Salmonella phage SW5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Seongnamvirus; unclassified Seongnamvirus

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 38 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C0CGX3|A0A5C0CGX3_9CAUD Uncharacterized protein OS=Salmonella phage SW5 OX=2592222 PE=4 SV=1
MM1 pKa = 6.96STEE4 pKa = 3.98PLYY7 pKa = 10.41IDD9 pKa = 5.6LLITDD14 pKa = 4.8GDD16 pKa = 3.99FTLDD20 pKa = 3.05SGNEE24 pKa = 3.54PRR26 pKa = 11.84RR27 pKa = 11.84CDD29 pKa = 3.41NRR31 pKa = 11.84DD32 pKa = 3.71SITQDD37 pKa = 2.89IIHH40 pKa = 7.07SILEE44 pKa = 4.12SGITTRR50 pKa = 11.84LIGEE54 pKa = 4.9RR55 pKa = 11.84SPTMRR60 pKa = 11.84GDD62 pKa = 3.48VLTQLSLLVEE72 pKa = 4.19SDD74 pKa = 3.17EE75 pKa = 4.43RR76 pKa = 11.84LVPGTIVITEE86 pKa = 4.07EE87 pKa = 4.01TLSRR91 pKa = 11.84LYY93 pKa = 9.43VTAEE97 pKa = 3.99SYY99 pKa = 11.55DD100 pKa = 3.69FGPVGTEE107 pKa = 3.67VNYY110 pKa = 10.73DD111 pKa = 3.16

Molecular weight: 12.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C0CHD3|A0A5C0CHD3_9CAUD Terminase ATPase subunit OS=Salmonella phage SW5 OX=2592222 PE=4 SV=1
MM1 pKa = 6.36VTKK4 pKa = 10.46LQISCAAPVGVCGHH18 pKa = 7.19AAAEE22 pKa = 3.97LRR24 pKa = 11.84RR25 pKa = 11.84FSRR28 pKa = 11.84GVKK31 pKa = 9.21NYY33 pKa = 10.46SRR35 pKa = 11.84IKK37 pKa = 9.22PNFYY41 pKa = 10.16LVIRR45 pKa = 11.84IGRR48 pKa = 11.84RR49 pKa = 11.84WRR51 pKa = 11.84LLSKK55 pKa = 10.87NGGKK59 pKa = 9.1VWSLMTHH66 pKa = 5.69EE67 pKa = 5.04KK68 pKa = 11.11YY69 pKa = 10.62NVEE72 pKa = 4.15SKK74 pKa = 10.74KK75 pKa = 10.95

Molecular weight: 8.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

38

0

38

9497

54

878

249.9

27.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.877 ± 0.556

0.916 ± 0.149

5.633 ± 0.286

5.97 ± 0.33

3.685 ± 0.238

7.518 ± 0.338

1.706 ± 0.175

5.265 ± 0.265

5.349 ± 0.313

8.466 ± 0.398

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.643 ± 0.203

4.328 ± 0.206

4.886 ± 0.283

3.749 ± 0.28

6.118 ± 0.434

5.739 ± 0.234

5.907 ± 0.294

6.57 ± 0.425

1.874 ± 0.195

2.801 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski