222 ± 0.013	1.575GlnAsp: 1.575 ± 0.031	2.819GlnGlu: 2.819 ± 0.05	1.777GlnPhe: 1.777 ± 0.038	2.25GlnGly: 2.25 ± 0.04	0.74GlnHis: 0.74 ± 0.028	2.71GlnIle: 2.71 ± 0.046	2.542GlnLys: 2.542 ± 0.051	4.373GlnLeu: 4.373 ± 0.06	1.143GlnMet: 1.143 ± 0.027	1.528GlnAsn: 1.528 ± 0.036	1.275GlnPro: 1.275 ± 0.036	1.895GlnGln: 1.895 ± 0.057	1.586GlnArg: 1.586 ± 0.038	2.066GlnSer: 2.066 ± 0.041	2.053GlnThr: 2.053 ± 0.037	2.634GlnVal: 2.634 ± 0.055	0.424GlnTrp: 0.424 ± 0.019	1.401GlnTyr: 1.401 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	2.402ArgAla: 2.402 ± 0.041	0.263ArgCys: 0.263 ± 0.015	2.083ArgAsp: 2.083 ± 0.049	3.27ArgGlu: 3.27 ± 0.056	1.907ArgPhe: 1.907 ± 0.036	2.475ArgGly: 2.475 ± 0.044	0.738ArgHis: 0.738 ± 0.023	2.997ArgIle: 2.997 ± 0.06	3.049ArgLys: 3.049 ± 0.048	4.051ArgLeu: 4.051 ± 0.058	1.154ArgMet: 1.154 ± 0.028	2.05ArgAsn: 2.05 ± 0.043	1.31ArgPro: 1.31 ± 0.031	1.494ArgGln: 1.494 ± 0.037	1.77ArgArg: 1.77 ± 0.044	2.338ArgSer: 2.338 ± 0.037	2.114ArgThr: 2.114 ± 0.042	2.813ArgVal: 2.813 ± 0.046	0.443ArgTrp: 0.443 ± 0.018	1.586ArgTyr: 1.586 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	3.254SerAla: 3.254 ± 0.053	0.435SerCys: 0.435 ± 0.019	2.723SerAsp: 2.723 ± 0.046	3.995SerGlu: 3.995 ± 0.055	3.379SerPhe: 3.379 ± 0.05	4.084SerGly: 4.084 ± 0.064	1.137SerHis: 1.137 ± 0.031	5.22SerIle: 5.22 ± 0.066	3.731SerLys: 3.731 ± 0.052	6.326SerLeu: 6.326 ± 0.068	1.567SerMet: 1.567 ± 0.034	2.766SerAsn: 2.766 ± 0.053	2.021SerPro: 2.021 ± 0.043	2.017SerGln: 2.017 ± 0.039	2.165SerArg: 2.165 ± 0.043	3.947SerSer: 3.947 ± 0.065	3.221SerThr: 3.221 ± 0.055	4.04SerVal: 4.04 ± 0.045	0.663SerTrp: 0.663 ± 0.023	2.379SerTyr: 2.379 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	3.682ThrAla: 3.682 ± 0.066	0.391ThrCys: 0.391 ± 0.02	2.61ThrAsp: 2.61 ± 0.044	3.704ThrGlu: 3.704 ± 0.051	2.898ThrPhe: 2.898 ± 0.055	4.043ThrGly: 4.043 ± 0.061	1.111ThrHis: 1.111 ± 0.026	5.075ThrIle: 5.075 ± 0.064	3.621ThrLys: 3.621 ± 0.051	5.511ThrLeu: 5.511 ± 0.062	1.356ThrMet: 1.356 ± 0.036	2.814ThrAsn: 2.814 ± 0.047	2.249ThrPro: 2.249 ± 0.044	1.489ThrGln: 1.489 ± 0.037	2.066ThrArg: 2.066 ± 0.04	3.467ThrSer: 3.467 ± 0.054	3.293ThrThr: 3.293 ± 0.048	4.403ThrVal: 4.403 ± 0.058	0.565ThrTrp: 0.565 ± 0.021	2.033ThrTyr: 2.033 ± 0.041	0.0ThrXaa: 0.0 ± 0.0
Val	5.299ValAla: 5.299 ± 0.077	0.563ValCys: 0.563 ± 0.021	3.84ValAsp: 3.84 ± 0.066	5.313ValGlu: 5.313 ± 0.068	3.319ValPhe: 3.319 ± 0.052	5.034ValGly: 5.034 ± 0.076	1.357ValHis: 1.357 ± 0.032	6.015ValIle: 6.015 ± 0.07	4.667ValLys: 4.667 ± 0.063	6.786ValLeu: 6.786 ± 0.068	1.782ValMet: 1.782 ± 0.04	3.372ValAsn: 3.372 ± 0.051	2.692ValPro: 2.692 ± 0.042	2.388ValGln: 2.388 ± 0.038	2.822ValArg: 2.822 ± 0.049	4.771ValSer: 4.771 ± 0.057	4.681ValThr: 4.681 ± 0.073	5.715ValVal: 5.715 ± 0.077	0.613ValTrp: 0.613 ± 0.024	2.308ValTyr: 2.308 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	0.616TrpAla: 0.616 ± 0.023	0.087TrpCys: 0.087 ± 0.007	0.573TrpAsp: 0.573 ± 0.024	0.71TrpGlu: 0.71 ± 0.027	0.518TrpPhe: 0.518 ± 0.021	0.687TrpGly: 0.687 ± 0.026	0.207TrpHis: 0.207 ± 0.013	0.765TrpIle: 0.765 ± 0.029	0.657TrpLys: 0.657 ± 0.023	1.166TrpLeu: 1.166 ± 0.034	0.314TrpMet: 0.314 ± 0.015	0.538TrpAsn: 0.538 ± 0.021	0.271TrpPro: 0.271 ± 0.014	0.391TrpGln: 0.391 ± 0.018	0.408TrpArg: 0.408 ± 0.018	0.611TrpSer: 0.611 ± 0.022	0.51TrpThr: 0.51 ± 0.017	0.724TrpVal: 0.724 ± 0.024	0.162TrpTrp: 0.162 ± 0.011	0.355TrpTyr: 0.355 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.902TyrAla: 1.902 ± 0.037	0.336TyrCys: 0.336 ± 0.016	1.898TyrAsp: 1.898 ± 0.036	2.684TyrGlu: 2.684 ± 0.049	2.027TyrPhe: 2.027 ± 0.039	2.474TyrGly: 2.474 ± 0.044	0.959TyrHis: 0.959 ± 0.03	2.486TyrIle: 2.486 ± 0.044	2.02TyrLys: 2.02 ± 0.04	3.761TyrLeu: 3.761 ± 0.057	0.806TyrMet: 0.806 ± 0.024	1.505TyrAsn: 1.505 ± 0.031	1.403TyrPro: 1.403 ± 0.035	1.703TyrGln: 1.703 ± 0.038	1.667TyrArg: 1.667 ± 0.041	2.228TyrSer: 2.228 ± 0.046	1.799TyrThr: 1.799 ± 0.044	2.378TyrVal: 2.378 ± 0.037	0.395TyrTrp: 0.395 ± 0.019	1.594TyrTyr: 1.594 ± 0.039	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4643 proteins (1361674 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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