Amino acid dipepetide frequency for Clavibacter phage CN1A

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.712AlaAla: 13.712 ± 1.451
0.18AlaCys: 0.18 ± 0.107
5.389AlaAsp: 5.389 ± 0.575
4.91AlaGlu: 4.91 ± 0.612
3.772AlaPhe: 3.772 ± 0.485
8.383AlaGly: 8.383 ± 1.186
1.736AlaHis: 1.736 ± 0.384
4.85AlaIle: 4.85 ± 0.514
5.09AlaLys: 5.09 ± 0.656
8.862AlaLeu: 8.862 ± 0.738
2.874AlaMet: 2.874 ± 0.429
4.371AlaAsn: 4.371 ± 0.566
5.03AlaPro: 5.03 ± 0.524
3.652AlaGln: 3.652 ± 0.614
7.245AlaArg: 7.245 ± 0.942
5.988AlaSer: 5.988 ± 0.743
7.185AlaThr: 7.185 ± 0.867
7.425AlaVal: 7.425 ± 0.621
3.054AlaTrp: 3.054 ± 0.489
3.054AlaTyr: 3.054 ± 0.357
0.0AlaXaa: 0.0 ± 0.0
Cys
0.299CysAla: 0.299 ± 0.189
0.0CysCys: 0.0 ± 0.0
0.18CysAsp: 0.18 ± 0.093
0.539CysGlu: 0.539 ± 0.189
0.18CysPhe: 0.18 ± 0.099
0.12CysGly: 0.12 ± 0.09
0.12CysHis: 0.12 ± 0.108
0.18CysIle: 0.18 ± 0.111
0.18CysLys: 0.18 ± 0.117
0.659CysLeu: 0.659 ± 0.262
0.0CysMet: 0.0 ± 0.0
0.06CysAsn: 0.06 ± 0.057
0.299CysPro: 0.299 ± 0.152
0.12CysGln: 0.12 ± 0.095
0.539CysArg: 0.539 ± 0.25
0.299CysSer: 0.299 ± 0.151
0.419CysThr: 0.419 ± 0.171
0.299CysVal: 0.299 ± 0.152
0.18CysTrp: 0.18 ± 0.142
0.12CysTyr: 0.12 ± 0.077
0.0CysXaa: 0.0 ± 0.0
Asp
6.167AspAla: 6.167 ± 0.68
0.299AspCys: 0.299 ± 0.131
3.173AspAsp: 3.173 ± 0.527
4.67AspGlu: 4.67 ± 0.555
2.694AspPhe: 2.694 ± 0.459
6.886AspGly: 6.886 ± 0.657
0.958AspHis: 0.958 ± 0.341
3.533AspIle: 3.533 ± 0.502
2.754AspLys: 2.754 ± 0.414
5.688AspLeu: 5.688 ± 0.605
1.796AspMet: 1.796 ± 0.294
1.916AspAsn: 1.916 ± 0.349
2.874AspPro: 2.874 ± 0.45
1.796AspGln: 1.796 ± 0.325
3.832AspArg: 3.832 ± 0.443
4.012AspSer: 4.012 ± 0.46
3.952AspThr: 3.952 ± 0.386
3.473AspVal: 3.473 ± 0.374
1.198AspTrp: 1.198 ± 0.333
2.096AspTyr: 2.096 ± 0.414
0.0AspXaa: 0.0 ± 0.0
Glu
6.946GluAla: 6.946 ± 0.802
0.359GluCys: 0.359 ± 0.189
3.712GluAsp: 3.712 ± 0.521
3.293GluGlu: 3.293 ± 0.523
2.275GluPhe: 2.275 ± 0.429
4.551GluGly: 4.551 ± 0.648
0.958GluHis: 0.958 ± 0.24
3.533GluIle: 3.533 ± 0.439
2.275GluLys: 2.275 ± 0.393
4.85GluLeu: 4.85 ± 0.521
1.497GluMet: 1.497 ± 0.265
2.515GluAsn: 2.515 ± 0.409
2.694GluPro: 2.694 ± 0.428
1.736GluGln: 1.736 ± 0.365
3.892GluArg: 3.892 ± 0.465
2.934GluSer: 2.934 ± 0.446
3.114GluThr: 3.114 ± 0.362
4.97GluVal: 4.97 ± 0.545
1.317GluTrp: 1.317 ± 0.297
1.916GluTyr: 1.916 ± 0.372
0.0GluXaa: 0.0 ± 0.0
Phe
3.593PheAla: 3.593 ± 0.576
0.12PheCys: 0.12 ± 0.094
2.694PheAsp: 2.694 ± 0.369
2.635PheGlu: 2.635 ± 0.41
0.898PhePhe: 0.898 ± 0.212
3.233PheGly: 3.233 ± 0.406
0.539PheHis: 0.539 ± 0.16
1.736PheIle: 1.736 ± 0.397
1.916PheLys: 1.916 ± 0.467
1.916PheLeu: 1.916 ± 0.41
0.419PheMet: 0.419 ± 0.174
1.257PheAsn: 1.257 ± 0.264
1.557PhePro: 1.557 ± 0.439
0.958PheGln: 0.958 ± 0.282
1.736PheArg: 1.736 ± 0.263
2.096PheSer: 2.096 ± 0.313
2.934PheThr: 2.934 ± 0.396
2.096PheVal: 2.096 ± 0.325
0.599PheTrp: 0.599 ± 0.186
1.198PheTyr: 1.198 ± 0.199
0.0PheXaa: 0.0 ± 0.0
Gly
8.083GlyAla: 8.083 ± 0.987
0.359GlyCys: 0.359 ± 0.157
5.808GlyAsp: 5.808 ± 0.655
4.251GlyGlu: 4.251 ± 0.611
2.994GlyPhe: 2.994 ± 0.343
6.407GlyGly: 6.407 ± 0.785
1.317GlyHis: 1.317 ± 0.35
4.072GlyIle: 4.072 ± 0.395
4.79GlyLys: 4.79 ± 0.478
6.946GlyLeu: 6.946 ± 0.885
2.096GlyMet: 2.096 ± 0.407
3.892GlyAsn: 3.892 ± 0.418
2.275GlyPro: 2.275 ± 0.32
2.455GlyGln: 2.455 ± 0.434
4.551GlyArg: 4.551 ± 0.479
5.329GlySer: 5.329 ± 0.648
5.928GlyThr: 5.928 ± 0.784
7.604GlyVal: 7.604 ± 0.842
1.796GlyTrp: 1.796 ± 0.282
3.533GlyTyr: 3.533 ± 0.703
0.0GlyXaa: 0.0 ± 0.0
His
1.617HisAla: 1.617 ± 0.351
0.12HisCys: 0.12 ± 0.139
1.377HisAsp: 1.377 ± 0.317
1.138HisGlu: 1.138 ± 0.318
0.419HisPhe: 0.419 ± 0.181
1.317HisGly: 1.317 ± 0.272
0.24HisHis: 0.24 ± 0.134
1.317HisIle: 1.317 ± 0.299
0.958HisLys: 0.958 ± 0.323
1.377HisLeu: 1.377 ± 0.379
0.359HisMet: 0.359 ± 0.146
0.299HisAsn: 0.299 ± 0.128
1.138HisPro: 1.138 ± 0.272
0.659HisGln: 0.659 ± 0.189
1.198HisArg: 1.198 ± 0.301
0.479HisSer: 0.479 ± 0.165
1.078HisThr: 1.078 ± 0.26
0.958HisVal: 0.958 ± 0.261
0.24HisTrp: 0.24 ± 0.12
0.299HisTyr: 0.299 ± 0.131
0.0HisXaa: 0.0 ± 0.0
Ile
4.97IleAla: 4.97 ± 0.536
0.18IleCys: 0.18 ± 0.111
3.892IleAsp: 3.892 ± 0.498
3.832IleGlu: 3.832 ± 0.608
0.898IlePhe: 0.898 ± 0.249
3.533IleGly: 3.533 ± 0.438
1.018IleHis: 1.018 ± 0.212
1.736IleIle: 1.736 ± 0.339
2.754IleLys: 2.754 ± 0.464
2.814IleLeu: 2.814 ± 0.345
0.778IleMet: 0.778 ± 0.218
2.096IleAsn: 2.096 ± 0.439
2.994IlePro: 2.994 ± 0.373
2.455IleGln: 2.455 ± 0.329
3.114IleArg: 3.114 ± 0.358
2.814IleSer: 2.814 ± 0.477
4.191IleThr: 4.191 ± 0.591
4.251IleVal: 4.251 ± 0.479
0.778IleTrp: 0.778 ± 0.224
0.838IleTyr: 0.838 ± 0.186
0.0IleXaa: 0.0 ± 0.0
Lys
5.389LysAla: 5.389 ± 0.689
0.18LysCys: 0.18 ± 0.083
2.455LysAsp: 2.455 ± 0.414
2.635LysGlu: 2.635 ± 0.461
2.036LysPhe: 2.036 ± 0.559
3.533LysGly: 3.533 ± 0.503
0.838LysHis: 0.838 ± 0.255
2.455LysIle: 2.455 ± 0.402
1.856LysLys: 1.856 ± 0.333
4.191LysLeu: 4.191 ± 0.493
0.958LysMet: 0.958 ± 0.307
1.736LysAsn: 1.736 ± 0.362
2.215LysPro: 2.215 ± 0.318
1.976LysGln: 1.976 ± 0.338
2.754LysArg: 2.754 ± 0.376
2.754LysSer: 2.754 ± 0.378
3.712LysThr: 3.712 ± 0.465
3.293LysVal: 3.293 ± 0.451
1.018LysTrp: 1.018 ± 0.282
0.958LysTyr: 0.958 ± 0.255
0.0LysXaa: 0.0 ± 0.0
Leu
8.143LeuAla: 8.143 ± 0.944
0.719LeuCys: 0.719 ± 0.347
5.628LeuAsp: 5.628 ± 0.623
4.79LeuGlu: 4.79 ± 0.732
2.934LeuPhe: 2.934 ± 0.326
6.646LeuGly: 6.646 ± 0.849
1.018LeuHis: 1.018 ± 0.294
4.012LeuIle: 4.012 ± 0.476
3.712LeuLys: 3.712 ± 0.664
5.988LeuLeu: 5.988 ± 0.792
1.198LeuMet: 1.198 ± 0.28
3.413LeuAsn: 3.413 ± 0.475
3.413LeuPro: 3.413 ± 0.467
2.575LeuGln: 2.575 ± 0.409
4.97LeuArg: 4.97 ± 0.56
5.389LeuSer: 5.389 ± 0.591
4.97LeuThr: 4.97 ± 0.521
5.808LeuVal: 5.808 ± 0.572
1.198LeuTrp: 1.198 ± 0.283
2.455LeuTyr: 2.455 ± 0.417
0.0LeuXaa: 0.0 ± 0.0
Met
2.335MetAla: 2.335 ± 0.385
0.18MetCys: 0.18 ± 0.088
1.317MetAsp: 1.317 ± 0.251
1.078MetGlu: 1.078 ± 0.319
0.719MetPhe: 0.719 ± 0.251
2.156MetGly: 2.156 ± 0.355
0.419MetHis: 0.419 ± 0.164
1.317MetIle: 1.317 ± 0.276
1.078MetLys: 1.078 ± 0.232
1.736MetLeu: 1.736 ± 0.273
0.719MetMet: 0.719 ± 0.229
1.377MetAsn: 1.377 ± 0.308
0.359MetPro: 0.359 ± 0.137
0.898MetGln: 0.898 ± 0.198
1.497MetArg: 1.497 ± 0.379
1.796MetSer: 1.796 ± 0.362
2.335MetThr: 2.335 ± 0.478
0.958MetVal: 0.958 ± 0.256
0.12MetTrp: 0.12 ± 0.08
0.479MetTyr: 0.479 ± 0.188
0.0MetXaa: 0.0 ± 0.0
Asn
3.772AsnAla: 3.772 ± 0.407
0.06AsnCys: 0.06 ± 0.064
2.335AsnAsp: 2.335 ± 0.369
2.694AsnGlu: 2.694 ± 0.404
1.317AsnPhe: 1.317 ± 0.291
3.533AsnGly: 3.533 ± 0.345
0.539AsnHis: 0.539 ± 0.196
2.635AsnIle: 2.635 ± 0.423
1.617AsnLys: 1.617 ± 0.312
2.934AsnLeu: 2.934 ± 0.37
0.958AsnMet: 0.958 ± 0.226
1.856AsnAsn: 1.856 ± 0.432
2.036AsnPro: 2.036 ± 0.254
1.617AsnGln: 1.617 ± 0.301
2.635AsnArg: 2.635 ± 0.37
2.215AsnSer: 2.215 ± 0.377
3.293AsnThr: 3.293 ± 0.416
2.575AsnVal: 2.575 ± 0.455
0.359AsnTrp: 0.359 ± 0.143
1.138AsnTyr: 1.138 ± 0.286
0.0AsnXaa: 0.0 ± 0.0
Pro
4.79ProAla: 4.79 ± 0.579
0.24ProCys: 0.24 ± 0.128
2.934ProAsp: 2.934 ± 0.385
4.191ProGlu: 4.191 ± 0.438
1.557ProPhe: 1.557 ± 0.402
3.173ProGly: 3.173 ± 0.384
0.958ProHis: 0.958 ± 0.285
1.976ProIle: 1.976 ± 0.27
1.796ProLys: 1.796 ± 0.377
3.533ProLeu: 3.533 ± 0.614
1.138ProMet: 1.138 ± 0.264
1.916ProAsn: 1.916 ± 0.39
1.557ProPro: 1.557 ± 0.282
1.317ProGln: 1.317 ± 0.289
1.796ProArg: 1.796 ± 0.367
2.575ProSer: 2.575 ± 0.412
3.473ProThr: 3.473 ± 0.549
3.293ProVal: 3.293 ± 0.416
0.419ProTrp: 0.419 ± 0.135
1.018ProTyr: 1.018 ± 0.247
0.0ProXaa: 0.0 ± 0.0
Gln
2.934GlnAla: 2.934 ± 0.447
0.12GlnCys: 0.12 ± 0.084
1.856GlnAsp: 1.856 ± 0.317
2.275GlnGlu: 2.275 ± 0.363
1.138GlnPhe: 1.138 ± 0.235
3.114GlnGly: 3.114 ± 0.321
0.479GlnHis: 0.479 ± 0.223
2.395GlnIle: 2.395 ± 0.404
1.198GlnLys: 1.198 ± 0.286
3.772GlnLeu: 3.772 ± 0.801
0.898GlnMet: 0.898 ± 0.193
1.257GlnAsn: 1.257 ± 0.246
1.677GlnPro: 1.677 ± 0.337
1.317GlnGln: 1.317 ± 0.283
2.096GlnArg: 2.096 ± 0.411
1.736GlnSer: 1.736 ± 0.275
1.856GlnThr: 1.856 ± 0.343
3.173GlnVal: 3.173 ± 0.351
0.838GlnTrp: 0.838 ± 0.202
0.778GlnTyr: 0.778 ± 0.242
0.0GlnXaa: 0.0 ± 0.0
Arg
7.006ArgAla: 7.006 ± 0.678
0.299ArgCys: 0.299 ± 0.135
4.131ArgAsp: 4.131 ± 0.618
3.473ArgGlu: 3.473 ± 0.516
2.395ArgPhe: 2.395 ± 0.427
4.251ArgGly: 4.251 ± 0.528
1.018ArgHis: 1.018 ± 0.283
2.994ArgIle: 2.994 ± 0.394
3.353ArgLys: 3.353 ± 0.455
5.03ArgLeu: 5.03 ± 0.534
1.796ArgMet: 1.796 ± 0.406
1.856ArgAsn: 1.856 ± 0.298
2.934ArgPro: 2.934 ± 0.453
2.994ArgGln: 2.994 ± 0.385
3.892ArgArg: 3.892 ± 0.556
3.353ArgSer: 3.353 ± 0.347
3.712ArgThr: 3.712 ± 0.583
4.371ArgVal: 4.371 ± 0.507
0.958ArgTrp: 0.958 ± 0.291
1.796ArgTyr: 1.796 ± 0.258
0.0ArgXaa: 0.0 ± 0.0
Ser
6.287SerAla: 6.287 ± 0.804
0.24SerCys: 0.24 ± 0.129
4.251SerAsp: 4.251 ± 0.527
3.173SerGlu: 3.173 ± 0.413
2.036SerPhe: 2.036 ± 0.294
6.107SerGly: 6.107 ± 0.806
0.838SerHis: 0.838 ± 0.238
2.994SerIle: 2.994 ± 0.38
2.874SerLys: 2.874 ± 0.478
4.431SerLeu: 4.431 ± 0.516
1.617SerMet: 1.617 ± 0.331
2.455SerAsn: 2.455 ± 0.452
1.916SerPro: 1.916 ± 0.317
1.796SerGln: 1.796 ± 0.34
3.652SerArg: 3.652 ± 0.398
3.293SerSer: 3.293 ± 0.613
3.772SerThr: 3.772 ± 0.652
4.73SerVal: 4.73 ± 0.559
1.018SerTrp: 1.018 ± 0.234
1.257SerTyr: 1.257 ± 0.22
0.0SerXaa: 0.0 ± 0.0
Thr
7.964ThrAla: 7.964 ± 0.973
0.719ThrCys: 0.719 ± 0.249
3.772ThrAsp: 3.772 ± 0.396
3.353ThrGlu: 3.353 ± 0.405
2.215ThrPhe: 2.215 ± 0.414
6.946ThrGly: 6.946 ± 0.674
1.377ThrHis: 1.377 ± 0.241
4.131ThrIle: 4.131 ± 0.584
2.994ThrLys: 2.994 ± 0.435
4.67ThrLeu: 4.67 ± 0.576
1.317ThrMet: 1.317 ± 0.294
2.455ThrAsn: 2.455 ± 0.355
3.952ThrPro: 3.952 ± 0.697
2.275ThrGln: 2.275 ± 0.415
4.251ThrArg: 4.251 ± 0.407
3.892ThrSer: 3.892 ± 0.591
5.389ThrThr: 5.389 ± 0.79
5.748ThrVal: 5.748 ± 0.607
1.257ThrTrp: 1.257 ± 0.274
1.736ThrTyr: 1.736 ± 0.302
0.0ThrXaa: 0.0 ± 0.0
Val
7.784ValAla: 7.784 ± 0.591
0.18ValCys: 0.18 ± 0.11
5.688ValAsp: 5.688 ± 0.597
3.892ValGlu: 3.892 ± 0.415
2.096ValPhe: 2.096 ± 0.416
5.748ValGly: 5.748 ± 0.607
1.317ValHis: 1.317 ± 0.285
2.395ValIle: 2.395 ± 0.391
3.593ValLys: 3.593 ± 0.491
5.509ValLeu: 5.509 ± 0.595
1.377ValMet: 1.377 ± 0.27
3.772ValAsn: 3.772 ± 0.451
3.054ValPro: 3.054 ± 0.44
2.215ValGln: 2.215 ± 0.432
4.91ValArg: 4.91 ± 0.568
5.209ValSer: 5.209 ± 0.644
5.748ValThr: 5.748 ± 0.653
6.048ValVal: 6.048 ± 0.611
1.198ValTrp: 1.198 ± 0.284
3.054ValTyr: 3.054 ± 0.43
0.0ValXaa: 0.0 ± 0.0
Trp
1.916TrpAla: 1.916 ± 0.292
0.12TrpCys: 0.12 ± 0.078
1.377TrpAsp: 1.377 ± 0.255
1.138TrpGlu: 1.138 ± 0.256
0.599TrpPhe: 0.599 ± 0.207
1.736TrpGly: 1.736 ± 0.327
0.359TrpHis: 0.359 ± 0.12
0.838TrpIle: 0.838 ± 0.249
0.898TrpLys: 0.898 ± 0.213
1.138TrpLeu: 1.138 ± 0.227
0.539TrpMet: 0.539 ± 0.216
0.659TrpAsn: 0.659 ± 0.197
0.599TrpPro: 0.599 ± 0.203
0.539TrpGln: 0.539 ± 0.239
1.018TrpArg: 1.018 ± 0.244
1.138TrpSer: 1.138 ± 0.219
1.078TrpThr: 1.078 ± 0.266
1.617TrpVal: 1.617 ± 0.311
0.299TrpTrp: 0.299 ± 0.138
0.479TrpTyr: 0.479 ± 0.195
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.054TyrAla: 3.054 ± 0.362
0.12TyrCys: 0.12 ± 0.091
1.796TyrAsp: 1.796 ± 0.263
1.377TyrGlu: 1.377 ± 0.286
0.958TyrPhe: 0.958 ± 0.235
3.114TyrGly: 3.114 ± 0.623
0.539TyrHis: 0.539 ± 0.202
0.838TyrIle: 0.838 ± 0.175
1.317TyrLys: 1.317 ± 0.25
2.934TyrLeu: 2.934 ± 0.365
0.479TyrMet: 0.479 ± 0.129
0.958TyrAsn: 0.958 ± 0.257
1.078TyrPro: 1.078 ± 0.22
1.557TyrGln: 1.557 ± 0.252
2.036TyrArg: 2.036 ± 0.279
1.437TyrSer: 1.437 ± 0.278
2.275TyrThr: 2.275 ± 0.386
2.096TyrVal: 2.096 ± 0.398
0.24TyrTrp: 0.24 ± 0.102
0.898TyrTyr: 0.898 ± 0.201
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 78 proteins (16702 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski