Candidatus Tokpelaia sp. JSC189

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiales incertae sedis; Candidatus Tokpelaia; unclassified Candidatus Tokpelaia

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1271 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A367ZVR7|A0A367ZVR7_9RHIZ Zinc import ATP-binding protein ZnuC OS=Candidatus Tokpelaia sp. JSC189 OX=2079010 GN=znuC PE=3 SV=1
MM1 pKa = 7.33NVGNMLATQGPILKK15 pKa = 10.45LSDD18 pKa = 3.52VVGKK22 pKa = 10.23IDD24 pKa = 3.67GDD26 pKa = 4.0GAVKK30 pKa = 9.98IVRR33 pKa = 11.84YY34 pKa = 9.6GVVSDD39 pKa = 4.24CEE41 pKa = 3.74EE42 pKa = 5.5DD43 pKa = 3.19ITEE46 pKa = 5.0EE47 pKa = 3.8IASCYY52 pKa = 9.94VEE54 pKa = 3.96EE55 pKa = 5.74HH56 pKa = 6.36YY57 pKa = 11.38DD58 pKa = 3.93EE59 pKa = 5.95IDD61 pKa = 3.67EE62 pKa = 4.61EE63 pKa = 4.91SLVAPFIEE71 pKa = 5.29HH72 pKa = 6.12SDD74 pKa = 3.46AYY76 pKa = 10.13EE77 pKa = 4.01DD78 pKa = 4.54HH79 pKa = 7.33LDD81 pKa = 3.72ALEE84 pKa = 4.64AEE86 pKa = 4.4ALEE89 pKa = 4.15DD90 pKa = 3.86SIYY93 pKa = 11.08GSYY96 pKa = 10.67EE97 pKa = 3.58DD98 pKa = 3.79QNRR101 pKa = 11.84LRR103 pKa = 11.84LSDD106 pKa = 3.65VLL108 pKa = 4.22

Molecular weight: 12.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A368A0R3|A0A368A0R3_9RHIZ Uncharacterized protein OS=Candidatus Tokpelaia sp. JSC189 OX=2079010 GN=JSC189_000757 PE=4 SV=1
MM1 pKa = 7.17TEE3 pKa = 3.51IRR5 pKa = 11.84EE6 pKa = 4.21MQVVLCCRR14 pKa = 11.84WRR16 pKa = 11.84HH17 pKa = 3.84VMRR20 pKa = 11.84FIFFNKK26 pKa = 9.53RR27 pKa = 11.84NSLVLAKK34 pKa = 10.19IATHH38 pKa = 6.71LSHH41 pKa = 6.8MGLIRR46 pKa = 11.84NIILRR51 pKa = 11.84MRR53 pKa = 11.84GMLIKK58 pKa = 10.74GSLPEE63 pKa = 3.95VKK65 pKa = 9.79IRR67 pKa = 11.84LLKK70 pKa = 10.6RR71 pKa = 11.84IFSVFMPVLAIFCLLMGAFYY91 pKa = 9.57WVRR94 pKa = 11.84LIGVFSGPLWRR105 pKa = 11.84FDD107 pKa = 3.52LMPWNWRR114 pKa = 11.84ILCSSLAVLYY124 pKa = 9.33PVATSGVWMGSRR136 pKa = 11.84WGIILWLSAAAIEE149 pKa = 4.58TVCMTLYY156 pKa = 11.02SNYY159 pKa = 9.73FSWNLWIPLLHH170 pKa = 6.56VIFLAFYY177 pKa = 10.06GFFSMILFFNQTKK190 pKa = 9.63RR191 pKa = 11.84VQAIVEE197 pKa = 4.31YY198 pKa = 10.89

Molecular weight: 23.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1271

0

1271

371509

29

2863

292.3

32.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.076 ± 0.067

1.067 ± 0.025

5.351 ± 0.048

5.97 ± 0.064

4.24 ± 0.053

7.177 ± 0.063

2.261 ± 0.032

7.962 ± 0.053

5.173 ± 0.049

9.74 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.752 ± 0.033

3.876 ± 0.048

4.052 ± 0.037

3.482 ± 0.041

6.271 ± 0.057

6.035 ± 0.056

5.074 ± 0.045

6.633 ± 0.064

1.085 ± 0.026

2.723 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski