halophilic archaeon J07HX64

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; unclassified Halobacteriales

Average proteome isoelectric point is 5.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3025 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U1QTE3|U1QTE3_9EURY Double-stranded beta-helix domain protein OS=halophilic archaeon J07HX64 OX=1085028 GN=J07HX64_02293 PE=4 SV=1
MM1 pKa = 7.44EE2 pKa = 4.86LTWHH6 pKa = 6.17GHH8 pKa = 3.98STWRR12 pKa = 11.84VEE14 pKa = 4.26LGEE17 pKa = 4.17TSMLIDD23 pKa = 4.16PFFGNPKK30 pKa = 9.25TGLSPEE36 pKa = 4.33SVEE39 pKa = 4.22TPDD42 pKa = 4.57YY43 pKa = 11.38VLLTHH48 pKa = 6.42GHH50 pKa = 6.98ADD52 pKa = 3.74HH53 pKa = 7.07IGDD56 pKa = 3.81AAAFSDD62 pKa = 3.92ATLVAVPEE70 pKa = 4.19LAAFAEE76 pKa = 4.62DD77 pKa = 3.89EE78 pKa = 4.76LGFDD82 pKa = 4.48DD83 pKa = 5.82AVAGMGMNMGGTVACGDD100 pKa = 3.92AFVTMHH106 pKa = 7.22RR107 pKa = 11.84ADD109 pKa = 3.63HH110 pKa = 6.23TNGANTGYY118 pKa = 10.4DD119 pKa = 3.9LEE121 pKa = 6.4LGMPVGYY128 pKa = 10.15VISDD132 pKa = 3.94TAPTEE137 pKa = 4.43LDD139 pKa = 3.9DD140 pKa = 5.41ADD142 pKa = 3.8STTFYY147 pKa = 10.67HH148 pKa = 7.25AGDD151 pKa = 3.73TALMTEE157 pKa = 3.86MRR159 pKa = 11.84EE160 pKa = 4.21VIGAYY165 pKa = 10.19LDD167 pKa = 3.86PDD169 pKa = 3.94AAALPAGDD177 pKa = 4.83HH178 pKa = 5.59FTMGPRR184 pKa = 11.84QAAIAAGWLGVDD196 pKa = 3.83YY197 pKa = 10.28ALPMHH202 pKa = 7.05YY203 pKa = 8.78DD204 pKa = 3.75TFPPIEE210 pKa = 4.9IDD212 pKa = 3.06TATFEE217 pKa = 5.02DD218 pKa = 4.7EE219 pKa = 4.8LGAHH223 pKa = 6.6APDD226 pKa = 3.83TEE228 pKa = 4.17AVVLDD233 pKa = 3.75GDD235 pKa = 4.11EE236 pKa = 4.41SFEE239 pKa = 4.27LL240 pKa = 4.16

Molecular weight: 25.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U1QYS5|U1QYS5_9EURY Uncharacterized protein OS=halophilic archaeon J07HX64 OX=1085028 GN=J07HX64_02918 PE=4 SV=1
MM1 pKa = 7.59CSIASTRR8 pKa = 11.84EE9 pKa = 3.12RR10 pKa = 11.84SFRR13 pKa = 11.84AKK15 pKa = 10.14RR16 pKa = 11.84GKK18 pKa = 10.25LLEE21 pKa = 4.99LGTLYY26 pKa = 11.09LDD28 pKa = 3.44VPYY31 pKa = 11.07GFVSDD36 pKa = 4.55IEE38 pKa = 5.13DD39 pKa = 4.0GEE41 pKa = 4.23QLITSSVGNHH51 pKa = 7.03DD52 pKa = 4.3LLQPASRR59 pKa = 11.84ARR61 pKa = 11.84SGSRR65 pKa = 11.84TAGRR69 pKa = 11.84RR70 pKa = 11.84SPPTTASSPSKK81 pKa = 9.9TPVEE85 pKa = 3.99AAGQRR90 pKa = 11.84TQPTRR95 pKa = 11.84CSNWRR100 pKa = 11.84RR101 pKa = 11.84TSAAGG106 pKa = 3.2

Molecular weight: 11.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3025

0

3025

767293

23

3137

253.7

27.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.974 ± 0.062

0.804 ± 0.018

7.865 ± 0.053

8.343 ± 0.068

3.05 ± 0.029

9.032 ± 0.057

1.993 ± 0.026

3.513 ± 0.034

1.397 ± 0.025

8.752 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.622 ± 0.021

2.344 ± 0.03

4.777 ± 0.031

2.874 ± 0.029

7.165 ± 0.057

5.799 ± 0.042

7.062 ± 0.045

9.095 ± 0.059

1.005 ± 0.015

2.384 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski