Musa balbisiana (Banana)
Average proteome isoelectric point is 6.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 32103 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4S8IT51|A0A4S8IT51_MUSBA Uncharacterized protein ycf33 OS=Musa balbisiana OX=52838 GN=C4D60_Mb06t25590 PE=3 SV=1
MM1 pKa = 7.72 LLPNYY6 pKa = 9.47 CLAGSSDD13 pKa = 4.05 SDD15 pKa = 3.86 LAIADD20 pKa = 3.97 SEE22 pKa = 4.74 HH23 pKa = 6.81 VVLACLDD30 pKa = 3.52 LQEE33 pKa = 4.73 IVEE36 pKa = 4.6 SVDD39 pKa = 3.37 VACNYY44 pKa = 10.36 SPSQACQQQPVFDD57 pKa = 4.07 VLSEE61 pKa = 4.2 PEE63 pKa = 3.68 IEE65 pKa = 4.73 AYY67 pKa = 10.01 SAEE70 pKa = 4.22 PADD73 pKa = 3.9 EE74 pKa = 4.46 EE75 pKa = 4.59 EE76 pKa = 4.32 NDD78 pKa = 3.3 VAYY81 pKa = 9.73 MVEE84 pKa = 4.29 PEE86 pKa = 4.0 DD87 pKa = 5.18 AIAAVMRR94 pKa = 11.84 ASPAGSAGQNLNGHH108 pKa = 6.47 VIAEE112 pKa = 4.2 EE113 pKa = 3.97 ANPAEE118 pKa = 4.36 EE119 pKa = 4.11 AFVWVALVAVGLALGYY135 pKa = 9.4 EE136 pKa = 4.24 AAVGEE141 pKa = 4.56 GTVDD145 pKa = 3.66 DD146 pKa = 5.09 DD147 pKa = 4.08 VAAEE151 pKa = 3.94 AVEE154 pKa = 4.39 VQTGLAIEE162 pKa = 4.36 QDD164 pKa = 3.7 DD165 pKa = 4.56 EE166 pKa = 4.6 PVVEE170 pKa = 4.99 HH171 pKa = 6.49 VDD173 pKa = 3.51 EE174 pKa = 4.65 AAVGEE179 pKa = 4.48 GTVDD183 pKa = 3.66 DD184 pKa = 5.09 DD185 pKa = 4.16 VAAEE189 pKa = 4.01 AVDD192 pKa = 3.58 IEE194 pKa = 4.88 VPGPAAVVASEE205 pKa = 4.31 DD206 pKa = 3.46 KK207 pKa = 10.72 TVGFDD212 pKa = 3.45 VVGVADD218 pKa = 4.42 QIAGLLFEE226 pKa = 5.72 LGFVDD231 pKa = 6.0 ALACKK236 pKa = 9.82 AAEE239 pKa = 4.07 QVYY242 pKa = 9.25 EE243 pKa = 4.09 AVVSAFAQDD252 pKa = 3.64 GHH254 pKa = 5.15 WVEE257 pKa = 4.71 IGAGAKK263 pKa = 9.18 VAAAVAVEE271 pKa = 4.34 LPVIVAVAVAVAGAGLEE288 pKa = 4.09 AGPGLEE294 pKa = 5.05 AEE296 pKa = 4.71 AEE298 pKa = 4.15 LGDD301 pKa = 4.25 EE302 pKa = 4.87 VEE304 pKa = 4.27 TAHH307 pKa = 6.11 MKK309 pKa = 10.45 KK310 pKa = 10.09 SAAVLEE316 pKa = 4.4 LLAEE320 pKa = 4.41 GQLASEE326 pKa = 4.68 IGHH329 pKa = 4.83 TVGRR333 pKa = 11.84 VAAEE337 pKa = 4.07 IEE339 pKa = 4.18 HH340 pKa = 6.31 SVDD343 pKa = 2.65 IAADD347 pKa = 3.44 KK348 pKa = 10.67 GHH350 pKa = 6.09 SHH352 pKa = 6.02 GTSDD356 pKa = 3.48 STAGIAIVVAAKK368 pKa = 10.36
Molecular weight: 37.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.726
IPC2_protein 3.694
IPC_protein 3.681
Toseland 3.49
ProMoST 3.821
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.541
Rodwell 3.516
Grimsley 3.401
Solomon 3.643
Lehninger 3.605
Nozaki 3.757
DTASelect 3.923
Thurlkill 3.528
EMBOSS 3.554
Sillero 3.795
Patrickios 1.24
IPC_peptide 3.643
IPC2_peptide 3.783
IPC2.peptide.svr19 3.724
Protein with the highest isoelectric point:
>tr|A0A4S8K0Q9|A0A4S8K0Q9_MUSBA Dof-type domain-containing protein OS=Musa balbisiana OX=52838 GN=C4D60_Mb08t01930 PE=4 SV=1
MM1 pKa = 7.33 LVRR4 pKa = 11.84 RR5 pKa = 11.84 QRR7 pKa = 11.84 HH8 pKa = 3.52 VVRR11 pKa = 11.84 RR12 pKa = 11.84 GKK14 pKa = 10.29 RR15 pKa = 11.84 GMRR18 pKa = 11.84 RR19 pKa = 11.84 VLVVEE24 pKa = 4.41 RR25 pKa = 11.84 RR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 LHH30 pKa = 6.32 GIRR33 pKa = 11.84 WHH35 pKa = 5.97 GLGVRR40 pKa = 11.84 WGKK43 pKa = 9.67 RR44 pKa = 11.84 GKK46 pKa = 10.42 RR47 pKa = 11.84 GVVVLKK53 pKa = 10.58 VALRR57 pKa = 11.84 VNPLLLLLRR66 pKa = 11.84 LMLRR70 pKa = 11.84 GNRR73 pKa = 11.84 RR74 pKa = 11.84 RR75 pKa = 11.84 GRR77 pKa = 11.84 RR78 pKa = 11.84 RR79 pKa = 11.84 RR80 pKa = 11.84 WIVVWMVVRR89 pKa = 11.84 RR90 pKa = 11.84 RR91 pKa = 11.84 RR92 pKa = 11.84 RR93 pKa = 11.84 RR94 pKa = 11.84 RR95 pKa = 11.84 RR96 pKa = 11.84 GMGSVAAADD105 pKa = 3.53 AVDD108 pKa = 4.17 RR109 pKa = 11.84 DD110 pKa = 3.92 LHH112 pKa = 6.37 HH113 pKa = 7.27 LLLRR117 pKa = 4.38
Molecular weight: 14.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.468
IPC2_protein 11.023
IPC_protein 12.632
Toseland 12.793
ProMoST 13.29
Dawson 12.793
Bjellqvist 12.793
Wikipedia 13.276
Rodwell 12.34
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.793
DTASelect 12.793
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.062
IPC_peptide 13.29
IPC2_peptide 12.281
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
32103
0
32103
12394127
31
5263
386.1
42.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.924 ± 0.017
1.908 ± 0.007
5.232 ± 0.009
6.3 ± 0.02
3.917 ± 0.011
6.958 ± 0.015
2.496 ± 0.006
4.749 ± 0.011
5.286 ± 0.016
9.715 ± 0.018
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.398 ± 0.006
3.687 ± 0.009
5.434 ± 0.017
3.493 ± 0.01
6.187 ± 0.014
9.103 ± 0.019
4.77 ± 0.008
6.573 ± 0.012
1.299 ± 0.005
2.572 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here