Ralstonia phage Firinga

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Firingavirus; Ralstonia virus Firinga

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G5B9X1|A0A7G5B9X1_9CAUD Uncharacterized protein OS=Ralstonia phage Firinga OX=2759725 GN=18C_00026 PE=4 SV=1
MM1 pKa = 7.5SLGITASVVGGIGAIAGGVISANGAQSAADD31 pKa = 3.73TQANATQSGIAQQNYY46 pKa = 7.22QFNTLRR52 pKa = 11.84GLLQPYY58 pKa = 9.72VDD60 pKa = 4.66AGTGALSGYY69 pKa = 9.96NSAVGQYY76 pKa = 10.8GSTLGQLNNLTGANGQAAQQSAISGLTSSPLYY108 pKa = 9.04ATAMDD113 pKa = 5.28LGQQSILANASATGALRR130 pKa = 11.84NGNTIASLGYY140 pKa = 10.33LPQQVLSNVMQTQIGNLGTSLSGTQSLVNQYY171 pKa = 11.28GNLLNLGEE179 pKa = 4.27NAASGTGQAAMNTGSNITNLLGQQGAAQAGGTIGTSNAINSAINGVSSAFGQYY232 pKa = 11.44ANGSNGNYY240 pKa = 9.8SFSAPAYY247 pKa = 9.14LNAGGISTNGSTTGLFNTPLFNN269 pKa = 4.8

Molecular weight: 26.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G5B9Z4|A0A7G5B9Z4_9CAUD Uncharacterized protein OS=Ralstonia phage Firinga OX=2759725 GN=18C_00049 PE=4 SV=1
MM1 pKa = 7.44SRR3 pKa = 11.84THH5 pKa = 6.56LVGNFLRR12 pKa = 11.84QFGVVVQSTGRR23 pKa = 11.84VGFDD27 pKa = 3.84DD28 pKa = 3.81VLSANRR34 pKa = 11.84TGPCKK39 pKa = 10.48VFPSPMGISCRR50 pKa = 11.84RR51 pKa = 11.84AATSFANHH59 pKa = 5.63VCHH62 pKa = 6.62VLDD65 pKa = 3.81VSAWIQMARR74 pKa = 11.84IYY76 pKa = 11.14AKK78 pKa = 10.49AYY80 pKa = 8.81VAA82 pKa = 4.86

Molecular weight: 8.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

12072

56

1280

236.7

25.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.798 ± 0.617

0.903 ± 0.152

5.393 ± 0.275

4.978 ± 0.502

2.866 ± 0.242

8.35 ± 0.457

1.483 ± 0.168

4.954 ± 0.265

3.786 ± 0.307

7.488 ± 0.288

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.469 ± 0.156

4.548 ± 0.369

4.614 ± 0.261

5.716 ± 0.369

5.674 ± 0.378

6.66 ± 0.405

6.917 ± 0.391

6.403 ± 0.32

1.359 ± 0.217

2.642 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski