Helicobacter ganmani
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1783 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3D8IHX9|A0A3D8IHX9_9HELI Virulence protein OS=Helicobacter ganmani OX=60246 GN=CQA43_00065 PE=4 SV=1
MM1 pKa = 7.28 SVKK4 pKa = 9.26 ITDD7 pKa = 3.02 ICIACGACIDD17 pKa = 4.08 EE18 pKa = 5.06 CPVEE22 pKa = 5.36 AIVDD26 pKa = 3.96 DD27 pKa = 5.05 DD28 pKa = 5.94 DD29 pKa = 4.73 NPNDD33 pKa = 3.97 DD34 pKa = 2.98 GCYY37 pKa = 9.03 FVHH40 pKa = 6.82 SEE42 pKa = 4.19 KK43 pKa = 10.76 CVEE46 pKa = 4.29 CVGHH50 pKa = 6.61 NDD52 pKa = 3.28 EE53 pKa = 4.91 PACASACPTEE63 pKa = 4.96 GCIVWDD69 pKa = 3.99 AVVASQPHH77 pKa = 6.8 RR78 pKa = 11.84 DD79 pKa = 4.58 DD80 pKa = 4.03 ISDD83 pKa = 3.96 DD84 pKa = 3.27 KK85 pKa = 11.32 RR86 pKa = 11.84 SAHH89 pKa = 5.57 TPVVEE94 pKa = 4.11
Molecular weight: 10.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.867
IPC2_protein 3.935
IPC_protein 3.91
Toseland 3.694
ProMoST 4.05
Dawson 3.91
Bjellqvist 4.113
Wikipedia 3.859
Rodwell 3.745
Grimsley 3.605
Solomon 3.897
Lehninger 3.859
Nozaki 4.037
DTASelect 4.279
Thurlkill 3.757
EMBOSS 3.872
Sillero 4.037
Patrickios 0.299
IPC_peptide 3.897
IPC2_peptide 4.012
IPC2.peptide.svr19 3.96
Protein with the highest isoelectric point:
>tr|A0A3D8I9G8|A0A3D8I9G8_9HELI tRNA/tmRNA (uracil-C(5))-methyltransferase OS=Helicobacter ganmani OX=60246 GN=trmA PE=3 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.04 QPHH8 pKa = 4.86 NTPRR12 pKa = 11.84 KK13 pKa = 7.31 RR14 pKa = 11.84 THH16 pKa = 5.85 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MQTKK25 pKa = 9.97 NGRR28 pKa = 11.84 KK29 pKa = 9.25 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.65 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LAII44 pKa = 4.0
Molecular weight: 5.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1783
0
1783
541776
21
2883
303.9
34.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.059 ± 0.055
1.304 ± 0.027
4.473 ± 0.043
7.16 ± 0.063
5.395 ± 0.052
6.032 ± 0.06
1.799 ± 0.025
8.154 ± 0.051
7.984 ± 0.063
10.962 ± 0.076
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.215 ± 0.026
5.404 ± 0.053
3.246 ± 0.033
4.186 ± 0.047
3.646 ± 0.032
6.668 ± 0.042
4.462 ± 0.041
5.444 ± 0.053
0.743 ± 0.017
3.664 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here