Alteromonas sp. KUL49

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Alteromonas; unclassified Alteromonas

Average proteome isoelectric point is 5.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3846 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q8UHW2|A0A4Q8UHW2_9ALTE Sulfate adenylyltransferase subunit 1 OS=Alteromonas sp. KUL49 OX=2480798 GN=cysN PE=3 SV=1
MM1 pKa = 6.87MHH3 pKa = 7.82LLRR6 pKa = 11.84LVAMCATTLAFGTNAAVIDD25 pKa = 4.37NEE27 pKa = 4.33DD28 pKa = 3.52SLAGSFIDD36 pKa = 4.1TNTNLVWMDD45 pKa = 3.47FGINNNYY52 pKa = 9.0TYY54 pKa = 11.31YY55 pKa = 10.8EE56 pKa = 4.43VVSKK60 pKa = 10.78LADD63 pKa = 3.71GGEE66 pKa = 4.26FEE68 pKa = 4.72GWRR71 pKa = 11.84LPDD74 pKa = 3.44YY75 pKa = 11.48DD76 pKa = 4.52EE77 pKa = 5.81VYY79 pKa = 11.46DD80 pKa = 3.49MWFGVINASNSDD92 pKa = 3.13IDD94 pKa = 3.67RR95 pKa = 11.84VRR97 pKa = 11.84EE98 pKa = 3.88DD99 pKa = 3.92FYY101 pKa = 11.75GPGTDD106 pKa = 5.24LYY108 pKa = 11.48LDD110 pKa = 4.38ANSDD114 pKa = 3.86GADD117 pKa = 3.31DD118 pKa = 4.64SVWDD122 pKa = 3.7NAFDD126 pKa = 3.71VMGYY130 pKa = 10.47NSLGTGFTGFDD141 pKa = 3.39YY142 pKa = 10.84TKK144 pKa = 10.88AWGLFAGEE152 pKa = 4.24DD153 pKa = 3.34AMYY156 pKa = 10.6SIVFDD161 pKa = 4.54DD162 pKa = 5.04FLDD165 pKa = 4.32LEE167 pKa = 4.65VDD169 pKa = 3.55GEE171 pKa = 4.32TRR173 pKa = 11.84EE174 pKa = 4.91DD175 pKa = 3.88SIAFNNGWNQEE186 pKa = 3.95YY187 pKa = 10.24LADD190 pKa = 5.93DD191 pKa = 3.75INTNLSTLLVRR202 pKa = 11.84VTEE205 pKa = 4.32TVNVSEE211 pKa = 5.0PNTIALFTVVLCAFVTVRR229 pKa = 11.84RR230 pKa = 11.84RR231 pKa = 11.84EE232 pKa = 3.83EE233 pKa = 4.24SATHH237 pKa = 6.13

Molecular weight: 26.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q8UMF8|A0A4Q8UMF8_9ALTE Uncharacterized protein OS=Alteromonas sp. KUL49 OX=2480798 GN=EYS00_00530 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.14RR12 pKa = 11.84KK13 pKa = 9.13RR14 pKa = 11.84SHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 9.16VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.29GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3846

0

3846

1317231

37

2390

342.5

37.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.634 ± 0.041

0.936 ± 0.014

5.919 ± 0.032

6.216 ± 0.033

4.329 ± 0.024

6.821 ± 0.032

2.214 ± 0.019

6.086 ± 0.026

4.662 ± 0.035

9.945 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.434 ± 0.021

4.526 ± 0.031

3.908 ± 0.023

4.213 ± 0.027

4.463 ± 0.024

7.162 ± 0.037

5.781 ± 0.033

7.297 ± 0.03

1.258 ± 0.015

3.196 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski