Red clover necrotic mosaic virus (RCNMV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Dianthovirus

Average proteome isoelectric point is 7.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P22956-2|RDRP-2_RCNMV Isoform of P22956 Isoform protein p27 of RNA-directed RNA polymerase OS=Red clover necrotic mosaic virus OX=12267 GN=ORF1 PE=1 SV=2
MM1 pKa = 7.52GFINLSLFDD10 pKa = 3.87VDD12 pKa = 5.98KK13 pKa = 11.65LMVWVSKK20 pKa = 9.71FNPGKK25 pKa = 10.11ILSAICNLGIDD36 pKa = 3.47CWNRR40 pKa = 11.84FRR42 pKa = 11.84KK43 pKa = 8.86WFFGLNFDD51 pKa = 3.42AHH53 pKa = 5.56MWAVDD58 pKa = 3.32AFIPLMPHH66 pKa = 4.81YY67 pKa = 8.64TEE69 pKa = 3.78QMEE72 pKa = 4.26RR73 pKa = 11.84VVDD76 pKa = 4.17DD77 pKa = 4.66FCSEE81 pKa = 4.14TPEE84 pKa = 4.87SKK86 pKa = 11.11LEE88 pKa = 4.29DD89 pKa = 4.15CLEE92 pKa = 4.65LDD94 pKa = 3.49TSVNEE99 pKa = 4.22FFDD102 pKa = 3.83EE103 pKa = 4.11EE104 pKa = 5.0VYY106 pKa = 10.98KK107 pKa = 10.62KK108 pKa = 10.67DD109 pKa = 3.34EE110 pKa = 4.42EE111 pKa = 4.77GVMKK115 pKa = 10.25LQRR118 pKa = 11.84SAARR122 pKa = 11.84KK123 pKa = 7.86HH124 pKa = 5.69IKK126 pKa = 9.67RR127 pKa = 11.84VRR129 pKa = 11.84PGMMQAAIKK138 pKa = 10.36AVEE141 pKa = 3.71TRR143 pKa = 11.84IRR145 pKa = 11.84NRR147 pKa = 11.84HH148 pKa = 5.4TIFGDD153 pKa = 3.44DD154 pKa = 3.3MGKK157 pKa = 9.81VDD159 pKa = 4.15EE160 pKa = 4.57AAVRR164 pKa = 11.84ATASDD169 pKa = 2.96ICGEE173 pKa = 4.01FKK175 pKa = 10.74INEE178 pKa = 4.14HH179 pKa = 5.86HH180 pKa = 6.76TNALVYY186 pKa = 9.94AAAYY190 pKa = 10.28LAMTPDD196 pKa = 3.28QRR198 pKa = 11.84SIDD201 pKa = 4.17SVKK204 pKa = 10.37LAYY207 pKa = 9.99NPKK210 pKa = 8.46SQARR214 pKa = 11.84RR215 pKa = 11.84TLVSAIRR222 pKa = 11.84EE223 pKa = 4.18NKK225 pKa = 9.26AVAGFKK231 pKa = 10.61SLEE234 pKa = 4.18DD235 pKa = 3.79FF236 pKa = 4.71

Molecular weight: 27.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P22955|CAPSD_RCNMV Capsid protein OS=Red clover necrotic mosaic virus OX=12267 GN=ORF2 PE=1 SV=1
MM1 pKa = 7.4AVHH4 pKa = 6.32VEE6 pKa = 4.09NLSDD10 pKa = 4.26LAKK13 pKa = 10.18TNDD16 pKa = 3.45GVAVSLNRR24 pKa = 11.84YY25 pKa = 6.56TDD27 pKa = 3.42WKK29 pKa = 9.81CRR31 pKa = 11.84SGVSEE36 pKa = 4.77APLIPASMMSKK47 pKa = 8.26ITDD50 pKa = 3.71YY51 pKa = 11.79AKK53 pKa = 8.35TTAKK57 pKa = 10.38GNSVALNYY65 pKa = 8.76THH67 pKa = 6.73VVLSLAPTIGVAIPGHH83 pKa = 4.75VTVEE87 pKa = 4.4LINPNVEE94 pKa = 3.93GPFQVMSGQTLSWSPGAGKK113 pKa = 8.96PCLMIFSVHH122 pKa = 5.83HH123 pKa = 5.56QLNSDD128 pKa = 3.73HH129 pKa = 6.98EE130 pKa = 4.32PFRR133 pKa = 11.84VRR135 pKa = 11.84ITNTGIPTKK144 pKa = 10.28KK145 pKa = 10.28SYY147 pKa = 10.75ARR149 pKa = 11.84CHH151 pKa = 6.39AYY153 pKa = 9.08WGFDD157 pKa = 2.58VGTRR161 pKa = 11.84HH162 pKa = 6.72RR163 pKa = 11.84YY164 pKa = 8.8YY165 pKa = 10.47KK166 pKa = 10.32SEE168 pKa = 3.93PARR171 pKa = 11.84LIEE174 pKa = 5.18LEE176 pKa = 3.93VGYY179 pKa = 10.52QRR181 pKa = 11.84TLLSSIKK188 pKa = 10.41AVEE191 pKa = 4.51AYY193 pKa = 10.42VQFTFDD199 pKa = 3.41TSRR202 pKa = 11.84MEE204 pKa = 5.38KK205 pKa = 10.36NPQLCTKK212 pKa = 10.03SNVNIIPPKK221 pKa = 10.59AEE223 pKa = 3.82TGSIRR228 pKa = 11.84GIAPPLSVVPNQGRR242 pKa = 11.84EE243 pKa = 4.25SKK245 pKa = 10.62VLKK248 pKa = 10.48QKK250 pKa = 10.92GGTGSKK256 pKa = 5.98TTKK259 pKa = 10.37LPSLEE264 pKa = 4.29PSSGSSSGLSMSRR277 pKa = 11.84RR278 pKa = 11.84SHH280 pKa = 6.69RR281 pKa = 11.84NVLNSSIPIKK291 pKa = 10.58RR292 pKa = 11.84NQDD295 pKa = 3.45GNWLGDD301 pKa = 3.59HH302 pKa = 6.93LSDD305 pKa = 4.58KK306 pKa = 11.22GRR308 pKa = 11.84VTDD311 pKa = 4.64PNPEE315 pKa = 4.01RR316 pKa = 11.84LL317 pKa = 3.58

Molecular weight: 34.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

1

4

1659

236

767

414.8

46.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.113 ± 0.673

2.049 ± 0.324

5.425 ± 0.68

5.184 ± 0.885

4.34 ± 0.669

6.088 ± 0.606

2.11 ± 0.354

5.184 ± 0.135

6.751 ± 0.612

7.957 ± 0.503

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.773 ± 0.352

4.702 ± 0.26

4.762 ± 0.772

3.737 ± 0.443

6.269 ± 0.46

8.258 ± 1.349

5.546 ± 1.203

7.715 ± 0.475

1.386 ± 0.221

2.652 ± 0.13
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski