Amino acid dipepetide frequency for Lactococcus phage MV16

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.868AlaAla: 1.868 ± 0.931
0.439AlaCys: 0.439 ± 0.274
2.966AlaAsp: 2.966 ± 0.585
4.505AlaGlu: 4.505 ± 0.82
3.955AlaPhe: 3.955 ± 0.951
3.955AlaGly: 3.955 ± 0.536
0.549AlaHis: 0.549 ± 0.205
4.724AlaIle: 4.724 ± 0.884
6.372AlaLys: 6.372 ± 0.804
5.603AlaLeu: 5.603 ± 0.856
2.087AlaMet: 2.087 ± 0.574
5.823AlaAsn: 5.823 ± 1.055
0.769AlaPro: 0.769 ± 0.31
2.637AlaGln: 2.637 ± 0.573
2.417AlaArg: 2.417 ± 0.521
3.406AlaSer: 3.406 ± 0.909
4.505AlaThr: 4.505 ± 1.079
4.834AlaVal: 4.834 ± 1.221
1.758AlaTrp: 1.758 ± 0.776
1.978AlaTyr: 1.978 ± 0.407
0.0AlaXaa: 0.0 ± 0.0
Cys
0.33CysAla: 0.33 ± 0.194
0.11CysCys: 0.11 ± 0.105
0.11CysAsp: 0.11 ± 0.112
0.439CysGlu: 0.439 ± 0.206
0.0CysPhe: 0.0 ± 0.0
0.549CysGly: 0.549 ± 0.268
0.11CysHis: 0.11 ± 0.101
0.549CysIle: 0.549 ± 0.26
0.33CysLys: 0.33 ± 0.217
0.659CysLeu: 0.659 ± 0.326
0.11CysMet: 0.11 ± 0.112
0.33CysAsn: 0.33 ± 0.225
0.11CysPro: 0.11 ± 0.111
0.33CysGln: 0.33 ± 0.198
0.33CysArg: 0.33 ± 0.187
0.22CysSer: 0.22 ± 0.15
0.33CysThr: 0.33 ± 0.169
0.33CysVal: 0.33 ± 0.196
0.0CysTrp: 0.0 ± 0.0
0.33CysTyr: 0.33 ± 0.185
0.0CysXaa: 0.0 ± 0.0
Asp
2.417AspAla: 2.417 ± 0.517
0.33AspCys: 0.33 ± 0.197
2.747AspAsp: 2.747 ± 0.589
4.175AspGlu: 4.175 ± 0.789
3.406AspPhe: 3.406 ± 0.511
4.175AspGly: 4.175 ± 0.605
0.769AspHis: 0.769 ± 0.309
3.735AspIle: 3.735 ± 0.744
5.603AspLys: 5.603 ± 0.751
6.482AspLeu: 6.482 ± 0.786
1.209AspMet: 1.209 ± 0.306
4.505AspAsn: 4.505 ± 0.601
1.758AspPro: 1.758 ± 0.446
0.439AspGln: 0.439 ± 0.271
1.758AspArg: 1.758 ± 0.519
2.197AspSer: 2.197 ± 0.537
4.285AspThr: 4.285 ± 0.833
2.307AspVal: 2.307 ± 0.658
1.428AspTrp: 1.428 ± 0.393
2.637AspTyr: 2.637 ± 0.46
0.0AspXaa: 0.0 ± 0.0
Glu
3.955GluAla: 3.955 ± 0.693
0.33GluCys: 0.33 ± 0.194
3.076GluAsp: 3.076 ± 0.439
5.823GluGlu: 5.823 ± 1.244
3.406GluPhe: 3.406 ± 0.49
2.087GluGly: 2.087 ± 0.438
0.989GluHis: 0.989 ± 0.342
6.262GluIle: 6.262 ± 0.928
5.493GluLys: 5.493 ± 1.245
9.998GluLeu: 9.998 ± 1.627
3.076GluMet: 3.076 ± 0.652
4.614GluAsn: 4.614 ± 0.754
1.428GluPro: 1.428 ± 0.387
3.955GluGln: 3.955 ± 0.803
3.076GluArg: 3.076 ± 0.715
3.516GluSer: 3.516 ± 0.623
4.285GluThr: 4.285 ± 0.843
5.603GluVal: 5.603 ± 0.78
1.318GluTrp: 1.318 ± 0.396
3.076GluTyr: 3.076 ± 0.663
0.0GluXaa: 0.0 ± 0.0
Phe
3.516PheAla: 3.516 ± 0.809
0.33PheCys: 0.33 ± 0.216
3.626PheAsp: 3.626 ± 0.679
3.186PheGlu: 3.186 ± 0.77
2.307PhePhe: 2.307 ± 0.652
2.747PheGly: 2.747 ± 0.597
0.22PheHis: 0.22 ± 0.21
3.516PheIle: 3.516 ± 0.686
3.845PheLys: 3.845 ± 0.594
2.527PheLeu: 2.527 ± 0.504
0.879PheMet: 0.879 ± 0.294
3.076PheAsn: 3.076 ± 0.916
0.769PhePro: 0.769 ± 0.263
1.099PheGln: 1.099 ± 0.425
1.538PheArg: 1.538 ± 0.325
3.406PheSer: 3.406 ± 0.709
3.296PheThr: 3.296 ± 0.481
2.197PheVal: 2.197 ± 0.382
0.33PheTrp: 0.33 ± 0.175
1.648PheTyr: 1.648 ± 0.431
0.0PheXaa: 0.0 ± 0.0
Gly
3.406GlyAla: 3.406 ± 0.787
0.11GlyCys: 0.11 ± 0.096
3.076GlyAsp: 3.076 ± 0.674
3.516GlyGlu: 3.516 ± 0.625
2.966GlyPhe: 2.966 ± 0.673
3.735GlyGly: 3.735 ± 0.766
0.439GlyHis: 0.439 ± 0.207
3.955GlyIle: 3.955 ± 1.33
5.274GlyLys: 5.274 ± 0.676
6.262GlyLeu: 6.262 ± 1.103
1.099GlyMet: 1.099 ± 0.366
3.076GlyAsn: 3.076 ± 0.588
0.33GlyPro: 0.33 ± 0.173
1.648GlyGln: 1.648 ± 0.425
2.197GlyArg: 2.197 ± 0.454
6.043GlySer: 6.043 ± 1.029
2.966GlyThr: 2.966 ± 0.577
5.164GlyVal: 5.164 ± 1.22
1.099GlyTrp: 1.099 ± 0.337
3.406GlyTyr: 3.406 ± 0.671
0.0GlyXaa: 0.0 ± 0.0
His
0.769HisAla: 0.769 ± 0.253
0.33HisCys: 0.33 ± 0.181
0.769HisAsp: 0.769 ± 0.308
1.099HisGlu: 1.099 ± 0.448
0.769HisPhe: 0.769 ± 0.39
1.209HisGly: 1.209 ± 0.424
0.0HisHis: 0.0 ± 0.0
0.659HisIle: 0.659 ± 0.263
0.879HisLys: 0.879 ± 0.315
1.099HisLeu: 1.099 ± 0.414
0.0HisMet: 0.0 ± 0.0
1.538HisAsn: 1.538 ± 0.384
0.22HisPro: 0.22 ± 0.147
0.33HisGln: 0.33 ± 0.155
0.22HisArg: 0.22 ± 0.16
0.33HisSer: 0.33 ± 0.233
0.769HisThr: 0.769 ± 0.263
0.659HisVal: 0.659 ± 0.285
0.0HisTrp: 0.0 ± 0.0
0.769HisTyr: 0.769 ± 0.295
0.0HisXaa: 0.0 ± 0.0
Ile
4.944IleAla: 4.944 ± 0.739
0.33IleCys: 0.33 ± 0.18
4.614IleAsp: 4.614 ± 0.732
6.152IleGlu: 6.152 ± 0.997
2.637IlePhe: 2.637 ± 0.6
3.296IleGly: 3.296 ± 0.71
0.989IleHis: 0.989 ± 0.322
4.834IleIle: 4.834 ± 0.716
6.702IleLys: 6.702 ± 1.021
5.933IleLeu: 5.933 ± 0.892
1.318IleMet: 1.318 ± 0.356
5.933IleAsn: 5.933 ± 0.615
2.197IlePro: 2.197 ± 0.508
1.868IleGln: 1.868 ± 0.431
2.087IleArg: 2.087 ± 0.456
4.724IleSer: 4.724 ± 0.871
4.175IleThr: 4.175 ± 0.655
3.845IleVal: 3.845 ± 0.695
1.538IleTrp: 1.538 ± 0.464
2.197IleTyr: 2.197 ± 0.405
0.0IleXaa: 0.0 ± 0.0
Lys
6.922LysAla: 6.922 ± 0.879
0.439LysCys: 0.439 ± 0.206
5.054LysAsp: 5.054 ± 0.695
8.46LysGlu: 8.46 ± 1.387
2.197LysPhe: 2.197 ± 0.551
5.603LysGly: 5.603 ± 0.797
1.868LysHis: 1.868 ± 0.548
5.713LysIle: 5.713 ± 0.666
8.46LysLys: 8.46 ± 1.091
7.581LysLeu: 7.581 ± 0.738
2.747LysMet: 2.747 ± 0.482
4.614LysAsn: 4.614 ± 0.706
2.307LysPro: 2.307 ± 0.635
4.285LysGln: 4.285 ± 0.685
3.296LysArg: 3.296 ± 0.802
5.054LysSer: 5.054 ± 0.733
5.054LysThr: 5.054 ± 0.782
6.152LysVal: 6.152 ± 0.812
1.318LysTrp: 1.318 ± 0.405
3.186LysTyr: 3.186 ± 0.569
0.0LysXaa: 0.0 ± 0.0
Leu
5.603LeuAla: 5.603 ± 0.761
0.33LeuCys: 0.33 ± 0.189
4.614LeuAsp: 4.614 ± 0.639
6.152LeuGlu: 6.152 ± 0.881
3.296LeuPhe: 3.296 ± 0.692
4.724LeuGly: 4.724 ± 0.646
0.989LeuHis: 0.989 ± 0.361
7.031LeuIle: 7.031 ± 0.985
8.46LeuLys: 8.46 ± 0.8
7.141LeuLeu: 7.141 ± 1.169
0.989LeuMet: 0.989 ± 0.326
5.713LeuAsn: 5.713 ± 0.834
3.186LeuPro: 3.186 ± 0.57
3.735LeuGln: 3.735 ± 0.708
3.296LeuArg: 3.296 ± 0.622
4.614LeuSer: 4.614 ± 0.904
5.933LeuThr: 5.933 ± 0.765
5.713LeuVal: 5.713 ± 0.724
1.978LeuTrp: 1.978 ± 0.415
3.955LeuTyr: 3.955 ± 0.663
0.0LeuXaa: 0.0 ± 0.0
Met
2.307MetAla: 2.307 ± 0.49
0.0MetCys: 0.0 ± 0.0
0.989MetAsp: 0.989 ± 0.422
1.648MetGlu: 1.648 ± 0.567
0.769MetPhe: 0.769 ± 0.272
0.659MetGly: 0.659 ± 0.258
0.439MetHis: 0.439 ± 0.204
2.307MetIle: 2.307 ± 0.466
2.857MetLys: 2.857 ± 0.634
1.318MetLeu: 1.318 ± 0.399
0.549MetMet: 0.549 ± 0.267
1.538MetAsn: 1.538 ± 0.397
0.439MetPro: 0.439 ± 0.252
1.538MetGln: 1.538 ± 0.364
0.659MetArg: 0.659 ± 0.239
1.538MetSer: 1.538 ± 0.348
2.197MetThr: 2.197 ± 0.604
1.538MetVal: 1.538 ± 0.357
0.0MetTrp: 0.0 ± 0.0
0.989MetTyr: 0.989 ± 0.326
0.0MetXaa: 0.0 ± 0.0
Asn
5.054AsnAla: 5.054 ± 0.934
0.22AsnCys: 0.22 ± 0.157
4.505AsnAsp: 4.505 ± 0.608
4.614AsnGlu: 4.614 ± 0.771
1.978AsnPhe: 1.978 ± 0.615
5.493AsnGly: 5.493 ± 0.717
0.879AsnHis: 0.879 ± 0.306
4.065AsnIle: 4.065 ± 0.708
6.592AsnLys: 6.592 ± 1.16
5.274AsnLeu: 5.274 ± 1.004
1.868AsnMet: 1.868 ± 0.406
3.626AsnAsn: 3.626 ± 0.795
2.087AsnPro: 2.087 ± 0.472
1.868AsnGln: 1.868 ± 0.472
2.307AsnArg: 2.307 ± 0.432
5.823AsnSer: 5.823 ± 0.758
4.505AsnThr: 4.505 ± 0.946
3.955AsnVal: 3.955 ± 0.602
1.209AsnTrp: 1.209 ± 0.33
2.637AsnTyr: 2.637 ± 0.741
0.0AsnXaa: 0.0 ± 0.0
Pro
1.318ProAla: 1.318 ± 0.43
0.22ProCys: 0.22 ± 0.149
1.758ProAsp: 1.758 ± 0.468
1.978ProGlu: 1.978 ± 0.547
1.318ProPhe: 1.318 ± 0.387
0.22ProGly: 0.22 ± 0.147
0.0ProHis: 0.0 ± 0.0
2.087ProIle: 2.087 ± 0.548
1.978ProLys: 1.978 ± 0.533
1.978ProLeu: 1.978 ± 0.498
0.659ProMet: 0.659 ± 0.23
2.747ProAsn: 2.747 ± 0.846
0.879ProPro: 0.879 ± 0.35
0.769ProGln: 0.769 ± 0.28
0.549ProArg: 0.549 ± 0.21
0.879ProSer: 0.879 ± 0.308
1.868ProThr: 1.868 ± 0.419
1.538ProVal: 1.538 ± 0.437
0.33ProTrp: 0.33 ± 0.177
0.879ProTyr: 0.879 ± 0.375
0.0ProXaa: 0.0 ± 0.0
Gln
3.186GlnAla: 3.186 ± 0.756
0.22GlnCys: 0.22 ± 0.17
2.527GlnAsp: 2.527 ± 0.505
2.197GlnGlu: 2.197 ± 0.493
1.318GlnPhe: 1.318 ± 0.422
2.857GlnGly: 2.857 ± 0.553
0.439GlnHis: 0.439 ± 0.254
1.428GlnIle: 1.428 ± 0.362
3.186GlnLys: 3.186 ± 0.611
3.296GlnLeu: 3.296 ± 0.704
0.879GlnMet: 0.879 ± 0.289
1.648GlnAsn: 1.648 ± 0.429
1.428GlnPro: 1.428 ± 0.365
1.428GlnGln: 1.428 ± 0.445
1.978GlnArg: 1.978 ± 0.445
1.758GlnSer: 1.758 ± 0.348
1.978GlnThr: 1.978 ± 0.468
1.648GlnVal: 1.648 ± 0.41
0.769GlnTrp: 0.769 ± 0.249
1.209GlnTyr: 1.209 ± 0.374
0.0GlnXaa: 0.0 ± 0.0
Arg
2.307ArgAla: 2.307 ± 0.691
0.22ArgCys: 0.22 ± 0.145
1.978ArgAsp: 1.978 ± 0.536
2.527ArgGlu: 2.527 ± 0.589
0.989ArgPhe: 0.989 ± 0.3
2.417ArgGly: 2.417 ± 0.475
0.769ArgHis: 0.769 ± 0.275
1.758ArgIle: 1.758 ± 0.444
4.175ArgLys: 4.175 ± 0.9
3.296ArgLeu: 3.296 ± 0.686
0.549ArgMet: 0.549 ± 0.26
2.747ArgAsn: 2.747 ± 0.652
0.549ArgPro: 0.549 ± 0.243
1.428ArgGln: 1.428 ± 0.359
1.428ArgArg: 1.428 ± 0.441
1.648ArgSer: 1.648 ± 0.36
2.307ArgThr: 2.307 ± 0.441
2.087ArgVal: 2.087 ± 0.505
0.22ArgTrp: 0.22 ± 0.157
1.758ArgTyr: 1.758 ± 0.462
0.0ArgXaa: 0.0 ± 0.0
Ser
5.823SerAla: 5.823 ± 1.798
0.549SerCys: 0.549 ± 0.3
4.065SerAsp: 4.065 ± 0.732
4.065SerGlu: 4.065 ± 0.649
3.186SerPhe: 3.186 ± 0.642
5.493SerGly: 5.493 ± 1.659
0.549SerHis: 0.549 ± 0.271
4.285SerIle: 4.285 ± 0.805
5.164SerLys: 5.164 ± 0.826
4.505SerLeu: 4.505 ± 0.728
2.197SerMet: 2.197 ± 0.366
4.285SerAsn: 4.285 ± 0.899
1.099SerPro: 1.099 ± 0.403
2.307SerGln: 2.307 ± 0.527
1.978SerArg: 1.978 ± 0.429
4.614SerSer: 4.614 ± 0.962
2.857SerThr: 2.857 ± 0.598
4.065SerVal: 4.065 ± 0.671
0.659SerTrp: 0.659 ± 0.273
1.428SerTyr: 1.428 ± 0.385
0.0SerXaa: 0.0 ± 0.0
Thr
4.505ThrAla: 4.505 ± 0.636
0.22ThrCys: 0.22 ± 0.14
3.296ThrAsp: 3.296 ± 0.649
5.713ThrGlu: 5.713 ± 0.713
2.197ThrPhe: 2.197 ± 0.463
4.065ThrGly: 4.065 ± 0.66
0.22ThrHis: 0.22 ± 0.168
4.395ThrIle: 4.395 ± 0.719
4.505ThrLys: 4.505 ± 0.639
5.603ThrLeu: 5.603 ± 0.774
1.099ThrMet: 1.099 ± 0.34
4.614ThrAsn: 4.614 ± 0.98
1.758ThrPro: 1.758 ± 0.382
2.527ThrGln: 2.527 ± 0.582
1.648ThrArg: 1.648 ± 0.456
4.724ThrSer: 4.724 ± 0.661
4.614ThrThr: 4.614 ± 0.715
4.944ThrVal: 4.944 ± 0.827
0.879ThrTrp: 0.879 ± 0.281
1.868ThrTyr: 1.868 ± 0.393
0.0ThrXaa: 0.0 ± 0.0
Val
4.505ValAla: 4.505 ± 0.577
0.22ValCys: 0.22 ± 0.149
3.845ValAsp: 3.845 ± 0.632
4.724ValGlu: 4.724 ± 0.754
3.626ValPhe: 3.626 ± 0.743
3.406ValGly: 3.406 ± 0.582
0.769ValHis: 0.769 ± 0.264
4.614ValIle: 4.614 ± 0.729
6.043ValLys: 6.043 ± 0.815
4.065ValLeu: 4.065 ± 0.701
1.868ValMet: 1.868 ± 0.422
2.966ValAsn: 2.966 ± 0.596
1.318ValPro: 1.318 ± 0.396
1.538ValGln: 1.538 ± 0.349
2.637ValArg: 2.637 ± 0.567
5.383ValSer: 5.383 ± 1.286
4.505ValThr: 4.505 ± 0.919
4.505ValVal: 4.505 ± 0.918
0.33ValTrp: 0.33 ± 0.188
2.747ValTyr: 2.747 ± 0.495
0.0ValXaa: 0.0 ± 0.0
Trp
0.879TrpAla: 0.879 ± 0.291
0.33TrpCys: 0.33 ± 0.203
0.659TrpAsp: 0.659 ± 0.301
0.879TrpGlu: 0.879 ± 0.297
1.428TrpPhe: 1.428 ± 0.407
0.879TrpGly: 0.879 ± 0.268
0.22TrpHis: 0.22 ± 0.16
0.989TrpIle: 0.989 ± 0.321
0.989TrpLys: 0.989 ± 0.296
1.209TrpLeu: 1.209 ± 0.359
0.33TrpMet: 0.33 ± 0.178
1.648TrpAsn: 1.648 ± 0.449
0.0TrpPro: 0.0 ± 0.0
0.769TrpGln: 0.769 ± 0.296
0.439TrpArg: 0.439 ± 0.247
1.538TrpSer: 1.538 ± 0.404
0.769TrpThr: 0.769 ± 0.268
0.439TrpVal: 0.439 ± 0.312
0.11TrpTrp: 0.11 ± 0.096
0.989TrpTyr: 0.989 ± 0.318
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.538TyrAla: 1.538 ± 0.444
0.33TyrCys: 0.33 ± 0.223
2.087TyrAsp: 2.087 ± 0.584
3.516TyrGlu: 3.516 ± 0.813
2.307TyrPhe: 2.307 ± 0.437
2.197TyrGly: 2.197 ± 0.492
1.209TyrHis: 1.209 ± 0.328
3.406TyrIle: 3.406 ± 0.752
3.516TyrLys: 3.516 ± 0.765
3.626TyrLeu: 3.626 ± 0.82
0.659TyrMet: 0.659 ± 0.263
3.516TyrAsn: 3.516 ± 0.564
1.318TyrPro: 1.318 ± 0.446
0.879TyrGln: 0.879 ± 0.281
1.318TyrArg: 1.318 ± 0.371
1.758TyrSer: 1.758 ± 0.358
2.197TyrThr: 2.197 ± 0.54
2.087TyrVal: 2.087 ± 0.477
0.22TyrTrp: 0.22 ± 0.136
2.527TyrTyr: 2.527 ± 0.659
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (9103 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski