Streptococcus phage Javan88

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6BIW5|A0A4D6BIW5_9CAUD Uncharacterized protein OS=Streptococcus phage Javan88 OX=2548312 GN=Javan88_0023 PE=4 SV=1
MM1 pKa = 7.54TKK3 pKa = 10.59NNYY6 pKa = 8.52YY7 pKa = 10.63VSAKK11 pKa = 10.05KK12 pKa = 10.57DD13 pKa = 3.57NLDD16 pKa = 3.31LGMVIEE22 pKa = 4.83AEE24 pKa = 4.33NPYY27 pKa = 9.05MAAVKK32 pKa = 9.78MSSLLWDD39 pKa = 3.58EE40 pKa = 4.7FSFDD44 pKa = 3.61EE45 pKa = 4.78VAVTDD50 pKa = 3.84VEE52 pKa = 4.39LVGMEE57 pKa = 4.22VQNDD61 pKa = 3.31

Molecular weight: 6.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6BF30|A0A4D6BF30_9CAUD Uncharacterized protein OS=Streptococcus phage Javan88 OX=2548312 GN=Javan88_0055 PE=4 SV=1
MM1 pKa = 7.68AKK3 pKa = 10.44GFVTRR8 pKa = 11.84AHH10 pKa = 6.44VDD12 pKa = 3.13LGGVTKK18 pKa = 10.37KK19 pKa = 10.62VSPEE23 pKa = 3.48AVRR26 pKa = 11.84KK27 pKa = 9.57GRR29 pKa = 11.84QAMANQILLDD39 pKa = 3.52TDD41 pKa = 3.93AYY43 pKa = 11.12VPLRR47 pKa = 11.84DD48 pKa = 3.38GSLRR52 pKa = 11.84ASASIGLQGEE62 pKa = 4.46TVNWNTVYY70 pKa = 10.94ARR72 pKa = 11.84AQFYY76 pKa = 7.61GTNGIVTFRR85 pKa = 11.84KK86 pKa = 8.62YY87 pKa = 6.24TTQGTGKK94 pKa = 9.44NWYY97 pKa = 9.04VKK99 pKa = 10.61AKK101 pKa = 10.48NKK103 pKa = 10.26HH104 pKa = 5.11LDD106 pKa = 3.11NWKK109 pKa = 9.06DD110 pKa = 3.51TAVKK114 pKa = 10.81AMGLKK119 pKa = 10.15

Molecular weight: 13.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

12079

39

1265

204.7

22.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.807 ± 0.974

0.43 ± 0.081

6.275 ± 0.39

6.391 ± 0.613

4.131 ± 0.19

6.962 ± 0.618

1.391 ± 0.144

6.78 ± 0.239

8.08 ± 0.531

7.956 ± 0.341

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.608 ± 0.185

5.812 ± 0.427

2.74 ± 0.202

4.363 ± 0.3

3.527 ± 0.297

6.573 ± 0.595

6.764 ± 0.366

6.408 ± 0.385

1.06 ± 0.137

3.941 ± 0.383
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski